A5114889684- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- MicroRNA in disease regulation
- Mycobacterium research and diagnosis
- Helicobacter pylori-related gastroenterology studies
- Barrier Structure and Function Studies
- Circular RNAs in diseases
- Gastric Cancer Management and Outcomes
- Cancer, Lipids, and Metabolism
- Wnt/β-catenin signaling in development and cancer
- Tissue Engineering and Regenerative Medicine
- Digestive system and related health
- Wound Healing and Treatments
- Cancer Research and Treatments
- Enhanced Recovery After Surgery
- Autophagy in Disease and Therapy
- RNA regulation and disease
- Ion Channels and Receptors
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Plant Molecular Biology Research
- IL-33, ST2, and ILC Pathways
- Growth Hormone and Insulin-like Growth Factors
University of Maryland, Baltimore
2015-2024
Baltimore VA Medical Center
2012-2024
University of Maryland, College Park
2023
Veterans Health Administration
2015-2022
University of Baltimore
2011-2013
The disruption of the intestinal epithelial barrier function occurs commonly in various pathologies, but exact mechanisms responsible are unclear. H19 long noncoding RNA (lncRNA) regulates expression different genes and has been implicated human genetic disorders cancer. Here, we report that plays an important role controlling by serving as a precursor for microRNA 675 (miR-675). overexpression increased cellular abundance miR-675, which turn destabilized repressed translation mRNAs encoding...
Epithelial cells line the intestinal mucosa and form an important barrier to a wide array of noxious substances in lumen. Disruption integrity occurs commonly various pathologies. Long noncoding RNAs (lncRNAs) control diverse biological processes, but little is known about role lncRNAs regulation gut permeability. Here we show that lncRNA SPRY4-IT1 regulates epithelial function by altering expression tight junction (TJ) proteins. silencing led dysfunction cultured decreasing stability mRNAs...
Stromal interaction molecule 1 (Stim1) functions as a sensor of Ca 2+ within stores and plays an essential role in the activation store-operated entry (SOCE). Although lowering Stim1 levels reduces inhibits intestinal epithelial repair after wounding, mechanisms that control expression remain unknown. Here, we show cellular abundance is controlled posttranscriptionally via factors associate with 3′-untranslated region (3′-UTR) stim1 mRNA. MicroRNA-195 (miR-195) RNA-binding protein HuR...
Occludin is a transmembrane tight junction (TJ) protein that plays an important role in TJ assembly and regulation of the epithelial barrier function, but mechanisms underlying its post-transcriptional are unknown. The RNA-binding HuR modulates stability translation many target mRNAs. Here, we investigated occludin expression therefore intestinal function. bound 3'-untranslated region mRNA enhanced translation. association with depended on Chk2-dependent phosphorylation. Reduced...
Background & AimsThe protective intestinal mucosal barrier consists of multiple elements including mucus and epithelial layers immune defense; nonetheless, dysfunction is common in various disorders. The imprinted developmentally regulated long noncoding RNA H19 involved many cell processes diseases. Here, we investigated the role regulating Paneth goblet cells autophagy, its impact on induced by septic stress.MethodsStudies were conducted H19-deficient (H19-/-) mice, tissues from patients...
RNA-binding proteins CUG-binding protein 1 (CUGBP1) and HuR are highly expressed in epithelial tissues modulate the stability translation of target mRNAs. Here we present evidence that CUGBP1 jointly regulate occludin play a crucial role maintenance tight junction (TJ) integrity intestinal cell monolayer. competed for association with same 3'-untranslated region element regulated competitively opposite directions. overexpression decreased binding to mRNA, repressed translation, compromised...
The mammalian intestinal epithelium establishes a selectively permeable barrier that supports nutrient absorption and prevents intrusion by noxious luminal substances microbiota. effectiveness integrity of the function are tightly regulated via well-controlled mechanisms. Long noncoding RNAs transcribed from ultraconserved regions (T-UCRs) control diverse cellular processes, but their roles in regulation gut permeability remain largely unknown. Here we report T-UCR uc.173 enhances epithelial...
Intestinal Tuft cells sense luminal contents to influence the mucosal immune response against eukaryotic infection. Paneth secrete antimicrobial proteins as part of protective barrier. Defects in and occur commonly various gut disorders. MicroRNA-195 (miR-195) regulates stability translation target mRNAs is involved many aspects cell processes pathologies. Here, we reported posttranscriptional mechanisms by which miR-195 function small intestinal epithelium. Mucosal tissues from epithelial...
Insulin-like growth factor type 2 (IGF2) receptor (IGF2R) recognizes mannose 6-phosphate-containing molecules and IGF2 plays an important role in many pathophysiological processes, including gut mucosal adaptation. However, the mechanisms that control cellular IGF2R abundance are poorly known. MicroRNAs (miRNAs) RNA-binding proteins (RBPs) critically regulate gene expression programs mammalian cells by modulating stability translation of target mRNAs. Here we report miRNA 195 (miR-195) RBP...
Defects in intestinal epithelial integrity occur commonly various pathologies. miR-222 is implicated many aspects of cellular function and plays an important role several diseases, but its exact biological the epithelium underexplored. We generated mice with tissue-specific overexpression to investigate physiology diseases vivo. Transgenic expression inhibited mucosal growth increased susceptibility apoptosis small intestine, thus leading atrophy. The miR-222-elevated was vulnerable...
Intestinal epithelial integrity is commonly disrupted in patients with critical disorders, but the exact underlying mechanisms are unclear. Long noncoding RNAs transcribed from ultraconserved regions (T-UCRs) control different cell functions and involved pathologies. Here, we investigated role of T-UCRs intestinal homeostasis identified T-UCR uc.230 as a major regulator renewal, apoptosis, barrier function. Compared controls, mucosal tissues ulcerative colitis mice or fasted for 48 hours had...
The human intestinal epithelium has an impressive ability to respond insults and its homeostasis is maintained by well-regulated mechanisms under various pathophysiological conditions. Nonetheless, acute injury inhibited regeneration of the occur commonly in critically ill surgical patients, leading translocation luminal toxic substances bacteria bloodstream. Effective therapies for preservation epithelial integrity prevention mucosal hemorrhage gut barrier dysfunction are limited, primarily...
Rapid self-renewal of the intestinal epithelium requires activity stem cells (ISCs) that are intermingled with Paneth (PCs) at crypt base. PCs provide multiple secreted and surface-bound niche signals play an important role in regulation ISC proliferation. Here, we show control PC function by RNA-binding protein HuR via mitochondria affects mucosal growth altering activity. Targeted deletion mice disrupted gene expression profiles, reduced PC-derived factors, impaired function, leading to...
Early gut epithelial restitution reseals superficial wounds after acute injury, but the exact mechanism underlying this rapid mucosal repair remains largely unknown. MicroRNA-195 (miR-195) is highly expressed in epithelium and involved many aspects of pathobiology. Actin-related proteins (ARPs) are key components essential for stimulation actin polymerization regulate cell motility. Here, we reported that miR-195 modulates early intestinal by altering ARP-2 expression at translation level....
Paneth cells at the bottom of small intestinal crypts secrete antimicrobial peptides, enzymes, and growth factors contribute to pathogen clearance maintenance stem cell niche. Loss their dysfunction occur commonly in various pathologies, but mechanism underlying control function remains largely unknown. Here, we identified microRNA-195 (miR-195) as a repressor development activity by altering SOX9 translation via interaction with RNA-binding protein HuR. Tissue-specific transgenic expression...