A5013798827- Insect and Arachnid Ecology and Behavior
- Cancer-related Molecular Pathways
- Neurobiology and Insect Physiology Research
- Lepidoptera: Biology and Taxonomy
- Advanced Breast Cancer Therapies
- Plant and animal studies
- Ubiquitin and proteasome pathways
- Synthesis and properties of polymers
- Semiconductor materials and devices
- Membrane Separation and Gas Transport
- Biomimetic flight and propulsion mechanisms
- Magnetic properties of thin films
- Nuclear reactor physics and engineering
- Entomological Studies and Ecology
- Magnetic Properties and Applications
- Semiconductor Quantum Structures and Devices
- Diamond and Carbon-based Materials Research
- Plant Reproductive Biology
- Engineering Applied Research
- Nuclear Materials and Properties
- Aerospace Engineering and Energy Systems
- Advanced Combustion Engine Technologies
- Melanoma and MAPK Pathways
- Genetics, Aging, and Longevity in Model Organisms
- Semiconductor Lasers and Optical Devices
Case Western Reserve University
2020-2025
University School
2025
Kindai University
2021-2024
Toyama University Hospital
2024
Nara Institute of Science and Technology
2008-2023
Osaka University
2011-2022
University Hospitals of Cleveland
2022
National Institutes of Natural Sciences
2021
National Institute for Basic Biology
2017-2021
Tokyo University of Pharmacy and Life Sciences
2020
Dysregulation of the p16-cyclin D1-CDK4/6-Rb pathway occurs frequently in melanoma; however, therapeutic efficacy CDK4/6 inhibition remains to be critically evaluated. We demonstrate that inhibits melanoma progression through induction senescence. Palbociclib, a specific inhibitor, rapidly induces cell cycle arrest within 24 hours and continued exposure for 8 days or longer The senescence correlates with mTOR more specifically mTORC1 signaling. Vemurafenib, BRAF(V600E) has significant...
Purpose: Loss of cell-cycle control is a hallmark cancer, which can be targeted with agents, including cyclin-dependent kinase-4/6 (CDK4/6) kinase inhibitors that impinge upon the G1-S checkpoint via maintaining activity retinoblastoma tumor suppressor (RB). This class drugs under clinical investigation for various solid types and has recently been FDA-approved treatment breast cancer. However, development therapeutic resistance not uncommon.Experimental Design: In this study, palbociclib (a...
Abstract The dysregulation of Fbxo4-cyclin D1 axis occurs at high frequency in esophageal squamous cell carcinoma (ESCC), where it promotes ESCC development and progression. However, defining a therapeutic vulnerability that results from this has remained elusive. Here we demonstrate Rb mTORC1 contribute to Gln-addiction upon the axis, which leads reprogramming cellular metabolism. This is characterized by reduced energy production increased sensitivity cells combined treatment with CB-839...
A transparent wing of a hawkmoth, Cephonodes hylas, has highly ordered array nanosized protuberances in its surface. This protuberance almost the same morphology as that “corneal nipple array”, which is suggested to function an antireflective device light, using scaled model experiment, theoretical calculation, and comparative way. For direct study this array, we prepared artificial without compared it with intact one protuberances. Directly measuring light reflectance spectra one,...
The unfolded protein response (UPR) regulates cell fate following exposure of cells to endoplasmic reticulum stresses. PERK, a UPR kinase, synthesis and while linked with survival, exhibits activities associated both tumor progression suppression. For example, lacking PERK are sensitive UPR-dependent death, acute activation triggers apoptosis cycle arrest, which would be expected contribute suppressive activity. We have evaluated these in the BRAF-dependent melanoma provide evidence...
The transport properties of ionic liquid (IL)/sulfonated polyimide (SPI) composite membranes for CO2 separation were explored in relation to their nanostructures. 1-Butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)amide ([C4mim][NTf2]), 1-butyl-3-methylimidazolium hexafluorophosphate ([C4mim]PF6), and 1-butyl-1-methylpyrrolidinium ([P14][NTf2]) selected as the ILs. These enable favorable superior mechanical properties. SPI is plasticized by IL, which induces a decrease elastic modulus;...
We identified unique molecular mechanisms of resistance to CDK4/6 inhibitors, an area intense biomedical investigation.
Abstract The cyclin D1-Cyclin-Dependent Kinases 4 and 6 (CDK4/6) complex is crucial for the development of melanoma. We previously demonstrated that targeting CDK4/6 using small molecule inhibitors (CDK4/6i) suppresses BrafV600E melanoma growth in vitro vivo through induction cellular senescence. However, clinical trials investigating CDK4/6i have not yielded successful outcomes, underscoring necessity to enhance therapeutic efficacy CDK4/6i. Accumulated research has shown while senescence...
The fifth component (CSN5) of the mammalian COP9 signalosome complex plays an essential role in cell proliferation and senescence, but its molecular mediator remains to be determined. Here, we searched for interactors among various cycle regulators found that CSN5, not CSN holo-complex, bound CDK2 vivo vitro. Depletion CSN5 enhanced phosphorylation by Akt, resulting cytoplasmic accumulation together with cyclin E a leptomycin B-resistant manner impaired retinoblastoma protein. Additional...
The risk of hepatitis B virus (HBV) reactivation with immune checkpoint inhibitors (ICIs) is an important issue that has not yet been fully investigated. ICI also expected to have antiviral effect on HBV due its tolerance inhibitory effect. We herein investigated the and administration.This study included 892 patients ICIs between September 2014 May 2021 at our hospital. frequency effects were investigated.Among who underwent ICI, 27 surface antigen (HBsAg) positive. was evaluated in 24...
Abstract A highly ordered nanocomposite was discovered in the transparent wing of a hawkmoth, Cephonodes hylas. The protuberances, nipple-like shaped and 250 nm height, are regular-hexagonally arranged with center-to-center distance 200 nm. This morphology is almost same as those “corneal nipple arrays” some insect eyes. Nanocomposites closely similar to that were also found wings other moths (Sphingidae Sesiidae) cicadae.
MINT‐8046253: Csn1 (uniprotkb:Q99LD4) physically interacts (MI:0914) with Csn5 (uniprotkb:O35864), Csn8 (uniprotkb:Q8VBV7), Csn3 (uniprotkb:O88543), Csn7b (uniprotkb:Q8BV13) and Csn6 (uniprotkb:O88545) by anti bait coimmunoprecipitation (MI:0006)
Abstract Background The ubiquitin ligase COP1, COnstitutively Photomorphogenic 1, functions in many biological responses mammalian cells, but its downstream pathway remains unclear. Results Here, we identified FIP200, a key regulator of autophagy, as novel COP1-interacting protein by yeast two-hybrid screening. interaction was confirmed GST-pulldown assay. Split-GFP analysis revealed that between COP1 and FIP200 predominantly occurred the cytoplasm enhanced cells treated with UV irradiation....
Abstract Background Immune checkpoint inhibitors have been reported to excellent therapeutic effects on various malignant tumors. However, immune‐related adverse events can occur, targeting organs. Case presentation A 49‐year‐old male with lung carcinoma was started carboplatin + pemetrexed nivolumab (every 3 weeks) ipilimumab 6 weeks), and nivolumab/ipilimumab administered in the 3rd course. Subsequently, fever fatigue developed, grade liver damage also noted, so he admitted Kindai...
Overexpression of JAB1 is observed in a variety human cancers, but how involved tumor development remained to be investigated. Here we analyzed mice with modified Jab1 expression. Mice ectopically expressing more stable form protein under the control constitutive promoter were rescued from embryonic lethality caused by Jab1(-/-) allele and developed myeloproliferative disorder gene dosage-dependent manner. Hematopoietic cells bone marrow transgenic had significantly larger stem cell...
Abstract Overexpression of D-type cyclins in human cancer frequently occurs as a result protein stabilization, emphasizing the importance identification machinery that regulates their ubiqutin-dependent degradation. Cyclin D3 is overexpressed ~50% Burkitt’s lymphoma correlating with mutation Thr-283. However, E3 ligase phosphorylated cyclin and whether stabilized, phosphorylation deficient mutant D3, has oncogenic activity are undefined. We describe SCF-Fbxl8 for Thr-283 D3....
Proteins often exist as ensembles of interconverting states in solution which are difficult to quantify. In the present manuscript we show that combination MS under nondenaturing conditions and AUC-SV (analytical ultracentrifugation sedimentation velocity) unambiguously clarifies a distribution hydrodynamic shapes assembled oligomers for NAP-1 (nucleosome assembly protein 1). established number associated units, was utilized input numerical analysis profiles. The revealed less than 1%...
Abstract Clinical evidence suggests that cyclin D1b, a variant of D1, is associated with tumor progression and poor outcome. However, the underlying molecular basis was unknown. Here, novel models were created to generate genetic switch from D1 D1b. Extensive analyses uncovered overlapping but non‐redundant functions D1b compared on developmental phenotypes, illustrated importance transcriptional regulatory in vivo . Data obtained identify as an oncogene, wherein expression under endogenous...