- Soybean genetics and cultivation
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Transgenic Plants and Applications
- Phytoestrogen effects and research
- Plant Reproductive Biology
- GABA and Rice Research
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Insect Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
- Genetically Modified Organisms Research
- Plant pathogens and resistance mechanisms
- Food composition and properties
- Lipid metabolism and biosynthesis
- Biofuel production and bioconversion
- Gene expression and cancer classification
- Cassava research and cyanide
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
Henan Academy of Agricultural Sciences
2010-2025
Ministry of Agriculture and Rural Affairs
2022
Zhoukou City Academy of Agricultural Sciences
2016
Shijiazhuang Academy of Agriculture and Forestry Sciences
2016
Luohe Academy of Agricultural Sciences
2016
Anyang Academy of Agricultural Sciences
2016
China Agricultural University
2007-2009
McDermott International (United States)
2005
The University of Texas Southwestern Medical Center
2002
Abstract Background Soybean is one of the most important oil crops in world. The domestication wild soybean has resulted significant changes seed content and size cultivated soybeans. To better understand molecular mechanisms formation accumulation, WDD01514 (E1), ZYD00463 (E2), two extreme progenies (E23 E171) derived from RILs were used for weighted gene coexpression network analysis (WGCNA) combined with transcriptome analysis. Results In this study, both weight E1 E171 significantly...
Soybean cyst nematode (SCN) disease caused by Heterodera glycines is one of the most devastating diseases in soybean ( Glycine max L.) and results significant yield losses annually worldwide. Breeding crops for resistance an eco-friendly approach to minimize these losses. In this study, SCN-resistant germplasm with excellent agronomic traits was obtained through cross-breeding between resistant sources local cultivars that were high-yielding but susceptible SCN China’s two major...
Abstract Soybean cyst nematode (SCN, Heterodera glycines ) is a major pest of soybean that spreading across production regions worldwide. Increased SCN virulence has recently been observed in both the United States and China. However, no study reported genome assembly for H. at chromosome scale. Herein, first chromosome‐level reference X12, an unusual race with high infection ability, presented. Using whole‐genome shotgun (WGS) sequencing, Pacific Biosciences (PacBio) Illumina paired‐end 10X...
bZIP transcription factors have been reported to play important roles in plant responses abiotic stresses. Here, we the cloning and characterization of a putative factor (ZmbZIP17) from maize inbred line Han21, which is up-regulated by drought treatment. The open reading frame sequence ZmbZIP17 was obtained using 5’RACE RT-PCR. Sequence analysis showed that encodes polypeptide 563 amino acids with predicted molecular mass 59.8 kDa pI 5.6. Southern blot exists as single copy gene genome....
Abstract The soybean cyst nematode (SCN), Heterodera glycines , is a serious economic threat to soybean-producing regions worldwide. A new SCN population (called race X12) was detected in Shanxi province, China. Race X12 could reproduce on all the indicator lines of both and (HG) type tests. average number females Lee68 (susceptible control) 171.40 with lowest Female Index (FI) 61.31 PI88788 highest FI 117.32 Pickett test. 323.17 44.18 97.83 PI548316 HG ZDD2315 ZDD24656 are elite resistant...
The continuous evolution and spread of virulent forms the soybean cyst nematode (SCN) driven by environment anthropogenic intervention is a serious threat to production worldwide, including China. Especially in China, implemented measures control SCN are insufficient for sustainable agricultural development yet. We summarized our knowledge about spatial distribution China virulence diversity main growing areas. To reveal genetic relatedness populations, we re-sequenced 53 genomes from...
Abstract Secreted and cell surface proteins play important roles in cancer are potential drug targets tumor markers. Here, we describe a large-scale analysis of the genes encoding secreted breast cancer. To identify these genes, developed novel signal sequence trap method called Escherichia coli ampicillin secretion (CAST). For CAST, constructed plasmid which β-lactamase was deleted such that it does not confer resistance. Eukaryotic cDNA libraries cloned into pCAST produced tens thousands...
Soybean cyst nematode (SCN, Heterodera glycines , HG) is one of the severe pests in plant-parasitic nematodes, which impairs root development and causes losses soybean production worldwide. Breeding SCN-resistant cultivars an important measure for securing harvests without affecting environment, as can be done with pesticides. The majority genetic resources plant pest resistances are found wild or closely related species often difficult to use breeding due crossing barriers close linkage...
The Maize Wip1 gene encodes a wound-induced Bowman-Birk inhibitor (BBI) protein which is type of serine protease inhibitor, and its expression induced by wounding or infection, conferring resistance against pathogens pests. In this study, the maize promoter was isolated function analyzed. Different truncated promoters were fused upstream GUS reporter transformed into Arabidopsis, tobacco rice plants. We found that (1) several led to strong activities in both transgenic Arabidopsis leaves,...
摘要:
Soybean cyst nematode (SCN; Heterodera glycines Ichinohe) is a highly destructive pathogen for soybean production worldwide. The use of resistant varieties the most effective way preventing yield loss. Handou 10 commercial variety with desirable agronomic traits and SCN resistance, however genes underlying resistance in are unknown. An F2:8 recombinant inbred line (RIL) population derived from cross between Zheng 9525 (susceptible) was developed its to HG type 2.5.7 (race 1) 1.2.5.7 2)...
Abstract Summary: ARROGANT (ARRay OrGANizing Tool) is a software tool developed to facilitate the identification, annotation and comparison of large collections genes or clones. The objective enable users compile gene/clone from different databases, allowing them design experiments analyze as well associated experimental data efficiently. can relate sequence identifiers their common reference using UniGene database, for two microarray experiments. has been successfully used expression colon...
Cooling is a technique employed to reduce the high glucose release from white rice by altering proportion of rapidly digestible (RDS), slowly (SDS), and resistant (RS) starches. Retrogradation gelatinised starches crystalline structure increases RS content that does not spike blood on consumption. The meta-analysis showed following different retrogradation durations, there were significant mean differences (MD), viz. an increase in (MD: 4.17 g/100 g, p < 0.00001) decrease RDS -7.09...
Soybean cyst nematode (SCN) is a serious damaging disease in soybean worldwide. Peking- and PI 88788-type sources of resistance are two most important germplasm used breeding resistant cultivars against this disease. However, until now, no comparisons constitutive resistances to between these types had been conducted, probably due the influences different backgrounds. In study, we pooled-sample analysis strategy minimize influence backgrounds directly compared molecular mechanisms underlying...