A5039585773- Tuberculosis Research and Epidemiology
- Antimicrobial Peptides and Activities
- COVID-19 diagnosis using AI
- Mycobacterium research and diagnosis
- HIV Research and Treatment
- Biochemical and Structural Characterization
- vaccines and immunoinformatics approaches
- AI in cancer detection
- Infectious Diseases and Tuberculosis
- Biomedical and Engineering Education
- Radiomics and Machine Learning in Medical Imaging
- Neonatal Health and Biochemistry
- Biochemical and Molecular Research
- Hormonal Regulation and Hypertension
- RNA and protein synthesis mechanisms
- 3D Printing in Biomedical Research
- Estrogen and related hormone effects
- Diagnosis and treatment of tuberculosis
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Malaria Research and Control
- HIV/AIDS drug development and treatment
- Anatomy and Medical Technology
- Biomedical Text Mining and Ontologies
- Adrenal Hormones and Disorders
National Institutes of Health
2015-2024
National Institute of Allergy and Infectious Diseases
2015-2024
Naval Research Laboratory Information Technology Division
2010-2016
Office of the National Coordinator for Health Information Technology
2010-2015
Howard Hughes Medical Institute
2015
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2010-2014
Cornell University
2006
The availability of nutrients influences cellular growth and survival by affecting gene transcription. Glucocorticoids also influence transcription have diverse activities on cell growth, energy expenditure, survival. We found that the arrest-specific 5 (Gas5) noncoding RNA, which is abundant in cells whose has been arrested because lack or factors, sensitized to apoptosis suppressing glucocorticoid-mediated induction several responsive genes, including one encoding inhibitor 2. Gas5 bound...
The Database of Antimicrobial Activity and Structure Peptides (DBAASP) is an open-access, comprehensive database containing information on amino acid sequences, chemical modifications, 3D structures, bioactivities toxicities peptides that possess antimicrobial properties. DBAASP updated continuously, at present, version 3.0 (DBAASP v3) contains >15 700 entries (8000 more than the previous version), including >14 500 monomers nearly 400 homo- hetero-multimers. Of monomeric (AMPs), >12 000 are...
Antimicrobial peptides (AMPs) are anti-infectives that may represent a novel and untapped class of biotherapeutics. Increasing interest in AMPs means new (natural synthetic) discovered faster than ever before. We describe herein version the Database Activity Structure Peptides (DBAASPv.2, which is freely accessible at http://dbaasp.org). This iteration database reports chemical structures empirically-determined activities (MICs, IC50, etc.) against more 4200 specific target microbes for 2000...
The Plasmodium falciparum circumsporozoite protein (CSP) is critical for sporozoite function and invasion of hepatocytes. Given its nature, a phase III human CSP malaria vaccine trial ongoing. composed three regions as follows: an N terminus that binds heparin sulfate proteoglycans, four amino acid repeat region (NANP), C contains thrombospondin-like type I (TSR) domain. Despite the importance CSP, little known about structure. Therefore, recombinant forms were produced by expression in both...
3D printing, also known as additive manufacturing, is the computer-guided process of fabricating physical objects by depositing successive layers material. It has transformed manufacturing across virtually every industry, bringing about incredible advances in research and medicine. The rapidly growing consumer market now includes convenient affordable "desktop" printers. These are being used laboratory to create custom 3D-printed equipment, a community designers contributing open-source,...
Abstract Context: Aldosterone production in the adrenal zona glomerulosa is mainly regulated by angiotensin II, [K+], and ACTH. Genetic deletion of subunits K+-selective leak (KCNK) channels TWIK-related acid sensitive K+-1 and/or K+-3 mice results primary hyperaldosteronism, whereas mutations KCNJ5 (potassium inwardly rectifying channel, subfamily J, member 5) gene are implicated hyperaldosteronism and, certain cases, autonomous cell proliferation humans. Objective: The objective study was...
Widespread interest in the study of microbiome has resulted data proliferation and development powerful computational tools. However, many scientific researchers lack time, training, or infrastructure to work with large datasets install use command line
ABSTRACT The TB Portals program is an international consortium of physicians, radiologists, and microbiologists from countries with a heavy burden drug-resistant tuberculosis working data scientists information technology professionals. Together, we have built the Portals, repository socioeconomic/geographic, clinical, laboratory, radiological, genomic patient cases backed by shareable, physical samples. Currently, there are 1,299 total five country sites (Azerbaijan, Belarus, Moldova,...
The advent of Next Generation Sequencing (NGS) technologies has opened new possibilities for researchers. However, the more biology becomes a data-intensive field, biologists have to learn how process and analyze NGS data with complex computational tools. Even availability common pipeline specifications, it is often time-consuming cumbersome task bench scientist install configure We believe that unified, desktop biologist-friendly front end analysis tools will substantially improve...
Antigenic drift in the influenza A virus hemagglutinin (HA) is responsible for seasonal reformulation of vaccines. Here, we address an important and largely overlooked issue antigenic drift: how does number location glycosylation sites affect HA evolution man? We analyzed status all full-length H1 subtype sequences available NCBI database. devised "flow index" (FI), a simple algorithm that calculates tendency viruses to gain or lose consensus sites. The FI predicts predominance states among...
The National Institutes of Health (NIH) has launched the NIH 3D Print Exchange, an online portal for discovering and creating bioscientifically relevant models suitable printing, to provide both researchers educators with a trusted source discover accurate informative models. There are number resources prints, but there is paucity scientific models, expertise required generate validate such remains barrier. Exchange fills this gap by providing novel, web-based tools that empower users...
Antimicrobial peptides (AMPs) have been identified as a potential new class of anti-infectives for drug development. There are lot computational methods that try to predict AMPs. Most them can only if peptide will show any antimicrobial potency, but the best our knowledge, there no tools which potency against particular strains. Here we present predictive model linear AMPs being active Gram-negative strains relying on semi-supervised machine-learning approach with density-based clustering...
Antimicrobial peptides (AMPs) have been identified as a potentially new class of antibiotics to combat bacterial resistance conventional drugs. The design de novo AMPs with high therapeutic indexes, low cost synthesis, proteases and bioavailability remains challenge. Such requires computational modeling antimicrobial properties. Currently, most methods cannot accurately calculate potency against particular strains pathogens. We developed tool for AMP prediction (Special Prediction (SP) tool)...
Epidemics and pandemics are causing high morbidity mortality on a still-evolving scale exemplified by the COVID-19 pandemic. Infection prevention control (IPC) training for frontline health workers is thus essential. However, classroom or hospital ward-based portends an infection risk due to in-person interaction of participants. We explored use Virtual Reality (VR) simulations worker since it trains participants without exposing them infections that would arise from training. It does away...
Membrane-associated dihydroorotate dehydrogenase (DHODH) is an antimalarial therapeutic target without effective inhibitor. Studies on human DHODH (HsDHODH) led to a structural mechanistic model in which respiratory quinones bind tunnel formed by the highly variable N-terminus that leads flavin mononucleotide-binding site. The agents leflunomide (Arava) and brequinar sodium inhibit HsDHODH binding this tunnel. Plasmodium falciparum (PfDHODH) have markedly different sensitivities two drugs....
Primary generalized glucocorticoid resistance is a rare genetic disorder characterized by generalized, partial, target-tissue insensitivity to glucocorticoids. The molecular basis of the condition has been ascribed inactivating mutations in human receptor (hGR) gene.The objective study was present three new cases caused novel mutation hGR gene and delineate mechanisms through which mutant impairs signal transduction.The index case (father) his two daughters presented with increased urinary...
Tuberculosis is a major global health threat claiming millions of lives each year. While the total number tuberculosis cases has been decreasing over last years, rise drug-resistant reduced chance controlling disease. The purpose to implement timely diagnosis tuberculosis, which essential administering adequate treatment regimens and stopping further transmission tuberculosis.A main tool for diagnosing conventional chest X-ray. We are investigating possibility discriminating automatically...
The evolution of drug-resistant pathogenic microbial species is a major global health concern. Naturally occurring, antimicrobial peptides (AMPs) are considered promising candidates to address antibiotic resistance problems. A variety computational methods have been developed accurately predict AMPs. majority such not strain specific (MSS): they can whether given peptide active against some microbe, but cannot calculate would be particular MS. Due insufficient data on most MS, only few MSS...
Antimicrobial peptides (AMPs) have emerged as promising candidates in combating antimicrobial resistance – a growing issue healthcare. However, to develop AMPs into effective therapeutics, thorough analysis and extensive investigations are essential. In this study, we employed an silico approach design cationic de novo, followed by their experimental testing. The antibacterial potential of novo designed AMPs, along with synergistic properties combination conventional antibiotics was...
Context: Generalized glucocorticoid resistance syndrome is a rare familial or sporadic condition characterized by partial insensitivity to glucocorticoids, caused mutations in the receptor (GR) gene. Most of reported cases are adults, demonstrating symptoms associated with mineralocorticoid and/or adrenal androgen excess compensatively increased secretion adrenocorticotropic hormone.
Primary generalized glucocorticoid resistance is a rare genetic condition characterized by partial end-organ insensitivity to glucocorticoids. Most affected subjects present with clinical manifestations of mineralocorticoid and androgen excess. The has been associated inactivating mutations in the human receptor (hGR) gene, which impair molecular mechanisms hGRα action, thereby reducing tissue sensitivity glucocorticoids.ΤHE aim our study was investigate through one previously described...
Availability of trained radiologists for fast processing CXRs in regions burdened with tuberculosis always has been a challenge, affecting both timely diagnosis and patient monitoring. The paucity annotated images lungs TB patients hampers attempts to apply data-oriented algorithms research clinical practices. Portals Program database (TBPP, https://TBPortals.niaid.nih.gov) is global collaboration curating large collection the most dangerous, hard-to-cure drug-resistant (DR-TB) cases. TBPP,...