A5031554107- Zebrafish Biomedical Research Applications
- PI3K/AKT/mTOR signaling in cancer
- Angiogenesis and VEGF in Cancer
- Hippo pathway signaling and YAP/TAZ
- Advanced biosensing and bioanalysis techniques
- interferon and immune responses
- Metabolism, Diabetes, and Cancer
- Cancer-related Molecular Pathways
- Protein Kinase Regulation and GTPase Signaling
- Tuberous Sclerosis Complex Research
- Mast cells and histamine
- Congenital heart defects research
- Polyamine Metabolism and Applications
- Mechanisms of cancer metastasis
- Viral Infectious Diseases and Gene Expression in Insects
- Pancreatic function and diabetes
- Peptidase Inhibition and Analysis
- Histiocytic Disorders and Treatments
- Nanofabrication and Lithography Techniques
- Monoclonal and Polyclonal Antibodies Research
- Endoplasmic Reticulum Stress and Disease
- Advanced Proteomics Techniques and Applications
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
University of Basel
2010-2023
Brigham and Women's Hospital
2009
The target of rapamycin (TOR) is a highly conserved protein kinase and central controller growth. Mammalian TOR complex 2 (mTORC2) regulates AGC family members implicated in various disorders, including cancer diabetes. Here we report that mTORC2 localized to the endoplasmic reticulum (ER) subcompartment termed mitochondria-associated ER membrane (MAM). localization MAM was growth factor-stimulated, at interacted with IP 3 receptor (IP3R)-Grp75–voltage-dependent anion-selective channel 1...
During embryonic development, vascular networks remodel to meet the increasing demand of growing tissues for oxygen and nutrients. This is achieved by pruning redundant blood vessel segments, which then allows more efficient flow patterns. Because lack an in vivo system suitable high-resolution live imaging, dynamics process have not been described detail. Here, we present subintestinal vein (SIV) plexus zebrafish embryo as a novel model study at cellular level. We show that regression...
Synthetic lethality between the clinically approved noncancer drugs metformin and syrosingopine specifically kills cancer cells.
ABSTRACT Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish accessible modular system. Here, we establish several next-generation tools and other species to expand enhance transgenic applications. To facilitate gene regulatory element testing, generated Gateway middle entry vectors harboring the small mouse beta-globin minimal promoter coupled fluorophores, CreERT2 Gal4. extend...
Here, we present the full integration of a proximity ligation assay (PLA) on microfluidic chip for systematic cell signaling studies. PLA is an in situ technology detection protein interaction, post-translational modification, concentration, and cellular location with single-molecule resolution. Analytical performance advances are achieved, including automation biochemical steps, target multiplexing, reduction antibody consumption by 2 orders magnitude relative to standard procedures. In...
Tuberous sclerosis complex (TSC) is a tumor suppressor gene syndrome in which hamartomas develop multiple organ systems. Knockout and conditional alleles of Tsc1 Tsc2 have been previously reported. Here, we describe the generation novel hypomorphic allele (del3), exon 3, encoding 37 amino acids near N terminus tuberin, deleted. Embryos homozygous for del3 survive until E13.5, 2 days longer than null embryos. die from underdevelopment liver, deficient hematopoiesis, aberrant vascular...
Organ morphogenesis is driven by a wealth of tightly orchestrated cellular behaviors, which ensure proper organ assembly and function. Many these cell activities involve cell-cell interactions remodeling the F-actin cytoskeleton. Here, we analyze requirement for Rasip1 (Ras-interacting protein 1), an endothelial-specific regulator junctional dynamics, during blood vessel formation. Phenotype analysis rasip1 mutants in zebrafish embryos reveals distinct functions sprouting angiogenesis,...
Abstract Organ morphogenesis is driven by a wealth of tightly orchestrated cellular behaviors, which ensure proper organ assembly and function. Many these cell activities involve cell-cell interactions remodeling the F-actin cytoskeleton. Here, we analyze requirement for Rasip1 (Ras-interacting protein 1), an endothelial-specific regulator junctional dynamics, during blood vessel formation. Phenotype analysis rasip1 mutants in zebrafish embryos reveal distinct requirements sprouting...
Abstract Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish accessible, modular system. Here, we established several next-generation tools and other species to expand enhance transgenic applications. To facilitate gene-regulatory element testing, generated Gateway middle entry vectors harboring the small mouse beta-globin minimal promoter coupled fluorophores, CreERT2, Gal4. extend...