A5083314637- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Force Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Plant biochemistry and biosynthesis
- Micro and Nano Robotics
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Biocrusts and Microbial Ecology
- Biochemical and biochemical processes
- Molecular Biology Techniques and Applications
- Orbital Angular Momentum in Optics
- Pickering emulsions and particle stabilization
- Mechanical and Optical Resonators
- GABA and Rice Research
- Plant Stress Responses and Tolerance
- Phytochemicals and Medicinal Plants
- Journalism and Media Studies
- Bacteriophages and microbial interactions
University of California, Berkeley
2018-2024
Stanford University
2024
QB3
2022-2023
Howard Hughes Medical Institute
2022-2023
Pontifical Catholic University of Peru
2018-2021
In prokaryotes, translation can occur on mRNA that is being transcribed in a process called coupling. How the ribosome affects RNA polymerase (RNAP) during coupling not well understood. Here, we reconstituted E. coli system and demonstrated prevent pausing termination of RNAP double overall transcription rate at expense fidelity. Moreover, monitored single RNAPs coupled to ribosomes show increases pause-free velocity mechanical assisting force sufficient explain majority effects Also, by...
It has been proposed that the intrinsic property of nucleosome arrays to undergo liquid-liquid phase separation (LLPS) in vitro is responsible for chromatin domain organization vivo. However, understanding nucleosomal LLPS hindered by challenge characterize structure resulting heterogeneous condensates. We used cryo-electron tomography and deep-learning-based 3D reconstruction/segmentation determine molecular condensates at various stages LLPS. show involves a two-step process: spinodal...
Nucleosome DNA unwrapping and its disassembly into hexasomes tetrasomes is necessary for genomic access plays an important role in transcription regulation. Previous single-molecule mechanical nucleosome revealed a low- high-force transitions, force-FRET pulling experiments showed that asymmetric, occurring always first from one side before the other. However, assignment of segments involved these transitions remains controversial. Here, using high-resolution optical tweezers with...
Significance Optical tweezers enable scientists to follow the dynamics of molecular motors at high resolution. The ability discern a motor’s discrete steps reveals important insights on its operation. Some operate scale angstroms, rendering observation their extremely challenging. In some cases, such small have been observed sporadically; however, full trajectories and intervals between remain elusive due instrumental noise. Here, we eliminate main source noise most high-resolution dual-trap...
During replication, expression, and repair of the eukaryotic genome, cellular machinery must access DNA wrapped around histone proteins forming nucleosomes. These octameric protein·DNA complexes are modular, dynamic, flexible unwrap or disassemble either spontaneously by action molecular motors. Thus, mechanism formation regulation subnucleosomal intermediates has gained attention genome-wide because it controls accessibility. Here, we imaged nucleosomes their more compacted structure with...
Abstract Nucleosome DNA unwrapping and its disassembly into hexasomes tetrasomes is necessary for genomic access plays an important role in transcription regulation. Previous single-molecule mechanical nucleosome revealed a low- high-force transitions, force-FRET pulling experiments showed that asymmetrical occurring always first from one side before the other. However, assignment of segments involved these transitions remains controversial. Here, using high-resolution optical tweezers with...
A diferencia de los materiales inertes en equilibrio, la materia activa como organismos biológicos se encuentran fuera del equilibrio generando movimiento y ensamblajes a partir interacciones moleculares reacciones químicas nano- microescala. Anteriormente, estas propiedades consideraban exclusivas vivos; sin embargo, avances ciencia coloides han permitido generar análogos sintéticos conocidos activos que emulan dichas propiedades. En este artículo, realiza primero una introducción las...
Summary It has been proposed that the intrinsic property of nucleosome arrays to undergo liquid-liquid phase separation (LLPS) in vitro is responsible for chromatin domain organization vivo . However, understanding nucleosomal LLPS hindered by challenge characterize structure resulting heterogeneous condensates. We used cryo-electron tomography and deep learning-based 3D reconstruction/segmentation determine molecular condensates at various stages LLPS. show involves a two-step process:...