A5015749025- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- Plant Pathogens and Fungal Diseases
- Metabolomics and Mass Spectrometry Studies
- Plant Gene Expression Analysis
- Bioinformatics and Genomic Networks
- Plant biochemistry and biosynthesis
- GABA and Rice Research
- Seed Germination and Physiology
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Bacterial biofilms and quorum sensing
- Plant Genetic and Mutation Studies
- Potato Plant Research
- Bacterial Genetics and Biotechnology
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Computational Drug Discovery Methods
- Plant Physiology and Cultivation Studies
- Natural Products and Biological Research
- Postharvest Quality and Shelf Life Management
- Plant nutrient uptake and metabolism
- Sensory Analysis and Statistical Methods
Ben-Gurion University of the Negev
2012-2023
Pontifical Catholic University of Peru
2018-2023
Tel Aviv University
2022
University of California, Davis
2018-2020
Plant (United States)
2018-2020
Max Planck Institute of Molecular Plant Physiology
2012
Abstract Background Grapevine metabolism in response to water deficit was studied two cultivars, Shiraz and Cabernet Sauvignon, which were shown have different hydraulic behaviors (Hochberg et al. Physiol. Plant . 147:443–453, 2012). Results Progressive found effect changes leaf potentials accompanied by metabolic changes. In both but more intensively than caused a shift higher osmolality lower C/N ratios, the latter of also reflected marked increases amino acids, e.g., Pro, Val, Leu, Thr...
To investigate the regulation of seed metabolism and to estimate degree metabolic natural variability, metabolite profiling network analysis were applied a collection 76 different homozygous tomato introgression lines (ILs) grown in field two consecutive harvest seasons. Factorial ANOVA confirmed presence 30 quantitative trait loci (mQTL). Amino acid contents displayed high variability across population, with similar patterns seasons, while sugars exhibited significant seasonal fluctuations....
In the last decade vast data sets are being generated in biological and medical studies. The challenge lies their summary, complexity reduction, interpretation. Correlation-based networks graph-theory based properties of this type can be successfully used during process. However, procedure has its pitfalls requires specific knowledge that often lays beyond classical biology includes many computational tools software. Here we introduce one a series methods for correlation-based network...
Abstract The identification and understanding of metabolic pathways is a key aspect in crop improvement drug design. common approach for their detection based on gene annotation ontology. Correlation-based network analysis, where metabolites are arranged into formation, used as complentary tool. Here, we demonstrate the correlation-based analysis combined with machine-learning techniques. Metabolites known tomato pathways, non-tomato random sets were mapped subgraphs onto metabolite...
Wine grapes are important economically in many countries around the world. Defining optimum time for grape harvest is a major challenge to grower and winemaker. Berry skins an source of flavor, color other quality traits ripening stage. Senescent-like processes such as chloroplast disorganization cell death characterize late To better understand molecular physiological involved stages berry ripening, RNA-seq analysis seven wine cultivars (Cabernet Franc, Cabernet Sauvignon, Merlot, Pinot...
Summary Correlation‐based network analysis (CNA) of the metabolic profiles seeds a tomato introgression line mapping population revealed clique proteinogenic amino acids: Gly, Ile, Pro, Ser, Thr, and Val. Correlations between these acids exhibited statistically significant average correlation coefficient 0.84 as compared with an 0.39 over 16 119 other metabolite cliques containing six metabolites. In silico removal was used to quantify their importance in determining seminal properties,...
Summary The micropylar region of endosperm in a seed, which is adjacent to the radicle tip, called ‘endosperm cap’, and specifically activated before emergence. This activation cap widespread phenomenon among species prerequisite for completion germination. To understand mechanisms cap‐specific gene expression tomato seeds, GeneChip analysis was performed. major groups cap‐enriched genes were pathogenesis‐, cell wall‐, hormone‐associated genes. promoter regions contained DNA motifs...
The metabolite content of a seed and its ability to germinate are determined by genetic makeup environmental effects during development. interaction between genetics, environment metabolism germination was studied in 72 tomato homozygous introgression lines (IL) derived from Solanum pennelli S. esculentum M82 cultivar. Plants were grown the field under saline fresh water irrigation two consecutive seasons, collected seeds subjected morphological analysis, gas chromatograph-mass spectrometry...
Production of public goods in biological systems is often a collaborative effort that may be detrimental to the producers. It therefore sustainable only if small fraction population shoulders cost while majority reap benefits. We modelled this scenario using Escherichia coli populations producing colicins, an antibiotic kills producer cells' close relatives. Colicin expression costly trait, and it has been proposed actively expresses antibiotic. Colicinogenic were followed at single-cell...
To investigate the natural variability of leaf metabolism and enzymatic activity in a maize inbred population, statistical network analyses were employed on metabolite enzyme profiles. The test coefficient variation showed that sugars amino acids displayed opposite trends their variance within consistently with related enzymes. overall higher CV values for metabolites as compared to tested enzymes are indicative greater phenotypic plasticity. H2 tests revealed galactinol (1) asparagine...
Abstract Perennial grasses will account for approximately 16 billion gallons of renewable fuels by the year 2022, contributing significantly to carbon and nitrogen sequestration. However, perennial productivity can be limited severe freezing conditions in some geographical areas, although these risks could decrease with advance climate warming, possibility unpredictable early cold events cannot discarded. We conducted a study on model grass Brachypodium sylvaticum investigate molecular...
Changes in climate conditions can negatively affect the productivity of crop plants. They induce chloroplast degradation (senescence), which leads to decreased source capacity, as well whole-plant carbon/nitrogen assimilation and allocation. The importance, contribution mechanisms action regulating source-tissue capacity under stress tomato (Solanum lycopersicum) are not understood. We hypothesized that delaying by altering activity vesiculation (CV) would lead more efficient use carbon...
Weighted gene co-expression network analysis (WGCNA) is a widely used software tool that to establish relationships between phenotypic traits and expression data. It generates modules then correlates their first principal component traits, proposing functional relationship expressed by the correlation coefficient. However, often contain thousands of genes different backgrounds. Here, we developed stochastic optimization algorithm, known as genetic algorithm (GA), optimizing trait module...
Abstract Background Correlation network analysis has become an integral tool to study metabolite datasets. Networks are constructed by omitting correlations between metabolites based on two thresholds—namely the r and associated p -values. While -value threshold settings follow rules of multiple hypotheses testing correction, guidelines for have not been defined. Results Here, we introduce a method that allows determining iterative approach, where different networks their topology is...
The enzyme 1-amino-cyclopropane-1-carboxylic acid synthase (ACS) participates in the ethylene biosynthesis pathways and it is tightly regulated transcriptionally post-translationally. Notwithstanding its major role climacteric fruit ripening, transcriptional regulation of ACS during ripening not fully understood. We studied two Japanese plum cultivars, Santa Rosa (SR) non-climacteric bud sport mutant, Sweet Miriam (SM). As cultivars show considerable difference expression, they provide a...
Many studies have described the response of facultative anaerobe, Escherichia coli, to anaerobic conditions, yet they all investigated free-living (planktonic) cells because attempts cultivate E. coli biofilm were mostly unsuccessful. We challenged these findings and cultivated strain MG1655 under both aerobic characterizing mature architecture global gene expression profile. used RNA sequencing technology compare stationary phase planktonic with biofilm, cultured without oxygen. Our results...