A5053265776- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Bacteriophages and microbial interactions
- Ion Channels and Receptors
- Advanced biosensing and bioanalysis techniques
- Cardiac electrophysiology and arrhythmias
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neural Engineering
- Cellular transport and secretion
- Bacterial Genetics and Biotechnology
- Photoreceptor and optogenetics research
- Indigenous Health and Education
- Gut microbiota and health
- Genomics and Chromatin Dynamics
- DNA and Nucleic Acid Chemistry
- Complement system in diseases
- Psychological Treatments and Disorders
- Drug Solubulity and Delivery Systems
- Lipid Membrane Structure and Behavior
- Criminal Justice and Penology
- Neuroscience and Neuropharmacology Research
- Philosophical and Cultural Analysis
- Protist diversity and phylogeny
- Membrane-based Ion Separation Techniques
- Lysosomal Storage Disorders Research
University of Valparaíso
2011-2025
Alberto Hurtado University
2023
University of California, Berkeley
2018-2022
Valparaiso University
2014-2022
QB3
2020-2022
Universidad de Santiago de Chile
2022
Casma Therapeutics (United States)
2022
University of Chile
2011-2019
Hero España (Spain)
2019
Howard Hughes Medical Institute
2018
Abstract The Na + /K -ATPase restores sodium (Na ) and potassium (K electrochemical gradients dissipated by action potentials ion-coupled transport processes. As ions are transported, they become transiently trapped between intracellular extracellular gates. Once the external gate opens, three released, followed binding occlusion of two K ions. While mechanisms release have been well characterized study transient currents, smaller faster currents mediated more difficult to study. Here we...
Nucleosome DNA unwrapping and its disassembly into hexasomes tetrasomes is necessary for genomic access plays an important role in transcription regulation. Previous single-molecule mechanical nucleosome revealed a low- high-force transitions, force-FRET pulling experiments showed that asymmetric, occurring always first from one side before the other. However, assignment of segments involved these transitions remains controversial. Here, using high-resolution optical tweezers with...
The endosomal sorting complexes required for transport (ESCRT) system is an ancient and ubiquitous membrane scission machinery that catalyzes the budding of membranes. ESCRT-mediated events, exemplified by those involved in HIV-1, are usually directed away from cytosol ("reverse topology"), but they can also be toward ("normal topology"). ESCRT-III subunits CHMP1B IST1 coat constrict positively curved tubes, suggesting these could catalyze normal topology severing. bind recruit...
The knowledge of the host-guest complexes using cyclodextrins (CDs) has prompted an increase in development new formulations. capacity these organic host structures including guest within their hydrophobic cavities, improves physicochemical properties guest. In case pesticides, several inclusion with have been reported. However, order to explore rationally pesticide formulations, it is essential know effect on molecules. this study, bentazon (Btz) native βCD and two derivatives,...
ABSTRACT The molecular and physical communication within the microscopic world underpins entire web of life as we know it. However, how organisms, such bacteria, amoebae, nematodes—all ubiquitous—interact to sustain their ecological niches, particularly associations generate influence behavior, remains largely unknown. In this study, developed a framework examine long-term interactions between microbes animals. From soil samples collected in temperate, semi-arid climate, isolated culturable...
Significance The φ29 DNA packaging motor can be thought of as a five-cylinder engine, where each cylinder (subunit) catalyzes ATP hydrolysis to power translocation. operates in two phases: first, the subunits load fuel (ATP) sequentially one at time; second, all rapidly hydrolyze sequential fashion. How coordinate their activities remained unknown. Here, we show that use regulatory features observed signaling pathways, very different biological context, activities. Moreover, is stimulated by...
Bacterivore nematodes are the most abundant animals in biosphere, largely contributing to global biogeochemistry. Thus, effects of environmental microbes on nematodes' life-history traits likely contribute general health biosphere. Caenorhabditis elegans is an excellent model study behavioral and physiological outputs microbial diets. However, complex natural bacterial assemblies have only recently been reported, as studies carried out with monoxenic cultures laboratory-reared bacteria....
The Na + /K pump is a nearly ubiquitous membrane protein in animal cells that uses the free energy of ATP hydrolysis to alternatively export 3Na from cell and import 2K per cycle. This exchange ions produces steady-state outwardly directed current, which proportional magnitude turnover rate. Under certain ionic conditions, sudden voltage jump generates temporally distinct transient currents mediated by represent kinetics extracellular binding/release occlusion/deocclusion transitions. For...
Mammals maintain homeostatic control of their body temperature. Therefore, these organisms are expected to have adaptations that confer the ability detect and react both self ambient Temperature-activated ion channels been discovered be primary molecular determinants thermosensation. The most representative group constitutes members transient receptor potential superfamily, TRP, which activated by either low or high temperatures covering whole range physiologically relevant temperatures....
Significance Large-conductance Ca 2+ - and voltage-activated K + (BK) channels play many physiological roles, ranging from the maintenance of smooth muscle tone to modulation alcohol tolerance. In most cases, this versatility BK channel is due association pore-forming α-subunit with β-subunits. Therefore, it importance know what structural consequences are. Here, using lanthanide-based resonance energy transfer, we were able determine extracellular position transmembrane segments S0–S2...
Ring ATPases that translocate disordered polymers possess lock-washer architectures they impose on their substrates during transport via a hand-over-hand mechanism. Here, we investigate the operation of ring motors ordered, helical substrates, such as bacteriophage ϕ29 dsDNA packaging motor. This pentameric motor alternates between an ATP loading dwell and hydrolysis burst wherein it packages one turn DNA in four steps. When challenged with DNA-RNA hybrids dsRNA, matches its to shorter...
Abstract The endosomal sorting complexes required for transport (ESCRT) system is an ancient and ubiquitous membrane scission machinery that catalyzes the budding of membranes. ESCRT-mediated events, exemplified by those involved in HIV-1, are usually directed away from cytosol (‘reverse-topology’), but they can also be towards (‘normal-topology’). Of ESCRT 0-III, ESCRT-III most directly implicated severing. Various subunits recruit AAA + ATPase VPS4, which essential disassembly reverse...
Abstract Nucleosome DNA unwrapping and its disassembly into hexasomes tetrasomes is necessary for genomic access plays an important role in transcription regulation. Previous single-molecule mechanical nucleosome revealed a low- high-force transitions, force-FRET pulling experiments showed that asymmetrical occurring always first from one side before the other. However, assignment of segments involved these transitions remains controversial. Here, using high-resolution optical tweezers with...
Ring ATPases that translocate disordered polymers possess lock-washer architectures they impose on their substrates during transport via a hand-over-hand mechanism. Here, we investigate the operation of ring motors possessing preexisting helical structure, such as bacteriophage ϕ29 dsDNA packaging motor. During each cycle, this pentameric motor tracks one helix strand (the ‘tracking strand’), and alternates between two segregated phases: dwell in which it exchanges ADP for ATP burst packages...
Abstract The molecular and physical communication within the microworld supports entire web of life as we know it. How organisms such bacteria, amoeba nematodes -all superabundant-interact to sustain their niche, however, is not known, especially how associations generate affect behavior animals in fluctuating environments. To have a frame study interactions between microbe animal, collected soil from temperate semi-arid climate isolated culturable genus bacteria Comamonas, Stenotrophomonas,...