A5024838265- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Plant Molecular Biology Research
- Genetics, Aging, and Longevity in Model Organisms
- Spectroscopy and Chemometric Analyses
- RNA Research and Splicing
- Advanced Chemical Sensor Technologies
- Plant Virus Research Studies
- Analytical Chemistry and Chromatography
- Plant Reproductive Biology
- Diet and metabolism studies
- Tryptophan and brain disorders
- RNA regulation and disease
- Weed Control and Herbicide Applications
- RNA and protein synthesis mechanisms
- Medical research and treatments
- NMR spectroscopy and applications
- Plant and soil sciences
- GABA and Rice Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Cellular transport and secretion
- Pesticide and Herbicide Environmental Studies
- Cell Adhesion Molecules Research
- Circadian rhythm and melatonin
- Endoplasmic Reticulum Stress and Disease
Instituto de Biología Molecular y Celular de Rosario
2010-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2024
National University of Rosario
2010-2024
Instituto Multidisciplinario de Biología Celular
2021-2024
Imperial College London
2017
Gut-microbiota membership is associated with diverse neuropsychological outcomes, including substance use disorders (SUDs). Here, we mice colonized Citrobacter rodentium or the human γ-Proteobacteria commensal Escherichia coli HS as a model to examine mechanistic interactions between gut microbes and host responses cocaine. We find that cocaine exposure increases intestinal norepinephrine levels are sensed through bacterial adrenergic receptor QseC promote colonization of γ-Proteobacteria....
Caenorhabditis elegans and its cognate bacterial diet comprise a reliable, widespread model to study microbiota effects on host physiology. Nonetheless, how influences the rate at which neurons die remains largely unknown. A number of models have been used in C. as surrogates for neurodegeneration. One these is strain expressing neurotoxic allele mechanosensory abnormality protein 4 (MEC-4d) degenerin/epithelial Na+ (DEG/ENaC) channel, causes progressive degeneration touch receptor (TRNs)....
A major purpose of exploratory metabolic profiling is for the identification molecular species that are statistically associated with specific biological or medical outcomes; unfortunately, structure elucidation process unknowns often a bottleneck in this process. We present here new holistic strategies combine different statistical spectroscopic and analytical techniques to improve simplify metabolite identification. exemplify these using study data collected as part dietary intervention...
HYL1 is a double-stranded RNA binding protein involved in microRNA processing plants. enhances the efficiency and precision of RNase III DCL1 participates strand selection. In this work, we dissect contributions domains to targets. We found that first domain main contributor binding. Mapping interaction regions by nuclear magnetic resonance on structure RNA-binding showed difference capabilities can be traced sequence divergence β2−β3 loop. The possible role each discussed light previous...
Bacterivore nematodes are the most abundant animals in biosphere, largely contributing to global biogeochemistry. Thus, effects of environmental microbes on nematodes' life-history traits likely contribute general health biosphere. Caenorhabditis elegans is an excellent model study behavioral and physiological outputs microbial diets. However, complex natural bacterial assemblies have only recently been reported, as studies carried out with monoxenic cultures laboratory-reared bacteria....
DCL1 is the ribonuclease that carries out miRNA biogenesis in plants. The enzyme has two tandem double stranded RNA binding domains (dsRBDs) its C-terminus. Here we show first of these binds precursor fragments when isolated and cooperates with second domain recognition substrate RNA. Remarkably, despite showing activity, this intrinsically disordered. We found it acquires a folded conformation bound to substrate, being report complete dsRBD folding upon binding. free unfolded form shows...
Dicer-like ribonuclease III enzymes are involved in different paths related to RNA silencing plants. Little is known about the structural aspects of these processes. Here we present a characterization second double-stranded binding domain (dsRBD) DCL1, which presumed participate pri-micro-RNA recognition and subcellular localization this protein. We determined solution structure found that it has canonical fold but bears some variation with respect other homologous domains. also binds both...
The identification of metabolites in complex biological matrices is a challenging task 1D 1H-NMR-based metabolomics studies. Statistical total correlation spectroscopy (STOCSY) has emerged for aiding the structural elucidation by revealing peaks that present high to driver peak interest (which would likely belong same molecule). However, these studies, signals from are normally as mixture overlapping resonances, limiting performance STOCSY. As an alternative avoid overlap issue, 2D 1H...
NMR-based metabolomics requires proper identification of metabolites to draw conclusions from the system under study. Normally, multivariate data analysis is performed using 1D 1H NMR spectra, and peaks (and then compounds) relevant classification accomplished database queries as a first step. spectra complex mixtures often suffer peak overlap. To overcome this issue, several studies employed projections (tilted symmetrized) 2D J-resolved (JRES) p-JRES, which are similar decoupled spectra....
MicroRNAs have turned out to be important regulators of gene expression. These molecules originate from longer transcripts that are processed by ribonuclease III (RNAse III) enzymes. Dicer proteins essential RNAse enzymes involved in the generation microRNAs (miRNAs) and other small RNAs. The correct function relies on participation accessory dsRNA binding proteins, exact which is not well-understood so far. In plants, double stranded RNA protein Hyponastic Leaves 1 (HYL1) helps Like (DCL1)...
NMR-based metabolomics aims at recovering biological information by comparing spectral data from samples of interest and appropriate controls. Any statistical analysis performed on the matrix relies proper peak alignment to produce meaningful results. Through last decades, several algorithms have been proposed, as well alternatives like binning or strategies for annotation quantification, latter depending reference databases. Most algorithms, mainly based segmentation spectra, present...
Abstract Caenorhabditis elegans and its cognate bacterial diet comprise a reliable, widespread model to study microbiota effects on host physiology. Nonetheless, how influences the rate at which neurons die remains largely unknown. A number of models have been used in C. as surrogates for neurodegeneration. One these is strain expressing neurotoxic allele MEC-4(d) DEG/ENaC channel causes progressive degeneration touch receptor (TRNs). Using such model, this evaluated effect various dietary...
Abstract Bacterivore nematodes are the most abundant animals in biosphere, largely contributing to global biogeochemistry. The effect of environmental microbes as source associated microbiota and natural diet on their life history traits is likely impact general health biosphere. Caenorhabditis elegans a unique model study behavioral physiological outputs different available microbial diets. Nonetheless, studies monoaxenic cultures laboratory bacteria while isolates has only recently started...