A5059654983- Tuberculosis Research and Epidemiology
- Congenital heart defects research
- Diagnosis and treatment of tuberculosis
- Biomedical Text Mining and Ontologies
- Infectious Diseases and Tuberculosis
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- COVID-19 diagnosis using AI
- Estrogen and related hormone effects
- Blood groups and transfusion
- Research Data Management Practices
- Genetics, Aging, and Longevity in Model Organisms
- T-cell and B-cell Immunology
- Congenital Heart Disease Studies
- Genetic Associations and Epidemiology
- Animal Genetics and Reproduction
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Radiomics and Machine Learning in Medical Imaging
- Cancer Genomics and Diagnostics
- Genomics and Phylogenetic Studies
- Cell Image Analysis Techniques
National Institute of Allergy and Infectious Diseases
2017-2024
National Institutes of Health
2021-2024
National Cancer Institute
2014
Cornell University
2009-2013
Albert Einstein College of Medicine
2007-2009
Abstract Objective Autoimmune diseases predominantly affect women, suggesting that female sex hormones may play a role in the pathogenesis of such diseases. We have previously shown persistent mild‐to‐moderate elevations serum prolactin levels induce break self tolerance mice with BALB/c genetic background. The aim this study was to evaluate effects hyperprolactinemia on mechanisms B cell induction. Methods Effects splenic subsets were studied mice. receptor (BCR)–mediated apoptosis and...
Cardiac morphogenesis is a complex multi-stage process, and the molecular basis for controlling distinct steps remains poorly understood. Because gata4 encodes key transcriptional regulator of morphogenesis, we profiled transcript changes in cardiomyocytes when Gata4 protein depleted from developing zebrafish embryos. We discovered that regulates expression two small heat shock genes, hspb7 hspb12, both which are expressed embryonic heart. show depletion Hspb7 or Hspb12 disrupts normal...
ABSTRACT Genetic simulation programs are used to model data under specified assumptions facilitate the understanding and study of complex genetic systems. Standardized sets generated using essential for development application novel analytical tools in epidemiology studies. With continuing advances high‐throughput genomic technologies generation analysis larger, more sets, there is a need updating current approaches modeling. To provide forum address emerging challenges this area, National...
Objective. Estrogen has been found to exacerbate disease activity in murine lupus and induce a lupus-like syndrome nonspontaneously autoimmune mice. This led the consideration that estrogen may be risk factor for development of systemic erythematosus (SLE), selective receptor modulators (SERM) serve ameliorate activity. We evaluated effects mechanism action SERM raloxifene lupus. Methods. Effects on NZB/W F1 mice were presence absence by assessing serum DNA reactivity, glomerular IgG...
The TB Portals program provides a publicly accessible repository of case data containing multi-modal information such as clinical characteristics, pathogen genomics, and radiomics. real-world resource contains over 3400 cases, primarily drug resistant CT images with radiologist annotations are available for many these cases. breadth collected offers patient-centric view into the etiology disease including temporal context imaging information. Here, we analyze cohort new cases observations...
Radiology may better define tuberculosis (TB) severity and guide duration of treatment. We aimed to systematically study baseline chest X-rays (CXR) their association with TB treatment outcome using real-world data. used logistic regression associate outcomes CXR findings, including percent lung involved in disease (PLI), cavitation, Timika score, alone or combination other clinical characteristics, stratifying by drug resistance status HIV (n = 2,809). fine-tuned convolutional neural nets...
Gene function during embryogenesis is typically defined by loss-of-function experiments, for example targeted mutagenesis (knockout) in the mouse. In zebrafish model, effective reverse genetic techniques have been developed using microinjection of gene-specific antisense morpholinos. Morpholinos target an mRNA through specific base-pairing and block gene transiently inhibiting translation or splicing several days (knockdown). However, vertebrates such as mouse zebrafish, some functions can...
Abstract The Tuberculosis (TB) Portals is an international program of 14 countries connecting clinical, genomic, and radiologist specialists to develop openly available repository deidentified TB cases with multi-modal data such as case clinical characteristics, pathogen genomics, radiomics. This real-world resource contains over 4000 cases, principally drug resistant chest X-rays (CXR) images. scope curated offers a case-focused perspective into the drivers disease incorporating...
This study compares performance of Timika Score to standardized, detailed radiologist observations Chest X rays (CXR) for predicting early infectiousness and subsequent treatment outcome in drug sensitive (DS) or multi-drug resistant (MDR) tuberculosis cases. It seeks improvement prediction these clinical events through additional observations.This is a retrospective analyzing cases from the NIH/NIAID supported TB Portals database, large, trans-national, multi-site cohort primarily...
Abstract While the biomedical community has embraced data sharing (e.g. results, raw data) and supported establishment of large research consortia Human Microbiome Project) aimed to standardize quality important sets microbiome sequencing data, reusability most is still limited by its associated metadata. To ensure that indeed FAIR (Findable, Accessible, Interoperable, Reusable), it necessary consider tools approaches make easier provide high-quality metadata fit for purpose moving forward....
<h3>Background</h3> Autoreactive lymphocytes are constantly generated and eliminated to maintain tolerance. Two major mechanisms for tolerance induction deletion receptor editing. In the spleen, majority of negative selection occurs in transitional B cells, mainly T1 B-cell subset, resulting a T1:T2 ratio > 1. We have shown that prolactin-altered autoreactive specificities allows survival activation DNA-reactive cells development lupus mice do not develop spontaneously. BALB/c mice,...
Abstract While the impact of biomedical research has traditionally been measured using bibliographic metrics such as citation or journal factor, data itself is an output which can be directly to provide additional context about a publication’s impact. Data are resource that repurposed and reused providing dividends on original investment used support primary work. Moreover, it cornerstone upon tested hypothesis rejected accepted specific scientific conclusions reached. Understanding how...
Restricted accessAbstractFirst published March 2007Prolactin Alters Bcr-Mediated Apoptosis and Receptor EditingE. Peeva, G.E. Rosenfeld, […], J. Gonzalez, S. Saha+1-1View all authors affiliationsVolume 55, Issue 2https://doi.org/10.1177/108155890705500256
ABSTRACT BACKGROUND Drug-resistant (DR) tuberculosis treatment is challenging and frequently leads to poor outcomes. An international collaboration, the National Institute of Allergy Infectious Diseases (NIAID) TB Portals develops, maintains, supports a multi-national database cases, with an emphasis on drug-resistant tuberculosis. Patient records include clinical, radiological, genomic, socioeconomic features. Establishing factors associated unsuccessful may help optimize for most...