A5102928305- Veterinary Oncology Research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Virus-based gene therapy research
- Veterinary Medicine and Surgery
- Cancer Genomics and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Hematopoietic Stem Cell Transplantation
- Organ Transplantation Techniques and Outcomes
- Cancer Mechanisms and Therapy
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Microbial infections and disease research
- Salivary Gland Tumors Diagnosis and Treatment
- Urological Disorders and Treatments
- 3D Printing in Biomedical Research
- Cancer-related Molecular Pathways
- Organ and Tissue Transplantation Research
- Microplastics and Plastic Pollution
- Bone Tumor Diagnosis and Treatments
- Hernia repair and management
- Hepatocellular Carcinoma Treatment and Prognosis
- Chromium effects and bioremediation
Hokkaido University Hospital
2017-2025
Rakuno Gakuen University
2023-2024
Hokkaido University
2017-2021
Azabu University
2011-2014
Kobe Steel (Japan)
1996-1997
Abstract Immunotherapy targeting immune checkpoint molecules, programmed cell death 1 (PD-1) and PD-ligand (PD-L1), using therapeutic antibodies has been widely used for some human malignancies in the last 5 years. A costimulatory receptor, PD-1, is expressed on T cells suppresses effector functions when it binds to its ligand, PD-L1. Aberrant PD-L1 expression reported various cancers considered an escape mechanism. Antibodies blocking PD-1/PD-L1 axis induce antitumour responses patients...
Abstract Immunotherapy targeting programmed cell death 1 (PD-1) and PD-ligand (PD-L1) represents promising treatments for human cancers. Our previous studies demonstrated PD-L1 overexpression in some canine cancers, suggested the therapeutic potential of a chimeric anti-PD-L1 monoclonal antibody (c4G12). However, such evidence is scarce, limiting clinical application dogs. In present report, expression was assessed various cancer types, using new mAb, 6C11-3A11, safety efficacy c4G12 were...
The biodegradation of nylon by lignin-degrading fungi was investigated. fungus IZU-154 significantly degraded nylon-66 membrane under ligninolytic conditions. Nuclear magnetic resonance analysis showed that four end groups, CHO, NHCHO, CH3, and CONH2, were formed in the biodegraded membranes, suggesting oxidatively.
A combination of irradiation and oclacitinib, a Janus kinase (JAK) inhibitor used in dogs, could lead to synergistic anticancer effects canine tumors. However, the anti-tumor oclacitinib remain unclear. This study investigated radio-sensitizing effect tumors determined its underlying mechanisms using osteosarcoma (HMPOS), malignant melanoma (CMeC), thyroid adenocarcinoma (CTAC) cell lines. clonogenic assay tumor growth assessment xenograft mouse model (BALB/cAJcl-nu/nu) were performed...
Although immune checkpoint inhibitors (ICIs), such as the anti-programmed death-ligand 1 (PD-L1) antibody, have been developed for treatment of canine malignant melanoma, desirable clinical efficacies not achieved. Recent studies in humans suggested that radiation therapy (RT) combined with ICIs induces robust systemic antitumour immunity patients cancer. This study retrospectively examined therapeutic efficacy combination (hypofractionated RT and anti-PD-L1 antibody [c4G12]) dogs pulmonary...
To compare the survival of dogs with completely resected massive hepatocellular carcinoma (HCC) that in which HCC were incompletely excised.A retrospective cohort study was conducted. Dogs underwent surgical excision between November 2006 and April 2015 included. died perioperative period or lost to follow-up within 2 months after surgery excluded. Data collected from medical records a single pathologist examined all available histology slides confirm diagnosis HCC. Surgical margins defined...
Abstract Immune checkpoint inhibitors (ICIs) such as anti-PD-L1 antibodies are widely used to treat human cancers, and growing evidence suggests that ICIs promising treatments for canine malignancies. However, only some oral malignant melanoma (OMM) cases respond ICIs. To explore biomarkers predictive of survival in dogs with pulmonary metastatic OMM receiving the antibody c4G12 ( n = 27), serum concentrations prostaglandin E2 (PGE 2 ), cytokines, chemokines, growth factors were measured...
Spontaneous tumors are a major cause of death in cats. Treatment human has progressed dramatically the past decade, partly due to success immunotherapies using immune checkpoint inhibitors, such as anti-programmed 1 (PD-1) and anti-PD-ligand (PD-L1) antibodies. However, little is known about PD-1 pathway its association with tumor disease This study investigated applicability anti-PD-1/PD-L1 therapy feline tumors. We first determined complete coding sequence PD-L1 PD-L2 , found that deduced...
Accumulating evidence supporting the cancer stem cell (CSC) hypothesis is based on finding that tumors contain a small population of self-renewing cells generate differentiated progeny and thereby contribute to tumor heterogeneity. CSCs are reported exist in several human cancers, yet only few reports demonstrate existence primary lung dogs. In this study, authors established line derived from canine adenocarcinoma identified side (SP) displayed drug-resistant features. To confirm...
Four isozymes of manganese peroxidase (MnP) were identified in the culture fluid hyperlignolytic fungus IZU-154 under nitrogen starvation conditions. One them was purified and characterized kinetically. The specific activity Kcat/K(m) value MnP from 1.6 times higher than those a typical lignin-degrading fungus, Phanerochaete chrysosporium. Two cDNAs encoding isolated. coding sequence two cDNAs, IZ-MnP1 cDNA IZ-MnP2 cDNA, 1,152 (384 amino acids) 1,155 (385 bp length, respectively. They...
Cancer stem-like cells (CSCs)/cancer-initiating (CICs) are a small subpopulation of cancer that responsible for the initiation, recurrence and metastasis cancer. We previously demonstrated that, using Hoechst 33342 dye-based side population technique, CSCs/CICs in canine lung adenocarcinoma cell line exist. In this study, as known to form spheres anchorage-independent environment vitro, we evaluated stemness spheroid derived from osteosarcoma by expression markers, investigated...
Immune checkpoint inhibitors (ICIs) have been developed for canine tumour treatment, and pilot clinical studies demonstrated their antitumour efficacy in dogs with oral malignant melanoma (OMM). Although ICIs approved various human malignancies, benefits other types remain to be elucidated dogs. Here, we conducted a study of c4G12, chimeric anti-PD-L1 antibody, assess its safety advanced tumours (n = 12) at the Veterinary Teaching Hospital Hokkaido University from 2018 2023. Dogs digit or...
Abstract Background Increased serum cortisol (COR) concentrations may induce glucocorticoid resistance by down‐regulation of receptor (GCR), resulting in decreased chemotherapy efficacy dogs with lymphoma. Hypothesis Investigate the relationship between COR and outcomes Animals Thirty client‐owned lymphoma, concentration measured using samples collected at diagnosis. Methods Retrospective study. Dogs were divided into 2 groups based on concentrations: a normal group (n = 16) <6 μg/dL high...
Metformin has many anti-cancer effects, alone or in combination with radiation. However, the mechanism underlying its radio-sensitized effect is still unclear, especially for cancer stem-like cells (CSCs). Here, of metformin was investigated, and revealed CSCs derived from canine osteosarcoma cell line (HMPOS), a line. Spheroid (SCs) were used as CSCs-rich sphere formation, SCs compared normal adherent culture (ACs). The radio-sensitizing using clonogenic assay tumor growth mice xenograft...
Expression of programmed death ligand 1 (PD-L1) on tumour cells provides an immune evasion mechanism by inducing suppression cytotoxic T cells. Various regulatory mechanisms PD-L1 expression have been described in human tumours, however, little is known canine tumours. To investigate whether inflammatory signalling involved regulation the effects interferon (IFN)-γ and necrosis factor (TNF)-α treatment were examined malignant melanoma cell lines (CMeC LMeC) osteosarcoma line (HMPOS). The...
The transcription factor hypoxia-inducible 1α (HIF-1α) drives metabolic reprogramming in gliomas (GLs) under hypoxic conditions, promoting glycolysis for tumor development. Evofosfamide (EVO) releases a DNA-alkylating agent within regions, indicating that it may serve as hypoxia-targeted therapy. aim of this study was to investigate the glycolytic metabolism and antitumor effects EVO canine GL model. Our clinical data showed overall survival significantly decreased dog patients with higher...
A 10-year-old cat presented for evaluation with a 1-month history of salivation and oral bleeding.A right mandibular mass was palpated computed tomography examination revealed entire bone proliferation.Mandibular biopsy performed, histopathological diagnosis vascular hamartoma.The suddenly died on day 140.
Cancer stem-like cells (CSCs) are self-renewing comprising a small subpopulation in tumours, and generate differentiated progeny through asymmetric division. It has been shown that CSCs resistant to ionizing radiation, this feature could be one of the mechanisms tumour recurrence after radiation therapy. Much attention focused on target CSCs; however, difficult isolating lack knowledge their radiosensitivity have limited kind research veterinary medicine. In present study, sphere-forming...
Cancer stem-like cells (CSCs) cause treatment failure in various tumours; however, establishing CSC-targeted therapies has been hampered by difficulties the identification and isolation of this small sub-population cells. Recent studies have revealed that tumour with low proteasome activity display a CSC phenotype can be utilized to image CSCs canines. This study visualizes reveals CSC-like properties HMPOS (osteosarcoma) MegTCC (transitional cell carcinoma), which are canine lines. The...
A 10-year-old castrated male cross-breed dog was referred for the repair of perineal hernia with bladder retroflexion and a mass lesion in hernial sac. Surgical treatment performed identified as degenerated adipose tissue that suspected to be derived from omentum. The contents were reduced without difficulty, exhibited normal recovery. Two days after surgery, suddenly anorexia azotemia. Exploratory laparotomy performed, which showed dilation both ureters discoloration serosa strangulation...
Allogeneic hematopoietic cell transplantation (HCT) has been an effective treatment for human patients with haematological malignancies (Baron & Storb, 2006; Bair et al., 2020; Copelan 2019). However, the optimal pretransplant conditioning is unclear in canine allogeneic HCT. This pilot study aimed to evaluate safety and efficacy of total lymphoid irradiation (TLI) volumetric modulated arc therapy (VMAT) a nonmyeloablative HCT conditioning. Six healthy dogs were treated 8 or 12 Gy TLI using...