A5016045126- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
- Advanced Memory and Neural Computing
- EEG and Brain-Computer Interfaces
- Functional Brain Connectivity Studies
- Neural Networks and Applications
- Ion channel regulation and function
- Muscle activation and electromyography studies
- Photoreceptor and optogenetics research
- Ion Channels and Receptors
- Essential Oils and Antimicrobial Activity
- Blind Source Separation Techniques
- Visual perception and processing mechanisms
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- Robotics and Automated Systems
- Memory and Neural Mechanisms
- Neural and Behavioral Psychology Studies
- Neurogenesis and neuroplasticity mechanisms
- Algorithms and Data Compression
- Epilepsy research and treatment
Northwestern University
2010-2023
Northwestern University
2022
Institute of Science and Technology Austria
2017-2021
Jülich Aachen Research Alliance
2016
Forschungszentrum Jülich
2016
University of Bonn
2000
Physiological measurements in neuroscience experiments often involve complex stimulus paradigms and multiple data channels. Ephus (http://www.ephus.org) is an open-source software package designed for general-purpose acquisition instrument control. operates as a collection of modular programs, including ephys program standard whole-cell recording with single or electrodes typical electrophysiological experiments, mapper synaptic circuit mapping involving laser scanning photostimulation based...
Biophysical modeling of neuronal networks helps to integrate and interpret rapidly growing disparate experimental datasets at multiple scales. The NetPyNE tool (www.netpyne.org) provides both programmatic graphical interfaces develop data-driven multiscale network models in NEURON. clearly separates model parameters from implementation code. Users provide specifications a high level via standardized declarative language, for example connectivity rules, create millions cell-to-cell...
The motor cortex (M1) is classically considered an agranular area, lacking a distinct layer 4 (L4). Here, we tested the idea that M1, despite cytoarchitecturally visible L4, nevertheless possesses its equivalent in form of excitatory neurons with input–output circuits like those L4 sensory areas. Consistent this idea, found located thin laminar zone at L3/5A border forelimb area mouse M1 have multiple L4-like synaptic connections: input from thalamus, largely unidirectional outputs to L2/3...
Motor cortex is a key brain center involved in motor control rodents and other mammals, but specific intracortical mechanisms at the microcircuit level are largely unknown. Neuronal expression of hyperpolarization-activated current ( I h ) cell class throughout nervous system, neocortex, where pyramidal neurons classified various ways, systematic pattern has not been identified. We tested whether differentially expressed among projection classes mouse cortex. was high corticospinal low...
Understanding cortical function requires studying multiple scales: molecular, cellular, circuit, and behavioral. We develop a multiscale, biophysically detailed model of mouse primary motor cortex (M1) with over 10,000 neurons 30 million synapses. Neuron types, densities, spatial distributions, morphologies, biophysics, connectivity, dendritic synapse locations are constrained by experimental data. The includes long-range inputs from seven thalamic regions noradrenergic inputs. Connectivity...
Corticospinal pyramidal neurons mediate diverse aspects of motor behavior. We measured spike-related electrophysiological properties identified corticospinal in primary cortex slices from young adult mice. Several consistent features were observed the suprathreshold responses to current steps: 1) fired relatively fast action potentials (APs; width at half-maximum 0.65 ± 0.13 ms, mean standard deviation [SD]) compared with neighboring callosally projecting corticostriatal neurons. AP was...
Primary motor (M1) and secondary somatosensory (S2) cortices, although anatomically functionally distinct, share an intriguing cellular component: corticospinal neurons (CSP) in layer 5B. Here, we investigated the long-range circuits of CSPs mouse forelimb-M1 S2. We found that interareal projections (S2 → M1 S2) monosynaptically excited pyramidal across multiple layers, including CSPs. Area-specific differences were observed relative strengths inputs to subsets other cell types, but general...
Summary: Purpose : Hilar mossy cells represent an important excitatory subpopulation of the hippocampal formation. Several studies have identified this cell type as particularly vulnerable to seizure activity in rat models limbic epilepsy. Here we subjected hilar loss hippocampus patients with chronic temporal lobe epilepsy (TLE) a systematic morphological and immunohistochemical analysis. Methods Hippocampal specimens from 30 TLE were included; 21 presented segmental neuronal [Ammon's horns...
Corticospinal neurons (SPI), thick-tufted pyramidal in motor cortex layer 5B that project caudally via the medullary pyramids, display distinct class-specific electrophysiological properties vitro: strong sag with hyperpolarization, lack of adaptation, and a nearly linear frequency-current ( F– I) relationship. We used our data to produce pair large archives SPI neuron computer models two model classes: 1) detailed full reconstruction; 2) simplified six compartments. PRAXIS an evolutionary...
Auditory cortex (AC) layer 5B (L5B) contains both corticocollicular neurons, a type of pyramidal-tract neuron projecting to the inferior colliculus, and corticocallosal intratelencephalic contralateral AC. Although it is known that these neuronal types have distinct roles in auditory processing different response properties sound, synaptic intrinsic mechanisms shaping their input-output functions remain less understood. Here, we recorded brain slices mouse AC from retrogradely labeled...
A set of methods is described for channelrhodopsin-2 (ChR2)-based synaptic circuit analysis that combines photostimulation virally transfected presynaptic neurons’ axons with whole-cell electrophysiological recordings from retrogradely labeled postsynaptic neurons. The approach exploits the preserved photoexcitability ChR2-expressing in brain slices and can be used to assess either local or long-range functional connections. Stereotaxic injections are both express ChR2 selectively interest...
This article was motivated by the conference entitled 'Perception & Action – An Interdisciplinary Approach to Cognitive Systems Theory', which took place September 14-16, 2010 at Santa Fe Institute, NM, U.S.A. The goal of bring together an interdisciplinary group neuroscientists, roboticists and theorists discuss extent implications action-perception integration in brain. motivation for realization that it is a widespread approach biological, theoretical computational neuroscience...
The deceptively simple laminar structure of neocortex belies the complexity intra- and interlaminar connectivity. We developed a computational model based primarily on unified set brain activity mapping studies mouse M1. simulation consisted 775 spiking neurons 10 cell types with detailed population-to-population Static analysis connectivity graph-theoretic tools revealed that corticostriatal population showed strong centrality, suggesting would provide network hub. Subsequent dynamical...
Abstract Understanding cortical function requires studying multiple scales: molecular, cellular, circuit and behavior. We developed a biophysically detailed multiscale model of mouse primary motor cortex (M1) with over 10,000 neurons 30 million synapses. Neuron types, densities, spatial distributions, morphologies, biophysics, connectivity dendritic synapse locations were tightly constrained by experimental data. The includes long-range inputs from 7 thalamic regions, as well noradrenergic...
Abstract We developed a biophysically detailed multiscale model of mouse primary motor cortex (M1) with over 10,000 neurons and 35 million synapses. focused on intratelencephalic (IT) pyramidal-tract (PT) layer 5 (L5), which were modeled at high multicompartment resolution. Wiring densities based prior measures from slice, depended cell class cortical depth sublaminar Prominent phase-amplitude-coupled delta gamma activity emerged the network. Spectral Granger causality analysis revealed...
Blocking the TRPV1 receptor is an interesting approach for treatment of sensitive skin. Here we investigated potential grifolin derivatives from Albatrellus ovinus to act as blockers and their serve cosmetic active ingredients.Binding characteristics were determined in competitive functional vitro assays achieve IC50 values. The was activated vivo with capsaicin noxious heat investigate skin reddening, microcirculation, sensations pain thresholds.Grifolin extracted proved inhibit vivo....
ABSTRACT Pattern separation is a fundamental brain computation that converts small differences in synaptic input patterns into large action potential (AP) output patterns. plays key role the dentate gyrus, enabling efficient storage and recall of memories downstream hippocampal CA3 networks. Several mechanisms for pattern have been proposed, including expansion coding space, sparsification neuronal activity, simple thresholding mechanisms. Alternatively, winner-takes-all mechanism, which...
We have developed a hybrid model of motor control for brain-machine interface that is based on large-scale primary cortex (M1) several mammalian studies (Figure (Figure1).1). The M1 consisted 10,000 spiking Izhikevich neurons four types: regular-firing and bursting pyramidal neurons, fast-spiking low-threshold-spiking interneurons. Within the population, cell proportions, locations, connectivity delays were drawn primarily from mouse experimental data. Properties body cortical depth...