A5004829263- Innovative Microfluidic and Catalytic Techniques Innovation
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Microfluidic and Capillary Electrophoresis Applications
- Nanocluster Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Nanowire Synthesis and Applications
- Polymer Surface Interaction Studies
- Nanopore and Nanochannel Transport Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Electrohydrodynamics and Fluid Dynamics
- Nanofabrication and Lithography Techniques
- Membrane Separation Technologies
- Medical Imaging and Pathology Studies
- Force Microscopy Techniques and Applications
University of Copenhagen
2018-2023
University of British Columbia
2018-2023
Precision Nanosystems (Canada)
2023
H & N Instruments (United States)
2017
Denison University
2017
Engineered T cells expressing chimeric antigen receptors (CARs) have been proven as efficacious therapies against selected hematological malignancies. However, the approved CAR cell therapeutics strictly rely on viral transduction, a time- and cost-intensive procedure with possible safety issues. Therefore, direct transfer of in vitro transcribed CAR-mRNA into is pursued promising strategy for engineering. Electroporation (EP) currently used mRNA delivery method generation clinical trials...
The structural shift of a DNA hairpin-dimer is as important the sequence in determining fluorescent properties DNA-stabilized silver nanoclusters (DNA/AgNCs).
Fluorescent, DNA-stabilized silver nanoclusters (DNA-AgNCs) are applied in a range of applications within nanoscience and nanotechnology. However, their diverse optical properties, mechanism formation, aspects composition remain unexplored, making the rational design nanocluster probes challenging. Herein, synthetic procedure is described for obtaining high yield emissive DNA-AgNCs with C-loop hairpin DNA sequence, subsequent purification by size-exclusion chromatography (SEC). Through...
Polymeric microfluidic chips offer a number of benefits compared to their glass equivalents, including lower material costs and ease flexibility fabrication. However, the main drawback polymeric materials is often limited resistance (organic) solvents. Previously, thiol-ene were shown be more solvent resistant than most other commonly used polymers; however, they still fall short in "harsh" chemical environments, such as when chlorinated solvents are present. Here, we show that simple yet...
Nanomedicines based on nanoparticles rely both the potency of drug as well efficiency delivery system, for which particle size plays a crucial role. For intracellular small interference RNA (siRNA), lipid-polymer nanoparticle (LPN) hybrid systems constitute safe and highly effective class systems. In present study, we employ microfluidics method manufacturing spherical siRNA-loaded LPNs pulmonary with distinct distributions average diameters approximately 70, 110, 220 nm. We designed an...
Abstract Fluorescent, DNA‐stabilized silver nanoclusters (DNA‐AgNCs) are applied in a range of applications within nanoscience and nanotechnology. However, their diverse optical properties, mechanism formation, aspects composition remain unexplored, making the rational design nanocluster probes challenging. Herein, synthetic procedure is described for obtaining high yield emissive DNA‐AgNCs with C‐loop hairpin DNA sequence, subsequent purification by size‐exclusion chromatography (SEC)....
Albumin is widely used in pharmaceutical applications to alter the pharmacokinetic profile, improve efficacy, or decrease toxicity of active compounds. Various drug delivery systems using albumin have been reported, including microparticles. Macroaggregated (MAA) one more common forms microparticles, which predominately for lung perfusion imaging when labeled with radionuclide technetium-99m (99mTc). These microparticles are formed by heat-denaturing a bulk solution, making it very...
A method is described for the sensitive measurement of adsorbed proteins using femtoliter microwells. Quantitative protein demonstrated at surface densities from 10 fg/cm2 to 3 pg/cm2. Determination efficacy barrier coatings also Adsorption low measured, indicating highest affinity sites on and therefore initial stages adsorption. The microwell shown be useful in detecting differences between effective protective coatings.
Watch the video or read poster to learn more about: Pros and cons of current gene delivery mechanisms need for improved solutionsBenefits LNPs effective nucleic acids therapy, editing, mRNA vaccine applicationsHow process innovation is enabling rapid, scalable, reproducible LNP productionCase study: optimizing mRNA-LNP formulations CAR T cell editingReka holds a PhD in Pharmaceutical Sciences from University British Columbia with high impact publications at interface microfluidics drug...