A5003105711- Ultrasound Imaging and Elastography
- Ultrasound and Hyperthermia Applications
- Congenital heart defects research
- Photoacoustic and Ultrasonic Imaging
- Advanced MRI Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Ultrasonics and Acoustic Wave Propagation
- Cardiomyopathy and Myosin Studies
- Cell Image Analysis Techniques
- Gene expression and cancer classification
- Pluripotent Stem Cells Research
- Molecular Biology Techniques and Applications
- Fetal and Pediatric Neurological Disorders
- Cardiac electrophysiology and arrhythmias
- Advanced Fluorescence Microscopy Techniques
- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Congenital Heart Disease Studies
- Angiogenesis and VEGF in Cancer
- Cardiovascular Effects of Exercise
- Medical Image Segmentation Techniques
- Cardiovascular Function and Risk Factors
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- AI in cancer detection
New York University
2016-2025
Advanced Imaging Research (United States)
2023-2024
Riverside Research Institute
2008-2019
Children's Hospital of Philadelphia
1998-2004
Vanderbilt University
2004
University of Rochester
2003
Center for Human Genetics
2003
University Medical Center
1998-2002
La Roche College
1998
Universidad Pontificia Bolivariana
1993
To elucidate the role of cardiac myosin-binding protein-C (MyBP-C) in myocardial structure and function, we have produced mice expressing altered forms this sarcomere protein. The engineered mutations encode truncated MyBP-C which myosin heavy chain-binding titin-binding domain has been replaced with novel amino acid residues. Analogous heterozygous defects humans cause hypertrophic cardiomyopathy. Mice that are homozygous for mutated alleles express less than 10% protein M-bands otherwise...
This paper investigates the feasibility of fabricating a five-ring, focused annular array transducer operating at 40 MHz. The active piezoelectric material was 9-microm thick polyvinylidene fluoride (PVDF) film. One side PVDF metallized with gold and forms ground plane transducer. pattern electrical traces to each annulus were formed on copper-clad polyimide bonded thin layer epoxy, pressed into spherically curved shape, then back filled epoxy. A five-ring equal area elements 100-microm...
When cardiac function and blood flow are first established fundamental questions in mammalian embryogenesis. The earliest erythroblasts arise yolk sac islands subsequently enter the embryo proper to initiate circulation. Embryos staged 0 30 somites (S) were examined utero with 40- 50-MHz ultrasound biomicroscopy (UBM)-Doppler, determine onset of embryonic heartbeat characterize basic physiology very early mouse A was detected at 5 S, vascular 7 S. Heart rate, peak arterial velocity,...
Background —The increasing number of transgenic and targeted mutant mice with embryonic cardiac defects has resulted in the need for noninvasive techniques to examine structure function early mouse embryos. We report first use a novel 40-MHz ultrasound imaging system study development utero. Methods Results —Transabdominal scans embryos staged between 8.5 13.5 days gestation (E8.5 E13.5) were obtained anesthetized mice. Atrial ventricular contractions could be discerned from E9.5, changes...
Triple-negative breast cancer (TNBC) lacks expressed protein targets, making therapy development challenging. Hydrogels offer a promising new route in this regard by improving the chemotherapeutic efficacy through increased solubility and sustained release. Moreover, subcutaneous hydrogel administration reduces patient burden requiring less shorter treatment times. We recently established design principles for supramolecular assembly of single-domain coiled-coils into hydrogels. Using...
Heterozygous mice bearing an Arg403Gln missense mutation in the α cardiac myosin heavy chain gene (α-MHC403/+) exhibit histopathologic features of human familial hypertrophic cardiomyopathy. Surprisingly, homozygous α-MHC403/403 die by postnatal day 8. Here we report that neonatal lethality is caused a fulminant dilated cardiomyopathy characterized myocyte dysfunction and loss. Heart tissues from wild-type demonstrate equivalent switching MHC isoforms; isoforms each increase 30% at birth to...
The cortex is thought to be the primary site of sensory plasticity, particularly during development. Here, we report that large-scale reorganization mouse auditory midbrain tonotopic map induced by a specific sound-rearing environment consisting paired low- (16 kHz) and high-frequency (40 tones. To determine potential for plasticity in midbrain, used manganese-enhanced MRI analyze maps control mice normal development reared two-tone + 40 environment. We found emerged third postnatal week...
Embryonic development in many species, including case reports humans, can be temporarily halted before implantation during a process called diapause. Facultative diapause occurs under conditions of maternal metabolic stress such as nursing. While molecular mechanisms have been studied, natural inducing factor has yet to identified. Here, we show that oxytocin induces embryonic mice. We gestational delays were triggered nursing or optogenetic stimulation neurons simulating patterns. Mouse...
Gene targeting in the mouse has become a standard approach, yielding important new insights into genetic factors underlying cardiovascular development and disease. However, we still have very limited understanding of how mutations affect developing function, few studies been performed to measure altered physiological parameters mutant embryos. Indeed, although utero lethality due embryonic heart failure is one most common results gene experiments mouse, mechanisms responsible for demise...
Congenital heart disease results from genetic defects that are manifested at early stages of embryogenesis. The mouse is the preferred animal model for studies mammalian embryonic development and an increasing number human models. A genes identified in critical normal cardiovascular development, but understanding underlying mechanisms regulating still incomplete, part because lack methods to measure hemodynamics live embryos. We describe a 40-MHz ultrasound scanner, which allows image-guided...
An experimental system to take advantage of the imaging capabilities a 5-ring polyvinylidene fluoride (PVDF)-based annular array is presented. The has 6-mm total aperture and 12-mm geometric focus. designed pulse single element then digitize received data all channels simultaneously. All transmit/receive pairs are digitized post-processed with synthetic-focusing technique achieve an enhanced depth field (DOF). performance experimentally tested wire phantom consisting 25-microm diameter wires...
Characterizing embryonic circulatory physiology requires accurate cardiac output and flow data. Despite recent applications of high-frequency ultrasound Doppler to the study circulation, current analysis volumetric is relatively crude. To improve derivation flow, we sought a preliminary model spatial velocity profile in mouse dorsal aorta using biomicroscopy (UBM)-Doppler Embryonic hematocrit 0.05-0.10 so rheologic properties must be insignificant. Low Reynolds numbers (<500) Womersley...
The spatial resolution of high-frequency ultrasound (HFU, >20 MHz) imaging systems is usually determined using wires perpendicular to the beam. Recently, two tissue-mimicking phantoms (TMPs) were developed estimate three-dimensional (3-D) resolution. Each TMP consists nine 1-cm-wide slabs material containing randomly distributed anechoic spheres. All spheres in one slab have same dimensions, and their diameter increased from 0.1 mm first 1.09 last. scattering background for set was...
The formation and maintenance of a functional vasculature is essential for normal embryonic development, genetic changes that affect the underlie pathogenesis in many human diseases. In vivo imaging mouse models required to understand full complexity mammalian vascular formation, which dynamic 3-dimensional process. Optical microscopy genetically expressed fluorescent reporter proteins offers high resolution but limited depth penetration vivo. Conversely, there are plethora molecular probes...
We propose a fully automatic segmentation method called nested graph cut to segment images (2D or 3D) that contain multiple objects with structure. Compared other graph-cut-based methods developed for regions, our can work well without requiring manual selection of initial seeds, even if different have similar intensity distributions and some object boundaries are missing. Promising results were obtained separating the brain ventricles, head, uterus region in mouse-embryo head using...
Mouse models have increased our understanding of the pathogenesis medulloblastoma (MB), most common malignant pediatric brain tumor that often forms in cerebellum.A major goal ongoing research is to better understand early stages tumorigenesis and establish genetic environmental changes underlie MB initiation growth.However, studies progression mouse are difficult due heterogeneity onset times growth patterns lack clinical symptoms at stages.Magnetic resonance imaging (MRI) critical for...
The spatial resolution of high-frequency ultrasound (HFU, >20 MHz) imaging systems is usually determined using wires perpendicular to the beam. Recently, two tissue-mimicking phantoms (TMPs) were developed estimate three-dimensional (3-D) resolution. Each TMP consists nine 1-cm-wide slabs material containing randomly distributed anechoic spheres. All spheres in one slab have same dimensions, and their diameter increased from 0.1 mm first 1.09 last. scattering background for set was...
Segmentation and mutant classification of high-frequency ultrasound (HFU) mouse embryo brain ventricle (BV) body images can provide valuable information for developmental biologists. However, manual segmentation identification BV requires substantial time expertise. This article proposes an accurate, efficient explainable deep learning pipeline automatic the body. For segmentation, a two-stage framework is implemented. The first stage produces low-resolution map, which then used to crop...
This paper presents an adaptive synthetic-focusing scheme that, when applied to photoacoustic (PA) data acquired using annular array, improves focusing across a greater imaging depth and enhances spatial resolution. The system was based on 40-MHz, 5-element, annular-array transducer with focal length of 12 mm 800-µm diameter hole through its central element facilitate coaxial delivery 532-nm laser. raster-scanned 3D acquisition co-registered ultrasound PA image data. Three schemes were...