A5069537554- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Bat Biology and Ecology Studies
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Neonatal and fetal brain pathology
- Mitochondrial Function and Pathology
- Axon Guidance and Neuronal Signaling
- Adipose Tissue and Metabolism
- Erythrocyte Function and Pathophysiology
- Circadian rhythm and melatonin
- Memory and Neural Mechanisms
- Amyotrophic Lateral Sclerosis Research
- Neuroscience of respiration and sleep
- Tissue Engineering and Regenerative Medicine
- Dietary Effects on Health
- Mesenchymal stem cell research
- Eicosanoids and Hypertension Pharmacology
- Neural dynamics and brain function
- Sleep and Wakefulness Research
- Pluripotent Stem Cells Research
- Kruppel-like factors research
- Neuroinflammation and Neurodegeneration Mechanisms
- ATP Synthase and ATPases Research
- Stress Responses and Cortisol
Universidad Politécnica de Madrid
2013-2025
Universidad San Pablo CEU
2015-2025
Universidad San Pablo
2023
Instituto Cajal
2012-2019
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2016-2018
Hospital Clínico San Carlos
2016-2018
Universidad Complutense de Madrid
2016-2018
Unidad Funcional de Investigación de Enfermedades Crónicas
2014
Instituto de Salud Carlos III
2011-2014
Biomedical Research Networking Center on Neurodegenerative Diseases
2012
Almost a 20% of the residues tau protein are phosphorylatable amino acids: serine, threonine, and tyrosine. In this paper we comment on consequences for being phosphoprotein. We will focus serine/threonine phosphorylation. It be discussed that, depending modified residue in molecule, phosphorylation could protective, processes like hibernation, or toxic development those diseases known as tauopathies, which characterized by an hyperphosphorylation aggregation tau.
Abstract Pyramidal neurons are the most common cell type and considered main output neuron in mammalian forebrain structures. In terms of function, differences structure dendrites these appear to be crucial determining how integrate information. To further shed light on human pyramidal we investigated geometry cells mouse CA1 region—one evolutionary conserved archicortical regions, which is critically involved formation, consolidation, retrieval memory. We aimed assess what extent...
Despite extensive studies regarding tau phosphorylation progression in both human Alzheimer's disease cases and animal models, the molecular structural changes responsible for neurofibrillary tangle development are still not well understood.Here, by using antibodies AT100 (recognizes protein phosphorylated at Thr212 Ser214 proline-rich region) pS396 serine residue 396 C-terminal region), we examined phospho-tau immunostaining neurons from hippocampal CA1 region of 21 with pathology ranging...
The output of cortical pyramidal cells reflects the balance between excitatory inputs and subcortical origin, inhibitory from distinct populations GABAergic interneurons, each which selectively innervate differen
Syrian hamsters undergo a reversible hyperphosphorylation of protein τ during hibernation, providing unique natural model that may unveil the physiological mechanisms behind this critical process involved in development Alzheimer's disease and other tauopathies. The hibernation cycle these animals fluctuates between pair stages: 3–4 days torpor bouts interspersed with periods euthermia called arousals last several hours. In study, we investigated for first time metabolic changes brain tissue...
Tau is a cytoskeletal protein present mainly in the neurons of vertebrates. By comparing sequence tau molecule among different vertebrates, it was found that variability N‐terminal higher than C‐terminal region. The region involved binding to cellular membranes, whereas contains microtubule‐binding sites. We have compared Syrian hamster with sequences other hibernating and nonhibernating rodents investigated how differences could affect phosphorylation level cell membranes. also describe...
Abstract The axon initial segment (AIS) is a region of the neuron that critical for action potential generation as well regulation neural activity. This specialized structure—characterized by expression different types ion channels adhesion, scaffolding and cytoskeleton proteins—is subjected to morpho-functional plastic changes in length position upon variations activity or pathological conditions. In present study, using immunocytochemistry with AT8 antibody (phospho-tau S202/T205) 3D...
Hibernating animals have been used as models to study several aspects of the plastic changes that occur in metabolism and physiology neurons. These are also interest Alzheimer's disease because microtubule-associated protein tau is hyperphosphorylated during hibernation state known torpor, similar pretangle stage disease. undergo torpor periods with drops body temperature metabolic rate, a virtual cessation neural activity. processes accompanied by morphological neurochemical neurons, which...
Abstract Endogenous neurogenesis in stroke is insufficient to replace the lost brain tissue, largely due lack of a proper biological structure let new cells dwell damaged area. We hypothesized that scaffolds made hyaluronic acid (HA) biomaterials (BM) could provide suitable environment home not only neurons, but also vessels, glia and neurofilaments. Further, addition exogenous cells, such as adipose stem (ASC) increase this effect. Athymic mice were randomly assigned one four group: alone,...
La exposición de líquido cefalorraquídeo (LCR) pacientes con esclerosis lateral amiotrófica (ELA) induce efectos citotóxicos en cultivos celulares neuronas motoras in vitro. Se seleccionó LCR 32 ELA que previamente habían demostrado citotóxicos. implantaron minibombas osmóticas intracerebroventriculares (ICV) 28 ratas macho adultas y se dividieron 3 grupos: 9 no-ELA, 15 ELA-LCR citotóxico 4 una solución salina fisiológica. El administró por vía ICV forma continua durante periodos 20 o 43...
Sprouty and Spred proteins have been widely implicated in the negative regulation of fibroblast growth factor receptor-extracellular regulated kinase (ERK) pathway. In considering functional role these proteins, we explored their effects on ERK activation induced by cyclopentenone prostanoids, which bind to activate Ras proteins. We therefore found that ectopic overexpression HeLa cells human Sprouty2, or Spred1 2, inhibits ERK1/2 Elk-1 triggered prostanoids PGA1 15d-PGJ2. Furthermore, HT do...
Cerebrospinal fluid (CSF) from amyotrophic lateral sclerosis (ALS) patients induces cytotoxic effects in vitro cultured motor neurons. We selected CSF with previously reported 32 ALS patients. Twenty-eight adult male rats were intracerebroventricularly implanted osmotic mini-pumps and divided into 3 groups: 9 injected non-ALS patients, 15 ALS-CSF, 4 a physiological saline solution. was continuously infused for periods of 20 or 43 days after implantation. conducted clinical assessments...
The Shoc2 protein has been implicated in the positive regulation of Ras-ERK pathway by increasing functional binding interaction between Ras and Raf, leading to increased ERK activity. Here we found that overexpression induced sustained phosphorylation, notably case EGF stimulation, knockdown inhibited activation. We demonstrate ectopic human PC12 cells significantly promotes neurite extension presence EGF, a stimulus induces proliferation rather than differentiation these cells. Finally,...
Abstract The dendritic spines of pyramidal cells are the main postsynaptic target excitatory glutamatergic synapses. Morphological alterations have been described in hippocampal during hibernation—a state inactivity and metabolic depression that occurs via a transient neuronal tau hyperphosphorylation. Here, we used hibernating Syrian hamster to investigate effect hyperphosphorylated regarding neocortical structure. In particular, examined layer Va neurons. Our results indicate hibernation...
Resumen La enfermedad de Alzheimer (EA) es la forma más común demencia y tiene una elevada morbilidad mortalidad. EA se caracteriza principalmente por presencia dos estructuras aberrantes en el cerebro los pacientes, placas seniles formadas péptido-β-amiloide (Aβ) ovillos neurofibrilares cuyo principal componente proteína tau fosforilada. Aunque actualmente no conoce bien etiopatogenia, cada vez son estudios que demuestran un efecto causal del microbioma intestinal sobre las funciones...
Hibernating mammals undergo torpor periods characterized by a general decrease in body temperature, metabolic rate, and brain activity accompanied complex adaptive changes that appear to protect the from extreme conditions of hypoxia low temperatures. These processes are morphological neurochemical including those cortical neurons such as fragmentation reduction Golgi apparatus (GA), which both reverse few hours after arousal state. In present study, we - immunofluorescence confocal...
Hibernation in small mammals is a highly regulated process with periods of torpor involving drops body temperature and metabolic rate, as well general decrease neural activity, all which proceed alongside complex brain adaptive changes that appear to protect the from extreme hypoxia low temperatures. All these are rapidly reversed, no apparent damage occurring, during short arousal, interspersed torpor—characterized by transitory partial rewarming including sleep activation, feeding some...