A5069343282- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Bat Biology and Ecology Studies
- Genetics and Neurodevelopmental Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Gut microbiota and health
- Cerebrovascular and genetic disorders
- Cerebrovascular and Carotid Artery Diseases
- Tryptophan and brain disorders
- Genetics, Aging, and Longevity in Model Organisms
- Folate and B Vitamins Research
- Ion channel regulation and function
- Regulation of Appetite and Obesity
- Biochemical Analysis and Sensing Techniques
- Neurological Disease Mechanisms and Treatments
- Erythrocyte Function and Pathophysiology
- Pluripotent Stem Cells Research
- Neonatal and fetal brain pathology
- Photoreceptor and optogenetics research
- Prion Diseases and Protein Misfolding
- Neuroscience of respiration and sleep
Centro de Biología Molecular Severo Ochoa
2022-2025
Universidad Autónoma de Madrid
2022-2025
Universidad Politécnica de Madrid
2015-2022
Instituto Cajal
2013-2022
University of Edinburgh
2019
UK Dementia Research Institute
2019
MaineGeneral Medical Center
2016-2017
Massachusetts General Hospital
2017
Universidad Complutense de Madrid
2015
Objective Recent studies have shown that positron emission tomography (PET) tracer AV-1451 exhibits high binding affinity for paired helical filament (PHF)-tau pathology in Alzheimer's brains. However, the ability of this ligand to bind tau lesions other tauopathies remains controversial. Our goal was examine correlation vivo and postmortem patterns three autopsy-confirmed non-Alzheimer tauopathy cases. Methods We quantified retention [F-18]-AV-1451 performed autoradiography, [H-3]-AV-1451...
A key knowledge gap blocking development of effective therapeutics for Alzheimer's disease (AD) is the lack understanding how amyloid beta (Aβ) peptide and pathological forms tau protein cooperate in causing phenotypes. Within a mouse tau-deficient background, we probed molecular, cellular, behavioral disruption triggered by influence wild-type human on Aβ-induced pathology. We find that Aβ work cooperatively to cause hyperactivity phenotype downregulation transcription genes involved...
Abstract In recent years, there has been success in partially reprogramming peripheral organ cells using cyclic Yamanaka transcription factor (YF) expression, resulting the reversal of age-related pathologies. case brain, effects partial are scarcely known, and only some its have observed through widespread expression YF. This study is first to exclusively reprogram a specific subpopulation neurons cerebral cortex aged mice. The vivo model demonstrate that YF postmitotic does not...
Ligands targeting folate receptor α (FRα), a protein predominantly expressed in neural cells, have the potential to reprogram (rejuvenate) brain cells and enhance cognitive function aged mice. In this study, we present family of FRα-binding peptides identified through AlphaFold modeling. These induce structural change upon binding, which facilitates its internalization transport cell nucleus. Once nucleus, FRα functions as transcription factor, promoting expression genes associated with...
Neuronal aging may be, in part, related to a change DNA methylation. Thus, methyl donors, like folate and methionine, play role cognitive changes associated neuronal aging. To test the of these metabolites, we performed stereotaxic microinjection molecules into dentate gyrus (DG) aged mice (an average age 21 month). Folate, but not S-Adenosyl-Methionine (SAM), enhances cognition mice. In presence folate, observed partial rejuvenation DG cells, characterized by expression juvenile genes or...
The Golgi apparatus (GA) is a highly dynamic organelle, which mainly involved in the post-translational processing and targeting of cellular proteins undergoes significant morphological changes response to different physiological pathological conditions. In present study, we have analyzed possible alterations GA neurons from temporal neocortex hippocampus Alzheimer's disease (AD) patients, using double immunofluorescence techniques, confocal microscopy 3D quantification techniques. We found...
Abstract The axon initial segment (AIS) is a region of the neuron that critical for action potential generation as well regulation neural activity. This specialized structure—characterized by expression different types ion channels adhesion, scaffolding and cytoskeleton proteins—is subjected to morpho-functional plastic changes in length position upon variations activity or pathological conditions. In present study, using immunocytochemistry with AT8 antibody (phospho-tau S202/T205) 3D...
Hibernating animals have been used as models to study several aspects of the plastic changes that occur in metabolism and physiology neurons. These are also interest Alzheimer's disease because microtubule-associated protein tau is hyperphosphorylated during hibernation state known torpor, similar pretangle stage disease. undergo torpor periods with drops body temperature metabolic rate, a virtual cessation neural activity. processes accompanied by morphological neurochemical neurons, which...
The Golgi apparatus (GA) is a highly dynamic organelle involved in the processing and sorting of cellular proteins. In Alzheimer's disease (AD), it has been shown to decrease size become fragmented neocortical hippocampal neuronal subpopulations. This fragmentation GA AD related accumulation hyperphosphorylated tau. However, involvement other pathological factors associated with course disease, such as extracellular amyloid-β (Aβ) aggregates, cannot be ruled out, since both pathologies are...
The present study aimed to better understand the role of neonatal leptin surge, which peaks on postnatal day (PND)9-10, development hippocampal formation. Accordingly, male and female rats were administered with a pegylated antagonist PND9 expression neurones, glial cells diverse markers synaptic plasticity was then analysed by immunohistochemistry in Antagonism actions at this specific stage altered number fibrillary acidic protein positive cells, also affected type 1 cannabinoid receptors,...
Abstract The vast majority of cortical synapses are found in the neuropil which is implicated multiple and diverse functions underlying brain computation. Unraveling organizing principles requires an intricate characterization synaptic connections established by excitatory inhibitory axon terminals, intrinsic extrinsic origin from ascending projections that govern function microcircuits through release neuromodulators either point-to-point chemical or diffuse volume transmission (VT). Even...
Abstract The Izpisua‐Belmonte group identified a cocktail of metabolites that promote partial reprogramming in cultured muscle cells. We tested the effect brain injection these dentate gyrus aged wild‐type mice. is region essential for memory function and extremely vulnerable to aging. A single containing four compounds (putrescine, glycine, methionine threonine) partially reversed aging phenotypes epigenetic alterations age‐associated genes. Our analysis revealed three levels: chromatin...
Abstract In this work, we have studied the effect of small compounds in partial rejuvenation dentate gyrus cells by measuring improvement cognitive functions elderly mice. Aging has been related to a change DNA methylation and some one-carbon metabolites linked that process, like vitamin B12, folate or methionine involved performance during aging. However, their role process possible mechanisms behind its effects are still unclear. Through direct infusion these molecules tested on cognition...