A5031469247- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Inflammasome and immune disorders
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Drug Transport and Resistance Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Heme Oxygenase-1 and Carbon Monoxide
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Cancer therapeutics and mechanisms
- Lipid Membrane Structure and Behavior
- DNA and Nucleic Acid Chemistry
- Phagocytosis and Immune Regulation
- Extracellular vesicles in disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacteriophages and microbial interactions
- Plasmonic and Surface Plasmon Research
- Trace Elements in Health
- Clostridium difficile and Clostridium perfringens research
- Chemical Synthesis and Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Immune Response and Inflammation
Texas A&M University
2022-2025
Mitchell Institute
2025
University of Kentucky
2014-2023
Vilnius Gediminas Technical University
2021
Yancheng Institute of Technology
2021
Beihang University
2019
Wayne State University
2015
Princeton University
2003-2011
Brookhaven National Laboratory
2004-2006
Rutgers, The State University of New Jersey
2003
Background: Previous studies demonstrated that coplanar polychlorinated biphenyls (PCBs) promote proinflammatory gene expression in adipocytes. PCBs are highly lipophilic and accumulate adipose tissue, a site of insulin resistance persons with type 2 diabetes.Objectives: We investigated the vitro vivo effects on tumor necrosis factor α (TNF-α) glucose homeostasis lean obese mice.Methods: quantified tolerance, as well TNF-α levels, liver, muscle, tissue male C57BL/6 mice administered vehicle,...
Crosstalk between coagulation and innate immunity contributes to the progression of many diseases, including infection cardiovascular disease. Venous thromboembolism (VTE), pulmonary embolism deep vein thrombosis (DVT), is among most common causes death. Here, we show that inflammasome activation subsequent pyroptosis play an important role in development venous thrombosis. Using a flow restriction–induced mouse model inferior vena cava (IVC), deficiency caspase-1, but not caspase-11,...
The cation diffusion facilitators (CDF) are a ubiquitous family of metal transporters that play important roles in homeostasis wide range divalent cations. Molecular identities substrate-binding sites and their selectivity the CDF thus far unknown. By using isothermal titration calorimetry stopped-flow spectrofluorometry, we directly examined binding to highly conserved aspartate Escherichia coli transporter YiiP (FieF). A D157A mutation abolished Cd2+-binding site impaired corresponding...
Combinatorial libraries of de novo amino acid sequences can provide a rich source diversity for the discovery novel proteins. Randomly generated sequences, however, rarely fold into well ordered protein-like structures. To enhance quality library, must be focused those regions sequence space most likely to yield folded We have constructed by designing binary pattern polar and nonpolar acids favor structures that contain abundant secondary structure, while simultaneously burying hydrophobic...
Abstract Binary patterning of polar and nonpolar amino acids has been used as the key design feature for constructing large combinatorial libraries de novo proteins. Each position in a binary patterned sequence is designed explicitly to be either or nonpolar; however, precise identities these are varied extensively. The underpinnings “binary code” strategy preclude explicit particular side chains at specified positions. Therefore, packing interactions cannot priori. To assess whether code...
YiiP is a representative member of the cation diffusion facilitator (CDF) family, class ubiquitous metal transporters that play an essential role in homeostasis. Recently, pair Zn2+/Cd2+-selective binding sites has been localized to two highly conserved aspartyl residues (Asp157), each 2-fold-symmetry-related transmembrane segment 5 (TM5) homodimer. Here we report functional and structural interactions between Asp157 yet another Asp49 TM2. Calorimetric analysis indicated contribute common...
Pyroptosis is a form of proinflammatory cell death characterized by inflammasome activation, pore formation, and the release pro-inflammatory cytokines such as interleukin-1β (IL-1β) IL-18 upon rupture. Nuclear factor-κB (NF-κB), prototypical transcription factor, plays critical role in immune system regulation. Recent research highlights multifaceted roles NF-κB signaling pyroptosis. Various immunologically relevant ligands their receptors can activate pathway to promote pyroptosis, with...
Introduction Klebsiella pneumoniae is a Gram-negative bacterium and the third most commonly isolated microorganism in blood cultures from septic patients. Despite extensive research, mechanisms underlying K. -induced sepsis its pathogenesis remain unclear. Acute respiratory failure leading cause of mortality systemic infections, highlighting need to better understand host immune response bacterial clearance mechanisms. Method To investigate impact infection on organ function response, we...
Systemic blood coagulation accompanies inflammation during severe infection like sepsis and COVID. We’ve previously established a link between pyroptosis, vital defense mechanism against infection, coagulopathy. During the formation of gasdermin-D (GSDMD) pores on plasma membrane leads to release tissue factor (TF)-positive microvesicles (MVs) that are procoagulant. Mice lacking GSDMD fewer TF MVs. However, specific mechanisms leading from activation MV remain unclear. Plasma rupture (PMR)...
Systemic blood coagulation accompanies inflammation during severe infections like sepsis and COVID. We previously established a link between coagulopathy pyroptosis, vital defense mechanism against infection. During the formation of gasdermin-D (GSDMD) pores on plasma membrane leads to release tissue factor (TF)-positive microvesicles (MVs) that are procoagulant. Mice lacking GSDMD fewer these procoagulant MVs. However, specific mechanisms coupling activation MV remain unclear. Plasma...
Highlights•Activation of the NAIP/NLRC4 inflammasome triggers acute lung injury in mice•NAIP/NLRC4 components are expressed macrophages and pulmonary fibroblasts•Acute caused by activation is dependent on pyroptosis•Pyroptosis both fibroblasts contributes to injurySummaryThe plays a pivotal role defense against bacterial infections, with its vivo physiological function primarily recognized as driving inflammation immune cells. Acute (ALI) leading cause mortality sepsis. In this study, we...
Combinatorial libraries of de novo amino acid sequences can provide a rich source diversity for the discovery novel proteins with interesting and important activities. However, since arbitrary rarely fold into well ordered protein‐like structures, randomly generated will yield functional only very rarely. To enhance likelihood finding proteins, we use binary patterning polar non‐polar acids to design focused that are predisposed structures. Proteins isolated from patterned have been shown...
Dual-mode surface-plasmon resonance (SPR) sensors use both long- and short- range surface plasmon waves to differentiate binding interactions from interfering bulk effects. We have optimized the design of these for minimum limit detection (LOD) using a Cramer-Rao lower bound spectral shift estimation. Despite trade-offs between width, reflectivity, sensitivity two modes, reasonable parameters provides nearly optimal performance. Experimental verification biotin-streptavidin as model system...
Stimuli-responsive materials capable of manifesting physical changes in response to environmental signals are valuable tools for use a variety biomedical applications. Herein we describe one such smart glucose-responsive hydrogel material prepared by immobilizing the glucose/galactose binding protein within an acrylamide network. This demonstrates quantitative "accordion"-like dynamic presence glucose. We further show feasibility employing this responsive as gating agent controlled drug...
Nanomaterials have found numerous applications as tunable, remotely controlled platforms for drug delivery, hyperthermia cancer treatment, and various other biomedical applications. The basis the interest lies in their unique properties achieved at nanoscale that can be accessed via remote stimuli. These could then exploited to simultaneously activate secondary systems are not actuatable. In this work, iron oxide nanoparticles encapsulated a bisacrylamide cross-linked polyacrylamide hydrogel...
Abstract Ab initio structure prediction and de novo protein design are two problems at the forefront of research in fields structural biology chemistry. The goal ab proteins is to correctly characterize 3D a using only amino acid sequence as input. De involves production novel sequences that adopt desired fold. In this work, results double‐blind study presented which new method was successfully used predict designed through an experimental approach binary patterned combinatorial libraries...
YiiP is a 32.9-kDa metal transporter found in the plasma membrane of Escherichia coli (Chao, Y., and Fu, D. (2004) J. Biol. Chem. 279, 17173–17180). Here we report determination oligomeric state detergent-lipid micelles membranes. Molecular masses solubilized with dodecyl-, undecyl-, decyl-, or nonyl-β-d-maltoside were measured directly using size-exclusion chromatography coupled laser light-scattering photometry, yielding mass distribution homo-oligomers within narrow range (68.0–68.8 kDa)...