Aims Fibronectin ( FN ) has attracted interest in cancer research, owing to its role tumour progression. The aims of this study were investigate the expression and clinical relevance breast cancer, explore relationship with matrix metalloproteases MMP s) their inhibitors TIMP s). Methods results An immunohistochemical was performed using tumours from 110 patients, tissue arrays specific antibodies against , ‐7, ‐9, ‐11, ‐1, ‐2. indicated that related size, histological grade, ‐9 expression....

Hepatocellular carcinoma (HCC) is in the 10 leading cancer types, being difficult to detect as most of patients who develop this tumor have no symptoms other than those related their long-standing liver disease. The constantly exposed bacterial products, viral infection, alcohol or which may be cause chronic damage, and thus an increasing risk for HCC. Toll-like receptors (TLR) gained extraordinary interest research due role several biological processes such innate immune responses,...

Tumors are infiltrated by macrophages, T and B-lymphocytes, which may favor tumor development promoting angiogenesis, growth invasion. The aim of this study was to investigate the clinical relevance relative amount macrophages (CD68+), T-cells (CD3+) B-cells (CD20+) at invasive front breast carcinomas, expression matrix metalloproteases (MMPs) their inhibitors (TIMPs) either or center. We performed an immunohistochemical counting CD3, CD20 CD68 positive cells front, in 102 carcinomas. Also,...

Inflammatory conditions may promote tumor progression and aggressiveness. In previous reports, we found a group of breast cancer tumors characterized by metalloprotease-11 (MMP-11) expression intratumoral mononuclear inflammatory cells (MICs), which was associated with distant metastasis development. Thus, in the present study evaluated relationship between MMP-11 MICs, development, wide panel factors carcinoma. an initial approach, analyzed 65 inflammation, population classified good or bad...

Although several loci for familial dilated cardiomyopathy (DCM) have been mapped, the origin of a large percentage DCM remains unclear. Mdm2, p53-negative regulator, protects cardiomyocytes from ischemic and reperfusion-induced cell death. Mdm4, homolog inhibits p53 activity in numerous types. It is unknown whether Mdm4 plays role inhibition fully differentiated tissues such as adult this associated with DCM.The conditional knockout heart by use cardiomyocyte-specific Cre (alphaMyHC-Cre)...

The objective of the present work was to evaluate impact phenotype both mononuclear inflammatory cells (MICs) and cancer-associated fibroblast (CAFs) in early breast cancer patients, specifically assessed as their expression MMP/TIMP relative position within tumor (i.e., localization at center or invasive front) occurrence distant metastases.. An immunohistochemical study performed using tissue arrays specific antibodies against matrix metalloproteinase (MMP)−1, −2, −7, −9, −11, −13 −14,...

Toll-like receptors (TLRs) have raised an extraordinary interest in cancer research due to their role tumor progression. By activating the production of several biological factors, TLRs drive inflammatory response and activate adaptive immune system. The aim this study was investigate expression clinical relevance TLR3, TLR4, TLR9 gastric cancer. For purpose, immunohistochemical on specimens from 106 patients with performed using tissue arrays specific antibodies against TLR9. results...

Breast tumors infiltrated by matrix metalloprotease 11 (MMP-11)+ mononuclear inflammatory cells are prone to form metastases; express high levels of interleukin (IL)-1, IL-5, IL-6, IL-17, interferon β (IFNβ) and NFκB; exhibit an increased CD68+/(CD3+CD20+) cell ratio at their invasive front. These factors, which implicated in the crosstalk between microenvironment, may emerge as attractive prognostic factors therapeutic targets.

Abstract Although mutant Ras proteins were originally described as transforming oncoproteins, they induce growth arrest, senescence, and/or differentiation in many cell types. c-Myc is an oncogenic transcription factor that cooperates with cellular transformation and oncogenesis. However, the Myc-Ras relationship largely unknown. Here, we have analyzed effects of on PC12-derived cells (UR61 line), harboring inducible N-Ras oncogene. In these cells, activation induces neuronal-like by a...

Because of the important role in inflammation and tissue regeneration, toll-like receptors (TLR) are likely candidates to mediate effects innate immune system on tumorigenesis. The aim this study was investigate expression clinical relevance TLR colorectal cancer (CRC). expressions TLR3, TLR4, TLR7, TLR9 were analyzed 104 patients with resectable CRC by immunohistochemistry. evaluation consisted measuring overall level each cell type. results showed a direct association between histologic...

The aim of this work was to evaluate the expression and clinical relevance some cytokines in breast carcinomas. An immunohistochemical study using tissue arrays specific antibodies against interleukin 1β (IL-1β), IL-6, IL-10, IL-17, interferon β (IFNβ) nuclear factor kappa B (NFκB) performed 108 Most studied were mainly expressed by cancer cells but also stromal as cancer-associated fibroblasts (CAFs) or mononuclear inflammatory (MICs). Global (score) IL-1β IL-17 positively associated with...

The biological heterogeneity of breast cancer leads to the need for finding new approaches understand mechanisms implicated in progression. tumor stroma appears as a key progression solid tumors towards malignant phenotype. Cancer associated fibroblasts (CAFs) may orchestrate functional "corrupted" which turn helps metastatic spread. In this study, we investigated by real-time PCR, expression 19 factors normal breast-associated (NAFs) and CAFs, were several actions promoting growth, such...

Tumor Necrosis Factor (TNF) interacts with two receptors known as TNFR1 and TNFR2. activation may result in either cell proliferation or death. TNFR2 activates Nuclear Factor-kappaB (NF-kB) c-Jun N-terminal kinase (JNK) which lead to transcriptional of genes related survival. This depends on the binding TNF Receptor Associated 2 (TRAF2) receptor. also induces TRAF2 degradation. In this work we have investigated structural features responsible for inducing degradation studied biological...

There are accumulating epidemiological, experimental, and genetic data supporting that prostate inflammation may contribute to carcinogenesis, several inflammatory-related molecules have been linked tumorigenesis prognosis in tumors. The aim of this study was evaluate tumor expression factors carcinomas their possible relationship with biochemical recurrence (elevation prostate-specific antigen serum levels). An immunohistochemical conducted using tissue microarrays specific antibodies...

The TP73 gene gives rise to transactivation domain-p73 isoforms (TAp73) as well DeltaNp73 variants with a truncated N terminus. Although TAp73alpha and -beta proteins are capable of inducing cell cycle arrest, apoptosis, differentiation, acts in many types dominant-negative repressor p53 TAp73. It has been proposed that p73 is involved myeloid its altered expression leukemic degeneration. However, there little evidence which (TA or DeltaN) expressed during differentiation whether specific...

Degradation of the stromal connective tissue and basement membrane components are key elements in tumor invasion metastasis.Some components, particularly interstitial collagens, very resistant to proteolytic attacks can be degraded by specific proteinases like Matrix Metalloproteinases (MMPs).MMPs also impact on cell behavior vivo as a consequence their ability cleave growth factors, surface receptors, adhesion molecules, or chemokines/cytokines, for stimulating angiogenesis.Different...

Microtubule interfering agents (MIAs) are antitumor drugs that inhibit microtubule dynamics, while kinesin spindle protein (KSP) inhibitors substances block the formation of bipolar during mitosis. All these compounds cause accumulation mitotic cells and subsequently cell death. We used two-dimensional gel electrophoresis (2DE) followed by MALDI-MS analysis to demonstrate MIAs vinblastine (Velban) paclitaxel (Taxol), as well KSP inhibitor S-tritil-L-cysteine, induce phosphorylation annexin...

Abstract An integrated multidisciplinary approach that combined structure‐based drug design, multicomponent reaction synthetic approaches and functional characterization in enzymatic cell assays led to the discovery of new kinesin spindle protein (KSP) inhibitors with antiproliferative activity. A focused library benzimidazoles obtained by a Ugi+Boc removal/cyclization sequence generated low‐micromolar‐range KSP as promising anticancer prototypes. The design studies chemotypes were assessed...