A5007238782- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Ovarian cancer diagnosis and treatment
- Cell Adhesion Molecules Research
- Sirtuins and Resveratrol in Medicine
- Plant Reproductive Biology
- Cancer Mechanisms and Therapy
- Helicobacter pylori-related gastroenterology studies
- Lichen and fungal ecology
- TGF-β signaling in diseases
- Galectins and Cancer Biology
- Nanoparticle-Based Drug Delivery
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Melanoma and MAPK Pathways
- HER2/EGFR in Cancer Research
- Eosinophilic Esophagitis
- Toxin Mechanisms and Immunotoxins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Nuclear Physics and Applications
- Chemokine receptors and signaling
- Endometrial and Cervical Cancer Treatments
University of Otago
2015-2025
Christchurch Hospital
2012-2023
University of Canterbury
2005-2012
ABSTRACT Helicobacter pylori bacteria colonize the human stomach where they stimulate a persistent inflammatory response. H. is considered noninvasive; however, lipopolysaccharide (LPS)-enriched outer membrane vesicles (OMV), continuously shed from surface of this bacterium, are observed within gastric epithelial cells. The mechanism vesicle uptake poorly understood, and study was undertaken to examine roles bacterial VacA cytotoxin LPS in OMV binding cholesterol clathrin-mediated...
Advanced endometrial cancer often shows resistance to clinical chemotherapy although potencies of anticancer drugs in vitro are promising. The disparity suggests that vivo microenvironments not recapitulated by models used for preclinical testing. However, spheroids replicate some important properties tumours vivo. Therefore, the first time, we compared effects doxorubicin and cisplatin on 3D multicellular structures 2D cell monolayers cells.3D were generated culturing cells non-adherent...
Chronic Helicobacter pylori infection is associated with an increased risk of gastric carcinogenesis. These non-invasive bacteria colonize the mucosa and constitutively shed small outer membrane vesicles (OMV). In this study, we investigated direct effect H.pylori OMV on cellular events We observed micronuclei formation in AGS human epithelial cells treated isolated from a toxigenic strain (60190). This was absent 60190v:1 that has mutant vacA , indicating VacA-dependent formation. VacA...
Nanomedicine has advanced the biomedical field with availability of multifunctional nanoparticles (NPs) systems that can target a disease site enabling drug delivery and helping to monitor disease. In this paper, we synthesised gold (AuNPs) an average size 18, 40, 60 80 nm, studied effect size, concentration incubation time on ovarian cancer cells namely, OVCAR5, OVCAR8, SKOV3. The measured by transmission electron microscopy images was slightly smaller than hydrodynamic diameter; ImageJ as...
Emerging multifunctional nanoparticulate formulations take advantage of nano-meter scale size and surface chemistry to work as a therapeutic delivery agent diagnostic tool for non-invasive real-time monitoring using SPCCT imaging technology.
Ovarian cancer poses a significant threat to patients in its advanced stages, often with limited treatment options available. In such cases, palliative management becomes the primary approach maintaining reasonable quality of life. Therefore, administration any medication that can benefit without curative option holds potential. Resveratrol, natural compound known for vitro anticancer activities, has generated contrasting results vivo and human studies. this study, we aimed assess effects...
Key features of advanced ovarian cancer include metastasis via cell clusters in the abdominal cavity and increased chemoresistance. Resveratrol derivatives resveratrol have been shown to antitumour properties. The purpose this study was investigate effect acetyl-resveratrol on 3D aggregates cancer, establish if NF-κB signalling may be a potential target. Poly-HEMA coated wells were used produce two lines, SKOV-3 OVCAR-5. exposed 10, 20 or 30 μM for 2, 4 6 days. Cell growth metabolism...
Resveratrol has aroused significant scientific interest as it been claimed that exhibits a spectrum of health benefits. These include effects an anti-inflammatory and antitumour compound. The purpose this study was to investigate compare any potential antigrowth resveratrol two its derivatives, acetyl-resveratrol polydatin, on 3D cell aggregates the EGFR/Her-2 positive negative ovarian cancer lines SKOV-3 OVCAR-8, respectively. Results showed reduced growth in OVCAR-8 dose-dependant manner....
Abstract To streamline the drug discovery pipeline, there is a pressing need for preclinical models which replicate complexity and scale of native tumors. While have been advancements in formation microscale tumor units, these are cell‐line dependent, time‐consuming not improved clinical trial success rates. In this study, two methods generating 3D microenvironments compared, rapidly fabricated hydrogel microspheres traditional cell‐dense spheroids. These modules then bioassembled into...
Most ovarian cancer patients are diagnosed with advanced stage disease, which becomes unresponsive to chemotherapeutic treatments. The PI3K/AKT/mTOR and the RAS/RAF/MEK/ERK kinase signaling pathways attractive targets for potential therapeutic inhibitors, due high frequency of mutations PTEN, PIK3CA, KRAS BRAF in several subtypes. However, monotherapies targeting one these have shown modest effects clinical trials. This limited efficacy agents could be upregulation increased via adjacent...
Advanced ovarian cancer is often a fatal disease as chemotherapeutic drugs have limited effectiveness. Better targeted therapy needed to improve the survival and quality of life for these women. Receptor tyrosine kinases including EGFR, Her-2 c-Met are associated with poor prognosis in cancer. Therefore, co-activation receptors may be crucial growth promoting activity. In this study, we explored effect combining two small molecule inhibitors that target EGFR/Her-2 receptor cell lines. The...
Abstract Cutaneous melanoma is a highly invasive, heterogeneous and treatment resistant cancer. It’s ability to dynamically shift between transcriptional states or phenotypes results in an adaptive cell plasticity that may drive cancer invasion the development of therapy resistance. The expression peroxidasin (PXDN), extracellular matrix peroxidase, has been proposed be associated with invasive metastatic phenotype. We have confirmed this association by analysing transcriptomes 70 lines...
The structure and function of membrane–wall attachment sites in walled cells, how these relate to animal focal adhesions, is an area that poorly understood. In view this, we investigated attachments form upon plasmolysis, respond peptides disrupt adhesions. degree cytoplasmic disruption during plasmolysis was also investigated. Upon hyperosmotic challenge, the protoplast hyphae oomycete Achlya bisexualis typically retracted incompletely due attachments. inclusion, plasmolysing solution,...