Shrinkhala Manandhar

ORCID: 0000-0002-9997-8900
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About
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Research Areas
  • Plant Pathogens and Fungal Diseases
  • Banana Cultivation and Research
  • Plant Disease Management Techniques
  • Nematode management and characterization studies
  • Plant Pathogens and Resistance
  • Plant-Microbe Interactions and Immunity
  • Plant tissue culture and regeneration
  • SARS-CoV-2 detection and testing
  • Plant Virus Research Studies
  • Plant and soil sciences
  • Infection Control and Ventilation
  • Plant Pathogenic Bacteria Studies
  • Mycorrhizal Fungi and Plant Interactions
  • Dental Research and COVID-19
  • Mycotoxins in Agriculture and Food
  • Bioeconomy and Sustainability Development
  • Agriculture, Plant Science, Crop Management

Nepal Agricultural Research Council
2016-2023

Soil borne pathogenic fungi are of major concern in agriculture which significantly decreases the plant yield. Chemically controlled imposes environmental threats potentially dangerous to humans as well other animals. Thus, application biological methods disease control is more effective alternative technique. This study was carried out isolate Trichoderma species from soil sample and assess its vitro biocontrol efficacy against fungal pathogens viz. Sclerotium rolfsii, Sclerotionia...

10.3126/njb.v5i1.18492 article EN cc-by Nepal Journal of Biotechnology 2017-12-31

Fusarium wilt of banana (FWB), caused by oxysporum f. sp. cubense (Foc), is the most important constraint industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on Malbhog cultivar in past several years. However, disease has not officially reported yet, and consequently, little known about pathogen present across country. this study, we characterized 13 fungal strains isolated from plants (Silk, AAB) showing symptoms similar to plantations Nepal. All were typed...

10.3390/jof9020208 article EN cc-by Journal of Fungi 2023-02-05

Biocontrol is an important aspect of disease management for plant pathogens, especially the soil borne fungi. Trichoderma species most exploited biocontrol agent in recent years. The specific nature a well-known fact and hence native isolates should be more emphasized control pathogens. Fifty samples from rhizosphere various agricultural crops were collected isolation sp. Ten tested dual culture with pathogens Fusarium solani, Rhizoctonia solani Sclerotinia sclerotiorum vitro assay. All test...

10.3126/jnarc.v5i1.23810 article EN cc-by-nc Journal of Nepal Agricultural Research Council 2019-04-28

Pot experiment was conducted to find out the competence of some commercial product Trichoderma spp. on management root knot nematode in tomato crop. The RCBD having seven treatments six different including one control three replications. Each treatment had five pots. Twenty days old seedling Shrijana hybrid transplanted and inoculated with 1500 eggs Meloidogyne soil after transplanting seedlings. spore suspensions adjusted 1 × 106 spores/ml also per pot 30 ml week inoculation. same...

10.3126/jpps.v8i1.56447 article EN cc-by-nc Journal of the Plant Protection Society 2023-07-10

Bacterial wilt caused by Ralstonia solanacearum E.F. Smith is one of the destructive diseases solanaceous vegetables specially tomato (Lycopersicon esculentum L.) and eggplant (Solanum melongena L.). Experiments were conducted to determine biovar types existing among strains or isolates Nepal variation in virulence some belonging solanaceae family. A total 39 infecting tomato, eggplant, chilli potato collected from different parts analyzed for on basis 3 disaccharides hexose alcohols...

10.3126/jnarc.v2i0.16117 article EN cc-by-nc Journal of Nepal Agricultural Research Council 2016-12-30

Phytophthora blight (PB) caused by capsici Leonian is an economically important disease of pepper in Nepal. Experiments were conducted epiphytotic conditions at Luvu, Lalitpur where severe outbreak PB used to occur. The experiment was designed randomized complete block with three replications. Three isolates Trichoderma harzianum viz, T. (T22), (T69), and one isolate asperellum, tested for their efficacy under the field compared chemical fungicides, copper oxychloride fluazinam two years,...

10.3126/nh.v14i1.30600 article EN Nepalese Horticulture/Nepalese horticulture 2020-08-26

Trichoderma is a well documented biocontrol agent of fungus origin being used for the control various plant pathogens as people are aware detrimental effects chemical pesticides. In Nepal also, agents gaining popularity and there several products in different trade names available market with label that they can be within six months from date manufacture. However, it very essential to know fact these microbial contain living micro-organisms therefore conditions storage long term viability...

10.3126/jpps.v5i0.47125 article EN cc-by-nc Journal of the Plant Protection Society 2018-12-31

Banana is affected by a wide number of diseases, which, Fusarium wilt caused oxysporum f.sp. cubense has played major role in devastating Malbhog (Silk) banana plantations Nepal. The objective this study was to evaluate the commonly available fungicides (Carbendazim 50%, Fenamidone 10%+ Mancozeb Chlorothalonil and biocontrol agents (Trichoderma sps., Bacillus thuringiensis Pseudomonas species) for their efficacy against Foc. effectiveness Foc examined screenhouse. experiment laid out...

10.3126/aej.v24i01.58195 article EN Deleted Journal 2023-06-30

A total of 211 soil samples were collected in different crop fields from 10 districts. Out samples, 137 diagnosed for plant parasitic nematodes. The the cruciferous and solanaceous crops. All together twelve nematodes identified. identified genera are Meloidogyne, Helicotylenchus, Pratylenchus, Tylenchus, Tylenchorhynchus, Hoplolaimus, Belonolaimus, Criconemoides, Aphelenchoides, Hirschmanniella, Longidorus Rotylenchulus. They found either single or more than one genus a sample. It means...

10.30574/wjarr.2023.17.3.0360 article EN cc-by-nc-sa World Journal of Advanced Research and Reviews 2023-03-26

Diseases are one of the major constraints on cultivation crops and reduce production productivity. Accurate disease diagnosis proper identification is first steps management strategy. The activities carrying out help to know distribution diseases in country, explore new outbreak its epidemiology, provide information for support research area prioritization. During fiscal year 2070/71, 252 different samples vegetable were received from various locations sources diagnosis. Examination such...

10.3126/jpps.v5i0.47121 article EN cc-by-nc Journal of the Plant Protection Society 2018-12-31

We communicate briefly about the innovation of protective gear (such as Hazmat suit) and respirator mask that can be used by health care professionals mouth nose protection tools against penetration microscopic particles containing highly contagious species into body. As a research development support to local entrepreneurs willing come up with innovative products, structural some surface functional properties polymeric materials available in market were analyzed comparison commercially...

10.3126/asta.v1i1.30272 article EN Applied Science and Technology Annals 2020-06-30

Rice blast disease caused by Pyricularia oryzae is a major threat to the farmers as severity may lead upto 80% crop loss in severe conditions. A laboratory experiment was conducted at National Plant Pathology Research center of NARC, Khumaltar,Lalitpur for testing efficacy different fungicides adopting poisoned food method against pathogen causing rice disease. The carried out completely randomized design with 10 treatments including control following 3 replications each. Two concentrations...

10.22161/ijeab.63.17 article EN International Journal of Environment Agriculture and Biotechnology 2021-01-01
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