<ns4:p>Background <ns4:italic>Trichoderma</ns4:italic> spp. hold significant potential as biocontrol agents in agriculture due to their antagonistic properties against plant pathogens. The study aimed characterize and identify isolates from rhizospheric regions of vegetable crops. Methods In this study, were collected soil samples crops different ecological zones selected for comprehensive morphological molecular characterization. visually assessed colony color, growth pattern, aerial...

Since the emergence of Ug99 wheat stem rust in Uganda 1998 (Pretorius et al. 2000), threat movement into South Asia has been a concern due to long-distance dispersal capacity airborne spores (Brown and Hovmøller 2002; Singh 2008; Meyer 2017). Increased preparedness by comprehensive surveillance efforts development deployment resistant cultivars advance an incursion one success stories Borlaug Global Rust Initiative (Sharma 2013). In November 2023, off-season survey was conducted Marpha,...

<ns3:p>Background <ns3:italic>Trichoderma</ns3:italic> spp. hold significant potential as biocontrol agents in agriculture due to their antagonistic properties against plant pathogens. The study aimed characterize and identify isolates from rhizospheric regions of vegetable crops. Methods In this study, were collected soil samples crops different ecological zones selected for comprehensive morphological molecular characterization. visually assessed colony color, growth pattern, aerial...

Abstract Wheat contributes one‐fifth of the global food supply with an estimated 29% production in low and lower‐middle income countries. As expands across southern Asia, yields are often negatively impacted by outbreaks fungal rust diseases. A wheat early warning advisory system comprising surveillance, near real‐time disease risk forecasts dissemination has been established two target countries South including Nepal Bangladesh. However, as spores can be aerially transmitted over long...

Fusarium wilt of banana (FWB), caused by oxysporum f. sp. cubense (Foc), is the most important constraint industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on Malbhog cultivar in past several years. However, disease has not officially reported yet, and consequently, little known about pathogen present across country. this study, we characterized 13 fungal strains isolated from plants (Silk, AAB) showing symptoms similar to plantations Nepal. All were typed...

The root-knot nematodes (Meloidogyne spp) are difficult to manage once established in the field because of their wide host range, and soil-borne nature. Thus, aim present study was examine use resistant root stock wild brinjal (Solanum sisymbriifolium) reduce loss caused by on tomato. For management nematodes, grafted plant with tested under farmers’ conditions at Hemza Kaski district. Grafted non-grafted plants were produced nematode-free soil. Around three week-old tomato transplanted four...

Rice False Smut (RFS) caused by Ustilaginoidea virens is a major emerging disease of rice due to expanded area hybrid cultivars, increasing use nitrogenous fertilizers and change in climate. Due the incidences this across globe, there pressing need develop techniques for false smut management. The application fungicides with high efficiency, low toxicity, residue currently best option control RFS. Therefore, current research was conducted determine effectiveness manage experiments were...

<ns4:p>Background <ns4:italic>Trichoderma</ns4:italic> spp. hold significant potential as biocontrol agents in agriculture due to their antagonistic properties against plant pathogens. The study aimed characterize and identify isolates from rhizospheric regions of vegetable crops. Methods In this study, were collected soil samples crops different ecological zones selected for comprehensive morphological molecular characterization. visually assessed colony color, growth pattern, aerial...

Biocontrol is an important aspect of disease management for plant pathogens, especially the soil borne fungi. Trichoderma species most exploited biocontrol agent in recent years. The specific nature a well-known fact and hence native isolates should be more emphasized control pathogens. Fifty samples from rhizosphere various agricultural crops were collected isolation sp. Ten tested dual culture with pathogens Fusarium solani, Rhizoctonia solani Sclerotinia sclerotiorum vitro assay. All test...

The western Himalayan region in Pakistan has been shown to be the center of diversity Puccinia striiformis; however, little is known about its genetic relations with eastern part Himalayas. We studied structure P. striiformis from Nepal (35 isolates) and Bhutan (31 comparison 81 Pakistani samples collected during 2015 2016, through microsatellite genotyping. Genetic analyses revealed a recombinant highly diverse population Pakistan, Bhutan, Nepal. A high level genotypic (>0.90) was observed...

Gray leaf spot (GLS) is an important and destructive disease of maize in the hills Nepal. The occurrence this recorded for first time country 2006. Several genotypes supposed to be resistant gray SARMP Zimbabwe CIAT Colombia were evaluated together with other varieties observation nursery conducted at farmer’s fields as well Khumaltar (NARC research station Kathmandu Valley) during 2008 2009. Ten out 28 identified moderately GLS. incidence was higher on open pollinated maize. severity GLS...

Spot blotch disease of wheat caused by Bipolaris sorokiniana; is one the most concerning in warm and humid regions Nepal due to its widespread prevalence increasing severity. An in-vitro experiment was conducted evaluate efficacy different botanicals fungicides against sorokiniana, National Plant Pathology Research Centre’s laboratory at Khumaltar, Lalitpur 2019. The done completely randomized design with three replications poison food technique. Five viz. neem, garlic, eucalyptus, bojho...

Pot experiment was conducted to find out the competence of some commercial product Trichoderma spp. on management root knot nematode in tomato crop. The RCBD having seven treatments six different including one control three replications. Each treatment had five pots. Twenty days old seedling Shrijana hybrid transplanted and inoculated with 1500 eggs Meloidogyne soil after transplanting seedlings. spore suspensions adjusted 1 × 106 spores/ml also per pot 30 ml week inoculation. same...

Abstract Wheat is a staple crop in Nepal and the third major cereal grown across country. To improve productivity increase number of farmers growing wheat, Agricultural Research Council (NARC), since 1962, has been releasing new wheat varieties with higher disease resistance. Accurate identification farmer's fields critical for assessing adoption levels impact varieties. This understanding can change landscape market overall vulnerability to diseases abiotic stresses. Current methods that...

Tomato (Solanum lycopersicum) is one of the most important vegetable crops in Nepal and grown high tunnels open fields (Rawal et al., 2017). In June 2022, tomato plants having blighted lesions with concentric circles, yellow halos, severely defoliated leaflets, an incidence c. 80%, were obtained from growers Panauti area Kabrepalanchok district central hills Nepal, 3 ha. Samples examined under a microscope tape mounting slightly curved to cylindrical, multiple pseudoseptate conidia...

HomePlant DiseaseVol. 106, No. 11First Report of Strawberry Polerovirus 1 in Nepal PreviousNext DISEASE NOTE OPENOpen Access licenseFirst NepalH.-R. Kwak, H.-S. Byun, S.-B. Hong, Choi, H. K. Manandhar, R. D. Timila, S. Joshi, N. Rokaya, Baidya, and P. HumagainH.-R. Kwak†Corresponding authors: H.-R. Kwak; E-mail Address: [email protected], Manandhar; protected]https://orcid.org/0000-0002-1852-9776Crop Protection Division, National Institute Agricultural Sciences, Rural Development...

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. is considered as one of the major threats to rice production in Nepal. To identify effective control measures, an experiment was done a randomized complete block design with three replications farmer's field at Bhaktapur, during summer seasons 2018 and 2019. Three plants extracts; 1% Neem extract, 2% Asuro Titepati extract along two fungicides viz. 0.1% Agricin 0.2% Blitox 50, bio-control agent 1%Kisan Pseudomonas were used...

Lentil collar rot disease caused by Sclerotium rolfsii Sacc. is an important causing significant yield loss in Nepal. Seven Trichoderma spp. isolates collected from different regions of Nepal were tested for their capacity to inhibit mycelial growth in-vitro condition. Experiment was conducted Completely Randomized Design with four replications the plant pathology laboratory Grain Legumes Research Program, Khajura, Banke during 2020. Dual culture method performed assess efficacy isolates....

Abstract Wheat stripe/yellow rust (WYR), caused by Puccinia striiformis f. sp. tritici ( Pst ), is a major constraint in global wheat production. A set of 766 hexaploid synthetic lines, including primary crosses Triticum turgidum x Aegilops tauschii and their derivatives, were screened artificially inoculated field nurseries for three seasons. From this set, core 94 non-lodging lines with unique pedigrees resistance to that was consistent across years established. The tested adult plant...

Chemical use in agricultural farming recent years has led to many threats concerning the environment and human health. Trichoderma spp. been used as a biocontrol agent is gaining popularity years. Integrated of with compatible chemicals one disease management strategy which would aid immediate action on plant pathogens provide control over long term well. In vitro compatibility test five isolates thirteen different chemical fungicides at two concentrations was carried out laboratory Plant...

Cyanobacteria offer an economically and ecologically sound alternative to chemical fertilizers for realizing the ultimate goal of increased productivity, especially in rice cultivation.There is however, a growing concern about adverse effects indiscriminate use on soil productivity environmental quality.A comparative study between NR activity biomass six Anabaena strains isolated from paddy field soils Ganjam district presence pH, temperature, copper& molybdenum, NaNO3, NH4Cl, Urea being...

Blast, caused by Pyriculariagrisea (Sacc.) is the most destructive disease of rice in Nepal. To identify sources leaf blast resistance genotypes, a field experiment was conducted under natural epiphytotic condition at National Plant Pathology Research Centre (NPPRC), Khumaltar, Lalitpur, Nepal during summer season 2018 and 2019.A total 128 genotypes 291during 2019 including resistant check (Sabitri) susceptible (Shankharika/Mansuli) were tested. Field single rod row design. Leaf assessment...