ABSTRACT The global rise in obesity is closely associated with the increasing prevalence of nonalcoholic fatty liver disease (NAFLD) and metabolic syndromes, posing significant health challenges. This study explored ameliorative effects Ophiocordyceps sinensis mycelia (TCM‐NA01 formula: 1.4 mg adenosine 55.2 polysaccharide/capsule) on hepatosteatosis development a high‐fat diet (HFD)‐fed mice. TCM‐NA01 supplementation significantly reduced ( p < 0.05) body weight, adipose tissue, serum...

Abstract The effects of heat shock (HS) on the nucleus and cytoskeleton in vitro matured pig oocytes were examined this study. Porcine cumulus–oocyte complexes (COCs) aspirated from 3 to 6 mm diameter follicles subjected standard maturation (IVM) procedures for 42 hr. In Experiment 1, IVM‐derived then randomly allocated different HS treatments at 41.5°C 0 (control, C0h, n = 101), 1 (HS1h, 113), 2 (HS2h, 104), 4 hr (HS4h, 111), respectively. An additional control group was cultured without...

Via an assay using Amino Acid Analyzer, pepsin-digested chicken liver hydrolysates (CLHs) contain taurine (365.57 ± 39.04 mg/100 g), carnosine (14.03 1.98 and anserine (151.58 27.82 g). This study aimed to evaluate whether CLHs could alleviate thioacetamide (TAA)-induced fibrosis. A dose of 100 mg TAA/kg BW significantly increased serum damage indices cytokine contents. Cell infiltration monocytes/macrophages in livers TAA-treated rats were illustrated by the H&E staining immunohistochemical...

In the present study, we investigated effects of Sonic hedgehog (Shh) protein on porcine oocyte maturation and early embryo development. Immunohistochemistry showed activation Shh signalling in cumulus–oocyte complexes (COCs), as reflected by Patched (Ptc), Smoothened (Smo) Gli1 expression oocytes, cumulus cells granulosa cells, particularly those small follicles (<2 mm diameter). Western blot analysis Smo COCs denuded oocytes derived from medium (3–7 mm)-sized follicles. Small contained...

Hyperlipidemia of hamsters was induced by high-fat/cholesterol diets formulated the addition coconut oil (CO), butter (BU), and flaxseed (FX). Lower (p < 0.05) serum lipids, liver size, hepatic cholesterol triacylglycerol contents were observed in FX group compared to both CO BU groups. The damage indices [glutamic oxaloacetic transaminase (GOT) glutamic pyruvic (GPT) values] lower than those groups, which may result from higher glutathione (GSH) levels a tendency toward malondialdehyde...

Recent investigations have demonstrated that carotenoid extract of Dunaliella salina alga (Alga) contains abundant β-carotene and has good anti-inflammatory activities. Murine macrophage (RAW264.7 cells) was used to establish as an in vitro model pseudorabies virus-induced reactive oxygen species (ROS) response. In this study, antioxidant activities Alga were measured based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, trolox equivalent capacity assays, reducing power, ROS...

The aim of the present study was to improve quality handmade cloned porcine embryos by multiple embryo aggregations. Embryos derived from aggregation three (3×) had a better blastocyst rate than control (1×) (73.6% vs 35.1%, respectively; P<0.05), but did not differ those produced two (2×; 63.0%). Total cell numbers differed among treatments (P<0.05), with greatest (126) in 3× group and lowest (55) group. ratio inner mass:total number comparable 2× groups (25.1% 26.1%, respectively)...

Abstract Lonicerae japonicae ( L . ) flos is a medical and food homology herb. This study investigated the phenolic acid flavonoid contents in water extract solution (LJWES) preventive effects of LJWES against liver fibrogenesis via FL83B cells rats. contains many polyphenols, such as chlorogenic acid, morin, epicatechin. increased cell viability decreased cytotoxicity thioacetamide (TAA)‐treated (75 mM) p &lt; .05). .05) gene expressions Tnf‐α , Tnfr1 Bax cytochrome c but upregulated Bcl‐2...

Abstract Thioacetamide (TAA), usually used as a fungicide to control the decay of citrus products, itself is not toxic liver, but its intermediates are able increase oxidative stress in livers and further cause fibrosis. Ophiocordyceps sinensis mycelium (OSM) which contains 10% polysaccharides 0.25% adenosine decreased ( P &lt; 0.05) lipid accumulation increased antioxidative capacity thioacetamide (TAA) injected rats. Meanwhile, liver sizes, serum alanine transaminase (AST) aspartate (ALT)...

Our patented protease A–digested crude chalaza hydrolysates (CCH) show antioxidant abilities in vitro. The prophylactic effects of CCH on cognitive dysfunction and brain oxidative damages were investigated via a D-galactose (DG)–injected mouse model this study. Fifty-four mice randomly divided into the following: (1) CON, 0.1 mL 0.9% saline (subcutaneous injection [SC] back)+distilled water (oral gavage); (2) DG, 100 mg/kg BW/day (Bio-Serv Co., Flemington, NJ, USA) (SC (3) DG_LCH, back) + 50...

Four-hundred metric-ton chalazae are produced annually from the liquid-egg processing and always cause a heavy burden due to handling cost in Taiwan. After were hydrolyzed by protease A, amounts of hydrophobic, aromatic, branched-chain amino acids, as well anserine dramatically increased. This study was understand antifibrogenic effects A-digested crude chalaza hydrolysates (CCH-As) on livers thioacetamide (TAA) treated rats. CCH-As improved (P< 0.05) growth performance, serum liver damage...

In this study, we determined the expression and activation of p38 MAPK in matured porcine oocytes subjected to heat shock (HS). When MII were heated, only phosphorylated levels relative total decreased (P < 0.01) after HS, but no clear relationship with HS treatments was observed ERK, JNK p90rsk expressions oocytes. To confirm oocytes, immunocytochemical staining performed localize its distribution ooplasm, results largely consistent previous Western blot analyses. Moreover, when co-cultured...

Purpose: We previously reported miR-328-3p as a novel risk factor for myopia through genetic association study of the PAX6 gene. In present study, we first explored effects on other myopia-related genes, and then tested whether anti-miR-328-3p may be used control. Methods: The luciferase report assay transient transfection were to confirm target genes. chromatin immunoprecipitation (ChIP) was investigate retinoic acid receptor promoter. Mice pigmented rabbits induced have by form deprivation...

The purpose of this study was to investigate the protective role orally administered taurine against diabetic retinal changes via electroretinogram (ERG) and histology on rabbits. Rabbits were randomly assigned into groups: Group I (vehicle administration only); II (diabetes: induced by 100 mg/kg alloxan injection); III (diabetes fed with 200 taurine); IV 400 taurine). body weight blood glucose levels rabbits monitored weekly. ERG measured weeks 5 15. Retinal analyzed in end experiment....