A5089192515- Ocular Surface and Contact Lens
- Cancer-related Molecular Pathways
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Gout, Hyperuricemia, Uric Acid
- Fungal Biology and Applications
- Wnt/β-catenin signaling in development and cancer
- Ophthalmology and Visual Impairment Studies
- Corneal Surgery and Treatments
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Connexins and lens biology
- Cancer-related gene regulation
- Cancer Research and Treatments
- Bee Products Chemical Analysis
- Multiple Myeloma Research and Treatments
- interferon and immune responses
- Heme Oxygenase-1 and Carbon Monoxide
- Ginger and Zingiberaceae research
- Allergic Rhinitis and Sensitization
- Developmental Biology and Gene Regulation
- Retinoids in leukemia and cellular processes
- Influenza Virus Research Studies
- Ubiquitin and proteasome pathways
Chung Shan Medical University
2016-2025
Chung Shan Medical University Hospital
2019-2025
Chengdu University of Traditional Chinese Medicine
2024
City of Hope
2018-2023
Beckman Research Institute
2018-2021
City Of Hope National Medical Center
2004-2019
University of Southern California
2018
Stony Brook University
2017
Thomas Jefferson University
1992-1994
National Institutes of Health
1991
The ability of p53 to activate transcription from specific sequences suggests that genes induced by may mediate its biological role as a tumor suppressor. Using subtractive hybridization approach, we identified gene, named WAF1, whose induction was associated with wild-type but not mutant gene expression in human brain cell line. WAF1 localized chromosome 6p21.2, and sequence, structure, activation conserved rodents. Introduction cDNA suppressed the growth brain, lung, colon cells culture....
Conditional expression of wild-type (wt) p53 protein in a glioblastoma tumor cell line has been shown to be growth inhibitory. We have now more precisely localized the position cycle where arrest occurs. show that occurs prior or near restriction point late G1 phase cycle. The effect wt on four immediate-early genes (c-FOS, c-JUN, JUN-B, and c-MYC), one delayed-early gene (ornithine decarboxylase), two late-G1/S-phase (B-MYB DNA polymerase alpha) was also examined. Of this subset response...
The p53 gene is a frequent target of mutation in wide variety human cancers. Previously, it was reported that conditional expression wild-type protein cell line (GM47.23) derived from glioblastoma multiform tumor had negative effect on proliferation. We have now investigated the induction this has proliferating-cell nuclear antigen gene. encodes an auxiliary factor DNA polymerase delta and part replication machinery cell. show inhibition cycle progression into S-phase after accompanied by...
To investigate the efficacy and safety of three
Overexpression of wild-type p53 protein has been shown to induce arrest in the G1 stage cell cycle and transactivate expression gene that encodes 21-kDa Waf1/Cip1 protein, a potent inhibitor cyclin-dependent kinase activity. p53-dependent is accompanied by decreased B-myb gene, relative c-myb cellular oncogene. In this study we show required for cells progress from into S phase high levels ectopic uncoupled regulation rescues p53-induced even presence transactivation inhibition cyclin E/Cdk2...
Abstract Background Hedgehog signalling has been implicated in prostate tumorigenesis human subjects and mouse models, but its effects on transforming normal basal/stem cells toward malignant cancer stem remain poorly understood. Methods We produced pCX-shh-IG mice that overexpress protein persistently adult prostates, allowing for elucidation of the mechanism during initiation progression. Various markers were used to characterize confirm transformation into under influence overexpression....
Background: Although canonical Wnt signaling is known to promote tumorigenesis in pancreatic ductal adenocarcinoma (PDAC), a cancer driven principally by mutant K-Ras, the detailed molecular mechanisms which effector β-catenin regulates such are largely unknown. We have previously demonstrated that β-catenin’s differential usage of Kat3 transcriptional coactivator cyclic AMP-response element binding protein-binding protein (CBP) over its highly homologous p300 increases self-renewal and...
Purpose: Type 2 diabetes mellitus (T2DM) leads to diabetic retinopathy (DR) and hepatic impairments. The potential mutual interaction the intermediator between these two injuries are not well elucidated. Both retina liver involved in vitamin A metabolism, suggesting a involvement of its metabolites this interaction. This study aimed elucidate impact either DR or impairment on pathogenesis status each during injury progression. Methods: streptozotocin (STZ)–high-fat diet (HFD)-induced T2DM...
Several pieces of epidemiologic evidence have indicated PM2.5 (particulate matter with a diameter 2.5 μm or less) as causing factor allergic conjunctivitis, but without experimental elucidation mechanism. In the present study, in solution was directly applied to mouse ocular surface elucidate whether might cause and its underlying mechanisms were analyzed.ICR mice challenged for 18 consecutive days eye drops containing at 3.2, 6.4, 12.8 mg/mL 0.9% NaCl saline, along controls prepared...
In the present study, we investigated effects of Sonic hedgehog (Shh) protein on porcine oocyte maturation and early embryo development. Immunohistochemistry showed activation Shh signalling in cumulus–oocyte complexes (COCs), as reflected by Patched (Ptc), Smoothened (Smo) Gli1 expression oocytes, cumulus cells granulosa cells, particularly those small follicles (<2 mm diameter). Western blot analysis Smo COCs denuded oocytes derived from medium (3–7 mm)-sized follicles. Small contained...
Dry eye is a complicated ocular surface disease that causes discomfort, visual disturbance, and frequently observed damage. Emerging hypotheses suggest probiotics may help relieve dry symptoms by modulating inflammation oxidative stress. This study aimed to investigate the therapeutic effects of
Objective: Cordyceps cicadae, a medicinal fungus, is assessed as having many functions: anti-cancer, anti-fatigue, anti-aging, immune-boosting, renal and liver protection. Since the industrial production of C. cicadae mycelium consistently manufactures bioactive compounds superior to wild fruiting bodies, there need confirm toxicity liquid fermented mycelium. Studies showed evaluation in animal models, but safety reports clinical studies are scarce. As such, assessment oral...
Purpose.: This study investigated the effects of dietary α-lipoic acid (α-LA) against ultraviolet B (UVB)-induced corneal and conjunctival degeneration in a mouse model. Methods.: Female CBA mice were randomly divided into five groups, including blank control, UVB without α-LA, with α-LA at 1, 10, 100 mg/kg body weight. Following exposure, surfaces assessed along immunohistochemistry for nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), malondialdehyde (MDA) accumulation, P63+ basal cell...
Over 400 million years ago, the evolution of vertebrates gave rise to a life cycle in which organism began live longer particularly as an adult. To accommodate such lifespan, underwent adaptation, developing mechanism for long-lived cellular homeostasis. This adaptation required population relatively quiescent somatic stem cells (SSCs) along with more proliferative differentiated daughter cell population, and was necessary safeguard genetic attributes SSCs were endowed. Intriguingly, cAMP...
Background: The ancient and highly evolutionarily conserved Wnt signaling pathway is critical in nearly all tissues organs for an organism to develop normally from embryo through adult. generally parsed into “canonical” or Wnt-β-catenin-dependent “non-canonical” β-catenin-independent signaling. Even though designating as either canonical noncanonical allows easier conceptual discourse about this pathway, fact non-canonical crosstalk regulates complex nonlinear networks. Objective: In...
Differential usage of Kat3 coactivators, CBP and p300, by β-catenin is a fundamental regulatory mechanism in stem cell maintenance initiation differentiation repair. Based upon our earlier pharmacologic studies, p300 serine 89 (S89) critical for controlling differential coactivator via post-translational phosphorylation stem/progenitor populations, appears to be target number kinase cascades. To further investigate mechanisms signal integration effected this domain, we generated S89A...