Abstract Background Several studies have demonstrated that iDAScore is more accurate in predicting pregnancy outcomes cycles without preimplantation genetic testing for aneuploidy (PGT-A) compared to KIDScore and the Gardner criteria. However, effectiveness of with PGT-A has not been thoroughly investigated. Therefore, this study aims assess association between artificial intelligence (AI)-based (version 1.0) single-embryo transfer (SET) PGT-A. Methods This retrospective was approved by...

Couples with unexplained infertility (UI) tend to have low fertilization rates current IVF procedures. Here, we attempted identify spermatozoa apoptotic markers in couples UI and unsuccessful intrauterine insemination (IUI) investigated the efficiency benefit of magnetic-activated cell sorting (MACS) for sperm preparation such patients. Sixty two IUI failures were recruited. The prepared by conventional density gradient centrifugation (DGC) divided into aliquots. One aliquot was used as a...

Is the relative telomere length in cumulus cells associated with embryo quality and subject's age? The at time of oocyte collection may be a new potential biomarker for selecting highly competent oocytes good embryos. Telomeres play central roles aging determining cell fate. In mammalian ovarian follicles, maturing are nurtured supported by surrounding somatic cells, mural granulosa cells. A total 350 oocyte–cumulus complex samples were collected from 80 IVF cycles prospectively recruited...

Current semen analysis still commonly depends on a manual microscopy method in clinical laboratories worldwide. However, some of the major disadvantages this technique are that it is labour-intensive, subjective, laboratory-based and time-consuming. Although computer-assisted analysers (CASAs) have enabled partial automation routine analysis, they lack wider acceptance due to their complicated operation. Therefore, development an accessible, rapid standardised for urgently needed. Here, we...

Recently, reports showed that embryos identified as mosaic after preimplantation genetic testing for aneuploid (PGT-A) could result in live birth with lower pregnancy and higher loss rates compared euploid embryos. However, the effects of mosaicism level on reproductive outcomes remain controversial. This study aimed to examine outcomes. Single embryo transfer was offered 108 women who only had Mosaic were labeled by utilizing next generation sequencing (NGS) based PGT-A day 5/6...

: To evaluate the effect of intrauterine infusion and hysteroscopic injection autologous platelet-rich plasma (PRP) in patients with a persistent thin endometrium (EM) undergoing euploid frozen embryo transfer (EFET) cycles.

Blastocysts were cryopreserved by a new two-step ultra-rapid cooling in super-cooled liquid nitrogen (-205 degrees C).There 308 mouse blastocysts collected from fertile B6CBF1 mice and 249 human 51 couples treated with IVF. The Vit-Master cryoloops after treatment 50 100% vitrification solution (VS) for 2 min 30 s, respectively. VS was composed of 20% ethylene glycol, dimethylsulphoxide 0.5 mol/l sucrose tubular fluid medium serum albumin. embryos warmed 0.25 0.125 3 min, survival observed...

This study was designed to assess the capability of ovarian reserve markers, including baseline FSH levels, anti-Müllerian hormone (AMH) and antral follicle count (AFC), as predictors live births during IVF cycles, especially for infertile couples with advanced maternal age and/or male factors.A prospective cohort 336 first IVF/ICSI cycles undergoing a long protocol GnRH agonist investigated. Patients endocrine disorders or unilateral ovaries were excluded.Among tests, AMH had greater area...

This retrospective analysis compared the efficiency of gonadotropin- releasing hormone (GnRH) antagonist (GnRH-ant) protocol and GnRH agonist long (GnRH-a) for patients with diminished ovarian reserve (DOR). A total 1,233 DOR (anti-Mullerian <1.1 ng/mL) were recruited this case-control study. They divided into two groups according to female age. Younger assigned POSEIDON group3 (PG3: age ≤35 years); older group 4 (PG4: >35 years). All underwent controlled stimulation fresh embryo transfer...

This study evaluated the effectiveness of intraovarian platelet-rich plasma (PRP) injection in improving ovarian response and embryo quality IVF patients with poor previous controlled hyperstimulation (COH) cycles. 74 participated, 30 control group 44 PRP group. was injected during follicular phase for The completed two COH cycles, while underwent cycles before after injection. In first cycle, there were no significant differences between groups. However, second showed improvements: number...

Background Matrix metalloproteinases (MMPs) and tissue inhibitors of (TIMPs) are critical regulators extracellular matrix (ECM) proteolysis play a pivotal role in trophoblast invasion during embryo implantation. This study aimed to investigate the effects single-nucleotide polymorphisms (SNPs) MMP TIMP genes on clinical outcomes women undergoing vitro fertilization (IVF). Methods retroprospective included 1014 their first fresh IVF cycle without donor eggs at Lee Women’s Hospital between...

The percentage of peripheral CD56+CD16+ NK cells in the early follicular phase on days 2-3 menstrual cycle repeated implantation failure (RIF) patients was used to evaluate impact intravenous immunoglobulin (IVIG) ART cycles. A total 283 with RIF consisting at least 3 failures and 2 high quality embryo transfers were recruited. logistic regression analysis for immunological profile completed predict success compare pregnancy rates between groups ≤10.6% >10.6% phase. receiving operating curve...

Background: To identify the correlation among female age, cellular aging markers, and aneuploidy rate in vitro fertilization (IVF) preimplantation genetic test for (PGT-A) cycles. Methods: This is a prospective cohort study recruiting 110 infertile women between August 2017 July 2018. They were divided into young-age (<38 years, n = 60) advanced-age (≥38 50) groups. Peripheral leukocytes assessed, granulosa cells pooled during oocyte pickup. Mitochondrial DNA (mtDNA) copy number telomere...

Is the timing of vitrification after trophectoderm (TE) biopsy associated with successful implantation and pregnancy embryo transfer blastocysts subjected to preimplantation genetic screening (PGS)? In this retrospective cohort study, 1329 from 223 patients were TE for performing array comparative genomic hybridization (CGH) tests. The PGS frozen blastocyst (FET) cycles performed December 2012 May 2015. Only good quality expanded on day 5 or 6 selected biopsy. After biopsy, re-expansion...

This study determined the correlation between levels of reactive oxygen species (ROS) in prepared culture media and early development human embryos. was an autocontrolled comparison study. A total 159 patients undergoing vitro fertilization/intracytoplasmic sperm injection treatment were recruited this The pH values, osmolarity pressures, ROS 15 batches two measured. Sibling oocytes or embryos from individual randomly assigned to groups with Quinn's Advantage Cleavage Blastocyst (QAC/QAB)...

In vitro fertilization (IVF) treatment has gradually adopted the practice of culturing embryos until blastocyst stage on D5 or D6 as standard approach. PGT-A is commonly used in (IVF). This study aimed to evaluate clinical outcomes frozen embryo transfers (FETs) using single (SBTs) fifth (D5) sixth (D6) day development cycles that underwent preimplantation genetic testing for aneuploidy (PGT-A).The patients who had at least one euploid mosaic good quality determined by results and received...

Optimizing endometrial thickness (EMT) is crucial for successful embryo implantation, but enhancing thin endometrium remains a significant challenge. Platelet-rich plasma (PRP)-derived therapies have emerged as promising approach in reproductive medicine due to their capacity facilitate tissue repair and regeneration. This study aims identify the risk factors associated with failure of intrauterine PRP infusion women recurrent implantation (RIF). We retrospectively reviewed data from 77 RIF,...