A5102853580- Reproductive Biology and Fertility
- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- Renal and related cancers
- Pluripotent Stem Cells Research
- Reproductive Health and Technologies
- COVID-19 Impact on Reproduction
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- Metal complexes synthesis and properties
- Viral gastroenteritis research and epidemiology
- Sperm and Testicular Function
- Organometallic Complex Synthesis and Catalysis
- Metal-Catalyzed Oxygenation Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Ovarian function and disorders
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Privacy-Preserving Technologies in Data
- Magnetism in coordination complexes
- Ectopic Pregnancy Diagnosis and Management
- Intimate Partner and Family Violence
National Chung Hsing University
2002-2025
Women's Hospital
2018-2023
Taichung Hospital
2019-2023
Agricultural Technology Research Institute
2018-2019
Women’s Health Care
2018
National Changhua University of Education
1998
National Taiwan University
1998
Abstract Background Several studies have demonstrated that iDAScore is more accurate in predicting pregnancy outcomes cycles without preimplantation genetic testing for aneuploidy (PGT-A) compared to KIDScore and the Gardner criteria. However, effectiveness of with PGT-A has not been thoroughly investigated. Therefore, this study aims assess association between artificial intelligence (AI)-based (version 1.0) single-embryo transfer (SET) PGT-A. Methods This retrospective was approved by...
The porcine epidemic diarrhoea virus (PEDV) devastates the health of piglets but may not infect whose CMP-N-glycolylneuraminic acid hydroxylase (CMAH) gene is mutated (knockouts, KO) by using CRISPR/Cas9 editing techniques. This hypothesis was tested KO that were challenged with PEDV. Two single-guide RNAs targeting CMAH and Cas9 mRNA microinjected into cytoplasm newly fertilized eggs. Four live founders generated proven to be biallelic KO, lacking detectable N-glycolylneuraminic (NGNA)....
Background: To identify the correlation among female age, cellular aging markers, and aneuploidy rate in vitro fertilization (IVF) preimplantation genetic test for (PGT-A) cycles. Methods: This is a prospective cohort study recruiting 110 infertile women between August 2017 July 2018. They were divided into young-age (<38 years, n = 60) advanced-age (≥38 50) groups. Peripheral leukocytes assessed, granulosa cells pooled during oocyte pickup. Mitochondrial DNA (mtDNA) copy number telomere...
The reaction of bis(2-pyridyl) disulfide with [HFe(CO)4]- affords [Fe(CO)4(SC5H4N)]-, in which the pyridine-2-thiolate ligand is bound a monodentate (S-bonded) mode. Oxidative addition to [Fe(CO)4(SC5H4N)]- and concomitant displacement [SC5H4N]- leads neutral monomeric compound cis-[Fe(CO)2(SC5H4N)2]. In presence CH3CN at 60 °C, cis-[Fe(CO)2(SC5H4N)2] undergoes facile conversion d6 Fe(II) high-spin [Fe(SC5H4N)3]-. Reduction [Fe(SC5H4N)3]- under CO room temperature [BH4]-/[Na][benzophenone]...
Elective single-embryo transfers of euploid or low-level mosaic blastocysts were analyzed in this retrospective study to determine the correlations live birth (LB) probability with embryonic developmental features implanted day 5 (D5, n = 245) 6 (D6, 73) using time-lapse (TL) monitoring. According logistic regression analyses (adjusted odds ratio [OR] 0.341, 95% confidence interval [CI] 0.169-0.685, P < 0.05), LB was negatively associated D6 group. The rate D5 group higher than (88.2% vs....
Avoiding aneuploid embryo transfers has been shown to improve pregnancy outcomes in patients with implantation failure and loss. This retrospective cohort study aims analyze the correlation of time-lapse (TL)-based variables numeric blastocyst morphological scores (TLBMSs) different mosaic levels. In total, 918 biopsied blastocysts assessments at a uniform time-point were subjected next-generation sequencing-based preimplantation genetic testing for aneuploidy. consideration patient-...
Abstract Background For women undergoing in vitro fertilization (IVF), the clinical benefit of embryo transfer at blastocyst stage (Day 5) versus cleavage 3) remains controversial. The purpose this study is to compare implantation rate, pregnancy rate and odds live birth Day 3 5 transfer, more importantly, address issue that patients were chosen receive either protocol due their underlying characteristics, i.e., confounding by indication. Methods We conducted a retrospective cohort 9,090 IVF...
A series of linear trinuclear selenolate complexes the general type [(OC)3Fe(µ-SePh)3M(µ-SePh)3Fe(CO)3] (M = ZnII 1, CdII 2, NiII 3 or FeII 4) has been synthesized by reaction appropriate [M(H2O)n]2+ with chelating metalloligand fac-[N(PPh3)2][Fe(CO)3(SePh)3] in acetonitrile. Their structures were elucidated on basis IR, UV/VIS, NMR spectra and X-ray crystallography. Compounds 1–4 are structurally related, each containing two tridentate metalloligands resulting a MSe6 co-ordination...
This retrospective study attempts to elucidate the relevance of interval between human chorionic gonadotropin priming and oocyte pick-up (hCG-OPU) euploidy probability biopsied blastocysts in preimplantation genetic tests for aneuploidy (PGT-A) cycles. A total 1889 from 511 patients undergoing PGT- cycles were used. An analysis generalized estimating equations (GEE) was used identify whether hCG-OPU is associated with probabilities blastocysts. Accordingly, maternal age (OR: 0.925, 95% CI:...
Abstract Background For in vitro fertilization (IVF), mitochondrial DNA (mtDNA) levels the trophectodermal (TE) cells of biopsied blastocysts have been suggested to be associated with cells’ developmental potential. However, scholars reached differing opinions regarding use mtDNA as a reliable biomarker for predicting IVF outcomes. Therefore, this study aims assess association copy number measured by mitoscore embryonic characteristics and ploidy. Methods This retrospective analyzed embryos...
Experiments were conducted to evaluate the effect of blastomere diameters and cell cycle stages on subsequent development nuclear transplant rabbit embryos (NT-embryos) using nuclei derived from 16-or 32-cell stage embryos.All blastomeres NT-embryos cultured individually in modified Ham's F-10 medium supplemented with 10% serum (RS) at 38°C 5% CO 2 air.The diameter 16-cell was found twice those (51 vs 27 µm).Significant differences observed cleavage rates (≥3 divisions) isolated single (54...
This study evaluated whether the concentration of biphasic O2 (5-2%) promotes formation qualified blastocysts (QBs) and euploid probability cycles with transferable blastocysts. The paired experimental design included a total 90 patients (180 cycles) without in previous monophasic (5%) were enrolled for an additional cycle (5-2%). In group, QB rate (35.8%, 225/628) was significantly higher than that group (23.5%, 137/582; p < 0.001). addition, blastocyst number (0.5 ± 0.8) percentage (57.8%,...
Abstract Background: Following preimplantation genetic testing for aneuploidy (PGT-A) cycles, the postimplantation development of vitrified-warmed blastocysts expanded on day 5 (D5) versus (vs.) 6 (D6) has remained unclear, indicating that ploidy status may not be only intrinsic factor influencing implanted embryos. Methods: Single-embryo transfers (SETs) euploid or low-level mosaic were performed between January 2018 and December 2020. Continuous TL monitoring was then conducted to evaluate...
Abstract This study evaluated whether the concentration of biphasic O 2 (5–2%) promotes formation qualified blastocysts (QBs) and euploid increases probability cycles with transferable blastocysts. Total 90 patients (180 cycles) without in previous monophasic (5%) were enrolled for an additional cycle (5–2%). In tension group, rate QB (35.8%, 225/628) significantly higher than those group ( 23.5%, 137/582; p < 0.001). addition, number (0.5 ± 0.8) percentage (57.8%, 52/90) (0 35.6%, 32/90,...