A5046245004- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Carbon and Quantum Dots Applications
- Advanced Nanomaterials in Catalysis
- Silymarin and Mushroom Poisoning
- Insect and Pesticide Research
- Trace Elements in Health
- Mercury impact and mitigation studies
- Molecular Sensors and Ion Detection
- Graphene and Nanomaterials Applications
- Nanocluster Synthesis and Applications
- Arsenic contamination and mitigation
- Mycotoxins in Agriculture and Food
- CRISPR and Genetic Engineering
- Identification and Quantification in Food
- Antimicrobial Resistance in Staphylococcus
- Protist diversity and phylogeny
- Plant and Fungal Interactions Research
- Infective Endocarditis Diagnosis and Management
- Advanced Biosensing Techniques and Applications
- Biochemical and biochemical processes
- Electrochemical sensors and biosensors
- Salmonella and Campylobacter epidemiology
- Reproductive tract infections research
- Curcumin's Biomedical Applications
Kunming University of Science and Technology
2018-2025
Second People's Hospital of Yunnan Province
2021
Nanozyme-mediated antioxidative therapy is a promising star for treating myriad of important diseases through eliminating excessive reactive oxygen species (ROS) such as O2·- and H2O2, critical mechanism inflammatory bowel disease (IBD). This work provides high biocompatibility iodine-copper-zinc covalent doped carbon dots (Cu,Zn,I-CDs) with the catalase (CAT)-, superoxide dismutase (SOD)- glutathione peroxidase (GPx)-like catalytic activities ulcerative colitis (UC) by scavenging...
Bacterial infection, tissue hypoxia, and inflammatory oxidative stress are several key problems in wound healing of chronic infections. Herein, a multi-enzyme-like activity exhibiting multifunctional hydrogel made up mussel-inspired carbon dot reduced-Ag (CDs/AgNPs) Cu/Fe-nitrogen-doped (Cu,Fe-NC) was designed. Due to the loss glutathione (GSH) oxidase (OXD)-like nanozyme (decomposes O2 generate superoxide anion radical (O2˙-) hydroxyl production (˙OH)), exhibited excellent antibacterial...
Amanita poisoning is one of the most deadly types mushroom poisoning. α-Amanitin main lethal toxin in amanita, and human-lethal dose about 0.1 mg/kg. Most commonly used detection techniques for α-amanitin require expensive instruments. In this study, aptamer was selected as research object, stem-loop structure original not damaged by truncating redundant bases, order to improve affinity specificity aptamer. The truncated aptamers were determined using isothermal titration calorimetry (ITC)...
A sandwich lateral flow strip assay (LFSA) using a couple of aptamers functionalized with gold nanoparticles (AuNPs) was designed to assess the presence rongalite in agrifood products. More specifically, biotin-labeled primary A09 aptamer immobilized on streptavidin-coated membrane and secondary B09 conjugated AuNPs were developed as capturing signaling probes, respectively. This system allows successful direct detection food samples concentrations low 1 μg/mL, simply by observing color...
Escherichia coli, Staphylococcus aureus, Shigella, Pseudomonas aeruginosa, and Klebsiella pneumoniae are common foodborne pathogens. In this study, the light-induced PMAxx-coupled multiplex PCR (PMAxx-mPCR) was established to detect aforementioned five pathogens in fresh juice at same time. Moreover, PMAxx pretreatment could effectively distinguish live bacteria from dead bacteria. The optimized conditions were incubation with a final concentration of 10 μmol/L for min then photolysis 8 min....
Fumonisin FB is produced by Fusarium moniliforme Sheld, of which FB1 the most common and toxic. The establishment a rapid detection method an important means to prevent control pollution. A highly sensitive fluorescent sensor based on aptamer for fumonisin B1 (FB1) in corn was established. In this study, 5-carboxyfluorescein (FAM) labeled (F10). F10 adsorbed surface graphene oxide (GO) π-π stacking. FAM fluorescence signal could be quenched resonance energy transfer between molecules (GO)....
Accidental ingestion of poisonous mushrooms leading to poisoning is a global issue. The most important and lethal toxin causing mushroom α-amanitin, with dose about 0.1 mg kg-1. Rapid detection wild before consumption or rapid identification toxins after can effectively reduce the occurrence fatalities. This study established method for detecting α-amanitin using carbon dots/AuNPs nanoenzymes (D-Glu-CDs/AuNPs) robust peroxidase-like activity. nanoenzyme was prepared employing glucose dots...
Salmonella, a common foodborne pathogen, causes many cases of illness and poses threat to public health worldwide. Immunological detection systems can be combined with nanoparticles develop sensitive portable technologies for timely screening Salmonella infections. Here, we developed an antibody-probe-based immuno-N-hydroxysuccinimide (NHS) bead (AIB) system detect Salmonella. After adding the antibody probe, accumulated in samples on surfaces immuno-NHS beads (INBs), forming sandwich...
Sudan dye is generally used as a coloring agent in textile and cosmetic industries. However, exhibits potentials to cause cancer, toxicity, allergy because of its metabolites including amines naphthol related substances. Despite banning dye, the by many unscrupulous food manufacturers for enhancing appearance. This paper introduces new aptamer based on III detection methods. An vitro selection amplification method named SELEX (Systematic Evolution Ligands Exponential Enrichment) employed...
Because of diverse adverse effects carbendazim (CBZ) upon the human body and environment, a simple method for determination this molecule is urgently needed. Therefore, based on an aptamer was developed. An carbendazim, CZ12, labeled with fluorescent (FAM), adsorbed graphene oxide (GO) by π-π stacking. The fluorescence resonance energy transfer (FRET) between GO used to rapidly quench signal. In presence selectively combines thereby greatly reducing binding efficiency former GO. Thus, mixing...
Deoxynivalenol (DON) is a mycotoxin commonly found in cereals and animal feeds, which also carcinogen easily soluble water. So it has drawn considerable interest food safety. In this study, specific aptamer named DON-A16 was developed from an immobilized ssDNA library by affinity chromatography column-based the SELEX. The complementary chain competition method for DON detection established, its limit of (LOD) 488 ng/mL. Graphene oxide-based fluorescence quenching using with fluorescein (FAM)...
Fumonisin B1 (FB1) is a strong mycotoxin that ubiquitous in agricultural products. The establishment of rapid detection methods an important means to prevent and control FB1 contamination. In this study, improved enzyme-linked oligonucleotide assay (ELONA) method was designed tested detect the contents maize (corn) samples. F10 modified with biotin bound enzyme label plate coated streptavidin (SA) advance, carbon dots (CDs) were used catalyze color tetramethylbenzidine (TMB). complementary...
develop aptamer-regulated CDs for detection of FB 1 by catalytic fluorescence. 2,2′-Dithiosalicylic acid was used to prepare carbon dots catalysing the fluorescence TMB. Aptamer F10 can bind , restoring capacity and increasing
Abstract New drugs for the treatment of bacterial vaginosis (BV) are yet to be developed due concerns that they may contribute increase in antibiotic resistance BV. Antimicrobial peptides (AMPs) one most promising options next-generation antibiotics. In this study, we investigated bacteriostatic activity AMPs Pexiganan, plectasin, melittin, and cathelicidin-DM against Gram-negative Gram-positive bacteria both vitro a mouse model BV infection. The results showed had significant antibacterial...
Tuberculosis is a highly infectious bacterial disease caused by Mycobacterium tuberculosis .