A5061708061- Mosquito-borne diseases and control
- HIV Research and Treatment
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Studies on Chitinases and Chitosanases
- Advanced Nanomaterials in Catalysis
- Microfinance and Financial Inclusion
- Metal-Organic Frameworks: Synthesis and Applications
- Viral gastroenteritis research and epidemiology
- Multicomponent Synthesis of Heterocycles
- Biochemical and Molecular Research
University of Castilla-La Mancha
2021-2025
Hospital General Universitario de Albacete
2025
Translation errors, impaired folding or environmental stressors (e.g. infection) can all lead to an increase in the presence of misfolded proteins. These activate cellular responses their removal, including intracellular protein degradation activities. Protein ubiquitylation is involved two major pathways, ubiquitin-proteasome system and selective autophagy. In humans, ubiquitin-like modifier-activating enzyme 1 (UBA1) primary E1 ubiquitin conjugation cascade. Viruses have evolved exploit...
Arthropod-borne flaviviruses, such as Zika virus (ZIKV), Usutu (USUV), and West Nile (WNV), are a growing cause of human illness death around the world. Presently, no licensed antivirals to control them available and, therefore, search for broad-spectrum antivirals, including host-directed compounds, is essential. The PI3K/Akt pathway controls essential cellular functions involved in cell metabolism proliferation. Moreover, Akt has been found participate modulating replication different...
Metal-organic frameworks (MOFs) are highly versatile materials. Here, two novel MOFs, branded as IEF-23 and IEF-24 based on an antibacterial tricarboxylate linker zinc or copper cations, holding properties, presented. The materials were synthesized by the solvothermal route fully characterized. activity of was investigated against Staphylococcus epidermidis Escherichia coli via agar diffusion method. These bacteria some most broadly propagated pathogens more prone to development resistance....
In the course of experiments aimed at deciphering inhibition mechanism mycophenolic acid and ribavirin in hepatitis C virus (HCV) infection, we observed an inhibitory effect nucleoside guanosine (Gua). Here, report that Gua, not other standard nucleosides, inhibits HCV replication human hepatoma cells. Gua did directly inhibit vitro polymerase activity NS5B, but it modified intracellular levels di- tri-phosphates (NDPs NTPs), leading to deficient RNA reduction infectious progeny production....
Usutu virus (USUV) is a flavivirus that mainly infects wild birds through the bite of Culex mosquitoes. Recent outbreaks have been associated with an increased number cases in humans. Despite being growing source public health concerns, there yet insufficient data on or host cell targets for infection control. In this work we investigated whether cellular kinase Akt and USUV polymerase NS5 interact co-localize cell. To aim, performed co-immunoprecipitation (Co-IP) assays, followed by...
Usutu virus (USUV) has become increasingly relevant in recent years, with large outbreaks that sporadically have affected humans being reported wildlife. Similarly to the rest of flaviviruses, USUV contains a positive-sense single-stranded RNA genome which is replicated by activity nonstructural protein 5 (NS5). NS5 shows high sequence identity remaining viruses this genus. This permitted us identify predicted methyltransferase domain and RNA-dependent polymerase (RdRpD). Owing their degree...
Hepatitis C virus (HCV) is a single-stranded RNA of positive polarity [ssRNA(+)] that replicates its genome through the activity one proteins, called NS5B. This viral protein responsible for copying positive-polarity into negative-polarity strand, which will be template new genomes. The NS5B phosphorylated by cellular kinases, including Akt. In this work, we have identified several amino acids are Akt, with positions S27, T53, T267, and S282 giving most robust results. Site-directed...
Usutu virus (USUV) is a mosquito-borne arbovirus that has rapidly propagated in birds across several European countries over the last two decades, leading to substantial avian mortalities. USUV infection humans been associated with growing number of cases neurological disease years, underlining need for increased awareness and suitable treatments. Our group working on characterization NS5 protein USUV. This responsible replication activity viral genome can be target treat infection. contains...