A5000965333- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Hepatitis C virus research
- HIV/AIDS Research and Interventions
- Hepatitis B Virus Studies
- Historical Studies in Science
- Historical Studies of Medieval Iberia
- Medicine and Dermatology Studies History
- Biochemical and Molecular Research
- Evolution and Genetic Dynamics
- Cytomegalovirus and herpesvirus research
- Historical Studies on Spain
- Immunodeficiency and Autoimmune Disorders
- Archaeological and Historical Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Mosquito-borne diseases and control
- Medieval Iberian Studies
- HIV-related health complications and treatments
- Historical Art and Architecture Studies
- Hepatitis Viruses Studies and Epidemiology
- Liver Disease Diagnosis and Treatment
- Medieval and Classical Philosophy
- Medieval and Early Modern Iberia
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
University of Castilla-La Mancha
2014-2025
Consejo Superior de Investigaciones Científicas
1994-2023
Museo Nacional de Ciencias Naturales
2021-2023
Hospital General Universitario de Albacete
2015-2022
Pompeu Fabra University
2004-2012
Centro Nacional de Biopreparados (Cuba)
2008
Hospital Clínico San Carlos
2007
Centro de Biología Molecular Severo Ochoa
1999-2004
Universidad Autónoma de Madrid
1999-2004
Instituto de Salud Carlos III
1996-2000
HCV affects 185 million people worldwide and leads to death morbidities. has a high genetic diversity is classified into seven genotypes 67 subtypes. Novel anti-HCV drugs (Direct-Acting-Antivirals) eligibility, resistance cure rates depend on geno/subtype (GT).Analysis of epidemiological information viral GT from patients undergoing genotyping in 2011-2015.Anonymized 52 centers was analyzed retrospectively.37,839 samples were included the study. We show that distribution similar throughout...
Despite the efficacy and safety of SARS-CoV-2 vaccines, inflammatory and/or thrombotic episodes have been reported. Since impact COVID-19 vaccines on endothelium remains uncertain, our objective was to assess endothelial activation status before 90 days after third dose BNT162b2 mRNA vaccine. A prospective longitudinal study conducted at University General Hospital Albacete, involving 38 healthy health-care workers. Serum levels markers (endocan sVCAM-1) spike S1-specific IgG antibodies were...
// Jesús García-Cano 1 , Gorbatchev Ambroise 2, * Raquel Pascual-Serra 1, Maria Carmen Carrión 3, 4 Leticia Serrano-Oviedo Marta Ortega-Muelas Francisco J. Cimas Sebastià Sabater 5 María José Ruiz-Hidalgo 6, 7 Isabel Sanchez Perez 8, Antonio Mas 7, 9 Félix A. Jalón 3 Aimé Vazquez 2 Ricardo Sánchez-Prieto 4, Unidad de Medicina Molecular, Centro Regional Investigaciones Biomédicas. Universidad Castilla-La Mancha,...
ABSTRACT Nucleotide sequences of the reverse transcriptase (RT) coding region have been compared in four new human immunodeficiency virus type 1 (HIV-1) group O isolates. Phylogenetic analysis this pol highlights a cluster these HIV-1 with seven other isolates (5% intracluster nucleotide sequence diversity) similar to clusters classified as subtypes M (an average 4.9% intrasubtype diversity). Based on analyses, has designated subtype A-O. A longitudinal study heterosexual couple infected...
ABSTRACT The genomes of positive-strand RNA [(+)RNA] viruses perform two mutually exclusive functions: they act as mRNAs for the translation viral proteins and templates replication. A universal key step in replication (+)RNA is coordinated transition genome from cellular machinery to complex. While host factors are involved this step, their nature largely unknown. By using ability higher eukaryotic virus brome mosaic (BMV) replicate yeast, we previously showed that Lsm1p protein required...
LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how act on their targets remains elusive. Here, we report that reconstituted recombinant directly bind two distinct RNA-target sequences BMV genome, a tRNA-like structure at 3'-untranslated region internal A-rich single-stranded regions. Importantly, vivo analysis shows these regulate genome. Furthermore, both resemble those...
Abstract Motivation Trait variation within species can reveal plastic and/or genetic responses to environmental gradients, and may indicate where local adaptation has occurred. Here, we present a dataset of rangewide in leaf traits from seven the most ecologically economically important tree Europe. Sample collection trait assessment are embedded GenTree project (EU‐Horizon 2020), which aims at characterizing phenotypic variability forest optimize management sustainable use resources. Our...
Recent studies have shown that the accumulation of multiple mutations associated with nucleoside reverse transcriptase inhibitor (NRTI) resistance may be grouped as multi-NRTI (MNR) complexes. In this study, we examined viral fitness recombinant viruses carrying (RT) a human immunodeficiency virus type 1 (HIV-1) primary isolate harboring comprising MNR 69 insertion complex. Different RT mutants were prepared in sequence context either wild-type HIV-1(BH10) or found clinical HIV-1 mutation....
New mutations provide the raw material for evolution and adaptation. The distribution of fitness effects (DFE) describes spectrum new that can occur along a genome, is, therefore, vital interest in evolutionary biology. Recent work has uncovered striking similarities DFE between closely related species, prompting us to ask whether there is variation among populations same or species with different degrees divergence, at levels evolution. Using exome capture data from six tree sampled across...
Hepatitis C virus (HCV) shows a great geographical diversity reflected in the high number of circulating genotypes and subtypes. The response to HCV treatment is genotype specific, with predominant 1 showing lowest rate sustained virological response. Virally encoded enzymes are candidate targets for intervention. In particular, promising antiviral molecules being developed target viral NS3/4A protease NS5B polymerase. Most studies polymerase have been done 1b 2a, whilst information about...
Arthropod-borne flaviviruses, such as Zika virus (ZIKV), Usutu (USUV), and West Nile (WNV), are a growing cause of human illness death around the world. Presently, no licensed antivirals to control them available and, therefore, search for broad-spectrum antivirals, including host-directed compounds, is essential. The PI3K/Akt pathway controls essential cellular functions involved in cell metabolism proliferation. Moreover, Akt has been found participate modulating replication different...
Progress in the field of evolutionary forest ecology has been hampered by huge challenge phenotyping trees across their ranges natural environments, and limitation high-resolution environmental information. The GenTree Platform contains phenotypic data from 4,959 12 ecologically economically important European tree species: Abies alba Mill. (silver fir), Betula pendula Roth. birch), Fagus sylvatica L. (European beech), Picea abies (L.) H. Karst (Norway spruce), Pinus cembra (Swiss stone...
Human immunodeficiency virus (HIV)-1 strains have been divided into three groups: main (M), outlier (O), and non-M non-O (N). Biochemical analyses of HIV-1 reverse transcriptase (RT) performed predominantly with enzymes derived from group M:subtype B laboratory strains. This study was designed to optimize the expression characterize enzymatic properties O RTs as well chimeric composed M p66 p51 subunits. The DNA-dependent DNA polymerase activity on a short heteropolymeric template-primer...
A deletion of 32 base pairs in the CCR5 gene (Δ32 CCR5) has been linked to resistance HIV-1 infection exposed adults and delay disease progression infected adults. To determine role Δ32 children born seropositive mothers, we studied a polymerase chain reaction 301 infected, 262 exposed-uninfected 47 unexposed-uninfected Spanish Italian origin. Infected were further divided into two groups according their rate progression: rapid progressors who developed severe clinical and/or immunological...