A5089634270- Brucella: diagnosis, epidemiology, treatment
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Galectins and Cancer Biology
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Parasitic infections in humans and animals
- Bacillus and Francisella bacterial research
- Immune cells in cancer
- Bacterial Identification and Susceptibility Testing
- Long-Term Effects of COVID-19
- Yersinia bacterium, plague, ectoparasites research
- Congenital Anomalies and Fetal Surgery
- Sphingolipid Metabolism and Signaling
- Bacterial biofilms and quorum sensing
- COVID-19 and healthcare impacts
- Hepatitis Viruses Studies and Epidemiology
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Legionella and Acanthamoeba research
- HIV, Drug Use, Sexual Risk
- Burkholderia infections and melioidosis
Hospital Clínico Universitario de Valladolid
2010-2024
Universidad de Valladolid
2005-2023
Departamento de Salud
2016
Instituto Tecnológico Agrario de Castilla y León
2014
Umeå University
2014
Complejo Asistencial Universitario de Palencia
2014
Universidad de León
2014
Universidad de Burgos
2014
Universidad de Salamanca
2005-2007
Instituto de Recursos Naturales y Agrobiología de Salamanca
2007
Abstract Background COVID-19 can course with respiratory and extrapulmonary disease. SARS-CoV-2 RNA is detected in samples but also blood, stool urine. Severe characterized by a dysregulated host response to this virus. We studied whether viral RNAemia or load plasma associated severe response. Methods A total of 250 patients were recruited (50 outpatients, 100 hospitalized ward critically ill). Viral detection quantification was performed using droplet digital PCR, targeting the N1 N2...
Abstract SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is the strain causing pandemic COVID-19 (coronavirus disease 2019). To understand pathobiology of in humans it necessary to unravel metabolic changes that are produced individuals once infection has taken place. The goal this work provide new information about altered biomolecule profile and with biological pathways patients different clinical situations due infection. This done via metabolomics using HPLC–QTOF–MS analysis...
ABSTRACT We evaluated the validity and usefulness of a new test for diagnosis human brucellosis based on an immunocapture-agglutination technique. A total 315 sera from 82 patients with brucellosis, 157 in whom was suspected but not confirmed, 412 people living rural areas endemic were studied. The seroagglutination (SAT), Coombs anti-Brucella test, Brucellacapt evaluated. All initial proved to be positive tests, while only 75 (91.4%) SAT. If ≥1/160 diagnostic threshold titer defined SAT,...
HCV affects 185 million people worldwide and leads to death morbidities. has a high genetic diversity is classified into seven genotypes 67 subtypes. Novel anti-HCV drugs (Direct-Acting-Antivirals) eligibility, resistance cure rates depend on geno/subtype (GT).Analysis of epidemiological information viral GT from patients undergoing genotyping in 2011-2015.Anonymized 52 centers was analyzed retrospectively.37,839 samples were included the study. We show that distribution similar throughout...
The SARS-CoV-2 infection has widely spread to become the greatest public health challenge date, COVID-19 pandemic. Different fatality rates among countries are probably due non-standardized records being carried out by local authorities. Spanish case-fatality rate is 11.22%, far higher than those reported in Asia or other European countries. A multicentre retrospective study of demographic, clinical, laboratory and immunological features 584 hospitalized patients their outcomes was...
The functions of macrophages are tightly regulated by their metabolic state. However, the role mitochondrial electron transport chain (ETC) in macrophage remains understudied. Here, we provide evidence that succinate dehydrogenase (SDH)/complex II (CII) is required for respiration and plays a controlling effector responses macrophages. We find absence catalytic subunits Sdha Sdhb impairs ability to effectively stabilize HIF-1α produce pro-inflammatory cytokine IL-1β response LPS stimulation....
A standardized test for the serodiagnosis of human cystic echinococcosis (CE) is still needed, because low specificity and sensitivity currently available commercial tools lack proper evaluation existing recombinant antigens. In a previous work, we defined new ELISA-B2t diagnostic tool detection specific IgGs in CE patients, which showed high specificity, was useful monitoring clinical evolution surgically treated patients. Nevertheless, this antigen gave rise to false-negative results...
ABSTRACT Four different recombinant antigens derived from Echinococcus granulosus , designated B1t, B2t, E14t, and C317, were tested with enzyme-linked immunosorbent assays (ELISAs) for the detection of specific immunoglobulin G (IgG) in patients unilocular hydatid disease (UHD). The results compared to those obtained fluid subjected receiver operator characteristic analysis. diagnostic performance above-listed proteins was defined respect their specificity, sensitivity, predictive values...
Francisella tularensis is a virulent Gram-negative intracellular pathogen. To address the signaling routes involved in response of host cells to LPS from F. live vaccine strain (LVS), experiments were performed transiently transfected 293 cells. Induction κB-driven transcriptional activity by 2.5 μg ml−1F. isolated phenol–water and ether–water extraction, was observed with Toll-like receptor (TLR) 4 MD-2, although CD14 required for optimal induction. Conversely, TLR2, TLR2/TLR1 or TLR2/TLR6...
Tularemia outbreaks occurred in northwestern Spain 1997-1998 and 2007-2008 affected >1,000 persons. We assessed isolates involved these by using pulsed-field gel electrophoresis with 2 restriction enzymes multilocus variable number tandem repeat analysis of 16 genomic loci Francisella tularensis, the cause this disease. Isolates were divided into 3 pulsotypes 8 allelic profiles analysis. obtained from second tularemia outbreak had same genotypes as first outbreak. Both caused genetic...
Colon cancer is a devastating illness that associated with gut inflammation. Here, we explored the possible role of lipin-1, phosphatidic acid phosphatase, in development colitis-associated tumorigenesis. Azoxymethane and dextran sodium sulfate-treated (DSS-treated) animals deficient lipin-1 harbored fewer tumors carcinomas than WT due to decreased cellular proliferation, lower expression antiapoptotic protumorigenic factors, reduced infiltration macrophages colon tumors. They also displayed...
The Fas-activated serine/threonine kinase (FASTK) family of proteins has recently emerged as a central regulator mitochondrial gene expression through the function an unusual RNA-binding domain named RAP (for abundant in Apicomplexans), shared by all six members family. Here we describe role one less characterized members, FASTKD3, RNA metabolism. First, show that, contrast to FASTK, FASTKD2, and FASTKD5, FASTKD3 does not localize granules, which are sites processing maturation mtRNAs...
Genetic predisposition to coronavirus disease 2019 (COVID-19) may contribute its morbidity and mortality. Because cytokines play an important role in multiple phases of infection, we examined whether commonly occurring, functional polymorphisms macrophage migration inhibitory factor (MIF) are associated with COVID-19 infection or severity.To determine associations common MIF symptomatic severity.This retrospective case-control study utilized 1171 patients from three tertiary medical centers...
Vascular inflammation is a major atherogenic factor and Toll-like receptor (TLR) 2 ligands, including bacterial serum lipoproteins, seem to be involved in atherogenesis. On this basis, we analysed the effect of lipoproteins different lipid components on TLR2-dependent signalling.In TLR2-transfected human embryonic kidney 293 cells monocytes, oxidized low-density inhibited nuclear (NF)-kappaB-driven transcriptional activity chemokine gene expression response TLR2 ligands. Sphingosine...
ABSTRACT Smooth lipopolysaccharide (S-LPS) and lipid A of Brucella abortus melitensis induced the production nitric oxide (NO) by rat adherent peritoneal cells, but they lower levels NO than Escherichia coli LPS. The participation inducible isoform synthase (iNOS) was confirmed finding an increased expression both iNOS mRNA protein. These observations might help to explain (i) acute outcome infection in rodents, (ii) low frequency septic shock human brucellosis, (iii) prolonged intracellular...
Abstract We generated Fas-activated serine threonine phosphoprotein (FAST)-deficient mice (FAST−/−) to study the in vivo role of FAST immune system function. In a model house dust mite-induced allergic pulmonary inflammation, wild type develop mixed cellular infiltrate composed eosinophils, lymphocytes, and neutrophils. FAST−/− airway inflammation that is distinguished by near absence Similarly, LPS-induced alveolar neutrophil recruitment markedly reduced compared with controls. This...