A5001599348- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Immune responses and vaccinations
- COVID-19 Clinical Research Studies
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Cell death mechanisms and regulation
- Macrophage Migration Inhibitory Factor
- Education and Character Development
- Immune Response and Inflammation
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
Instituto de Biomedicina y Genética Molecular de Valladolid
2022-2024
Universidad de Valladolid
2022-2024
Instituto de Salud Carlos III
2022
Centro de Investigación Biomédica en Red
2022
Centre d’Immunologie de Marseille-Luminy
2016-2017
Inserm
2016-2017
Aix-Marseille Université
2016-2017
Centre National de la Recherche Scientifique
2016-2017
Universidad de Cádiz
2010-2012
Hospital Universitario Puerto Real
2012
T-cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by E3 ubiquitin-protein ligases CBL CBLB Here, we combined mouse genetics affinity purification coupled to quantitative mass spectrometry monitor dynamics complexes that assemble in normal over 600 seconds TCR stimulation. We identify most previously known interacting partners, as well a majority proteins have not yet been implicated those complexes. exploit correlations protein association with...
Genetic predisposition to coronavirus disease 2019 (COVID-19) may contribute its morbidity and mortality. Because cytokines play an important role in multiple phases of infection, we examined whether commonly occurring, functional polymorphisms macrophage migration inhibitory factor (MIF) are associated with COVID-19 infection or severity.To determine associations common MIF symptomatic severity.This retrospective case-control study utilized 1171 patients from three tertiary medical centers...
Highlights•Temporal proteomic profiling in primary cells using DIA/SWATH mass spectrometry•Identification of GRB2 interactors T cells•Developing and mature show distinct dynamics protein interactions•Broad applicability our workflow for dissecting mammalian signaling systemsSummarySpatiotemporal organization interactions cell is a fundamental process that drives cellular functions. Given differential expression across tissues developmental stages, the architecture interaction proteomes is,...
ABSTRACT The adaptor protein LAT has a prominent role in the transduction of intracellular signals elicited by TCR/CD3 complex. Upon TCR engagement, becomes tyrosine-phosphorylated and thereby, recruits to membrane several proteins implicated activation downstream signaling pathways. However, little is known about other conserved motifs present sequence. Here, we report that contains serine-based motifs, which are essential for proper signal through TCR. Mutation these serine human T cell...
Engagement of the TCR (T-cell receptor) induces tyrosine phosphorylation LAT (linker for activation T-cells) adaptor, and thereby it recruits several cytosolic mediators downstream signalling pathways. The Fas protein is essential T-lymphocyte apoptosis, following engagement, many proteins are proteolytically cleaved, including molecules that important transduction intracellular signals. In present study, we demonstrate adaptor also subject to a proteolytic cleavage in mature T-lymphocytes...
Introduction Coronavirus disease 2019 (COVID-19) affects the population unequally, with a greater impact on older and immunosuppressed people. Methods Hence, we performed prospective experimental cohort study to characterise effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination in immune-compromised patients (older adults oncohaematologic patients), compared healthy counterparts, based deep characterisation circulating immune cell subsets. Results discussion...
ABSTRACT COVID-19 affects the population unequally with a higher impact on aged and immunosuppressed people. Hence, we assessed effect of SARS-CoV-2 vaccination in immune compromised patients (older adults oncohematologic patients), compared healthy counterparts. While acquired humoral cellular memory did not predict subsequent infection 18 months after full immunization, spectral computational cytometry revealed several subsets within CD8 + T-cells, B-cells, NK cells, monocytes CD45RA CCR7...