A5043468037- vaccines and immunoinformatics approaches
- Advanced Biosensing Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Metabolomics and Mass Spectrometry Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Peptidase Inhibition and Analysis
- Computational Drug Discovery Methods
- Mass Spectrometry Techniques and Applications
- Child Abuse and Related Trauma
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- Neuroscience of respiration and sleep
- RNA modifications and cancer
- S100 Proteins and Annexins
- Click Chemistry and Applications
- Antimicrobial Peptides and Activities
- Endoplasmic Reticulum Stress and Disease
- Metabolism, Diabetes, and Cancer
- Neonatal Respiratory Health Research
- Restraint-Related Deaths
- Traumatic Brain Injury and Neurovascular Disturbances
University Centre of Legal Medicine
2022-2025
University of Lausanne
2009-2025
University Hospital of Geneva
2022-2024
ETH Zurich
2014-2023
École Polytechnique Fédérale de Lausanne
2021-2023
The Geneva Association
2019
University of Geneva
2009-2015
Down syndrome (DS) is mostly caused by a trisomy of the entire Chromosome 21 (Trisomy 21, T21). Here, we use SWATH mass spectrometry to quantify protein abundance and turnover in fibroblasts from monozygotic twin pair discordant for T21, profile expression 11 unrelated DS individuals matched controls. The integration steady-state proteomic data indicates that protein-specific degradation members stoichiometric complexes major determinant T21 gene dosage outcome, both within between...
Cancer is mostly incurable when diagnosed at a metastatic stage, making its early detection via blood proteins of immense clinical interest. Proteomic changes in tumor tissue may lead to detectable the protein composition circulating plasma. Using proteomic workflow combining N-glycosite enrichment and SWATH mass spectrometry, we generate data resource 284 samples derived from patients with different types localized-stage carcinomas matched controls. We observe whether patient's plasma are...
Obesity is considered as a major public health concern with strong economic and social burdens. Exposure to pollutants such heavy metals can contribute the development of obesity its associated metabolic disorders, including type 2 diabetes cardiovascular diseases. Adipose tissue an endocrine paracrine organ that plays key role in these diseases one main target metal accumulation. In this study, we determined by inductively coupled plasma mass spectrometry cadmium concentrations human...
Abstract Purpose: Despite high clinical need, there are no biomarkers that accurately predict the response of patients with metastatic melanoma to anti-PD-1 therapy. Experimental Design: In this multicenter study, we applied protein depletion and enrichment methods prior various proteomic techniques analyze a serum discovery cohort (n = 56) three independent validation cohorts 80, n 12, 17). Further analyses by literature survival analysis followed. Results: We identified several...
The paralogs CUL4A and CUL4B assemble cullin-RING E3 ubiquitin ligase (CRL) complexes regulating multiple chromatin-associated cellular functions. Although they are structurally similar, we found that the unique N-terminal extension of is heavily phosphorylated during mitosis, phosphorylation pattern perturbed in CUL4B-P50L mutation causing X-linked intellectual disability (XLID). Phenotypic characterization mutational analysis revealed required for efficient progression through controlling...
Highlights•Temporal proteomic profiling in primary cells using DIA/SWATH mass spectrometry•Identification of GRB2 interactors T cells•Developing and mature show distinct dynamics protein interactions•Broad applicability our workflow for dissecting mammalian signaling systemsSummarySpatiotemporal organization interactions cell is a fundamental process that drives cellular functions. Given differential expression across tissues developmental stages, the architecture interaction proteomes is,...
Abstract Microsample analysis is highly beneficial in blood-based testing where cutting-edge bioanalytical technologies enable the of volumes down to a few tens microliters. Despite availability analytical methods, difficulty obtaining high-quality and standardized microsamples at point collection remains major limitation process. Here, we detail model blood separation principle which exploits discrete viscosity differences caused by particle sedimentation laminar flow. Based on this...
Abstract MicroRNA (miRNA) loaded Argonaute (AGO) complexes regulate gene expression via direct base pairing with their mRNA targets. Previous works suggest that up to 60% of mammalian transcripts might be subject miRNA‐mediated regulation, but it remains largely unknown which fraction these interactions are functional in a specific cellular context. Here, we integrate transcriptome data from set miRNA‐depleted mouse embryonic stem cell (mESC) lines published miRNA interaction predictions and...
ABSTRACT Purpose Traumatic brain injury (TBI), including pediatric abusive head trauma (AHT), is the leading cause of death and disability in children young adults worldwide. The current understanding trauma‐induced molecular changes human subjects with intracranial hemorrhage (ICH) remains inadequate requires further investigation to improve outcome management TBI clinic. Calcium‐mediated damage at site has been shown activate several catalytic enzymes. Experimental Design Serine hydrolases...
STAT6 interacts with PPARγ to elicit macrophage polarization towards an anti-inflammatory, insulin-sensitizing phenotype. Mice deficient in display liver lipid accumulation (hepatosteatosis). Rosiglitazone (RSG), a agonist, ameliorates hepatosteatosis and enhances insulin sensitivity. To elucidate the role of action on we compared proteomes RSG-treated wild type STAT6-deficient mice identified pyruvate kinase M2 (PKM2), glycolysis proliferation-regulating enzyme that displayed...
Increased inflammatory signaling is a key feature of metabolic disorders. In this context, the role increased pro-inflammatory signals has been extensively studied. By contrast, no efforts have dedicated to study contrasting scenario: attenuation anti-inflammatory and their in homeostasis. IL-4 IL-13 are cytokines through Signal Transducer Activator Transcription 6 (STAT6). Our was aimed at evaluating lack STAT6 on liver To end we analyzed proteome wild type knock-out mice using 2D nanoscale...
Abstract To-date, most proteomic studies aimed at discovering tissue-based cancer biomarkers have compared the quantity of selected proteins between case and control groups. However, generally function in association with other to form modules localized particular subcellular compartments specialized cell types tissues. Sub-cellular mislocalization has fact been detected as a key feature variety cells. Here, we describe strategy for tissue-biomarker detection based on mitochondrial fold...
Human lifetime exposure to arsenic through drinking water, food supply or industrial pollution leads its accumulation in many organs such as liver, kidneys, lungs pancreas but also adipose tissue. Recently, population-based studies revealed the association between and development of metabolic diseases obesity type 2 diabetes. To shed light on molecular bases association, we determined concentration that inhibited 17% cell viability investigated effects acute adipose-derived human mesenchymal...
Abstract Background In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The ability to tip the balance toward hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems be critical for healthy expansion, as opposed hypertrophic growth that is accompanied development inflammation metabolic dysfunction. However, molecular mechanisms underlying fine-tuned regulation expansion are far...
Abusive head trauma (AHT) is a leading cause of mortality and morbidity in infants. While the reported incidence close to 40 cases per 100'000 births/year, misdiagnoses are commonly observed with atypical, subacute, or chronic presentation. Currently, standard clinical evaluation inflicted intracranial hemorrhagic injury (ICH) infants urgently requires screening test able identify who need additional investigations. Blood biomarkers characteristic AHT may assist detecting these infants,...
SUMMARY MicroRNA (miRNA) loaded Argonaute (AGO) complexes regulate gene expression via direct base pairing with their mRNA targets. Current prediction approaches identified that between 20 to 60% of mammalian transcriptomes are regulated by miRNAs, but it remains largely unknown which fraction these interactions functional in a specific cellular context. Here, we integrated transcriptome data from set miRNA-depleted mouse embryonic stem cell (mESC) lines published miRNA interaction...
<p>Differentially expressed proteins (DEPs) and functional annotation of NR proteomes based on GO term classification.</p>