A5104977541- Microscopic Colitis
- Celiac Disease Research and Management
- Inflammatory Bowel Disease
- Eosinophilic Esophagitis
- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Digestive system and related health
- Gastrointestinal disorders and treatments
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Respiratory viral infections research
- Autoimmune and Inflammatory Disorders
- IL-33, ST2, and ILC Pathways
- Diverticular Disease and Complications
- Neonatal Respiratory Health Research
- Viral gastroenteritis research and epidemiology
- Whipple's Disease and Interleukins
- Microbial Metabolites in Food Biotechnology
- Pancreatitis Pathology and Treatment
- Infant Nutrition and Health
- Neonatal Health and Biochemistry
- Asthma and respiratory diseases
- Gastric Cancer Management and Outcomes
- Gut microbiota and health
Instituto de Biomedicina y Genética Molecular de Valladolid
2015-2025
Universidad de Valladolid
2016-2025
Hospital Universitario La Paz
2010-2025
Hospital La Paz Institute for Health Research
2021-2025
Universidad Autónoma de Madrid
2019-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2019
Hospital Universitario de La Princesa
2019
St Mark's Hospital
2015
London North West Healthcare NHS Trust
2015
Consejo Superior de Investigaciones Científicas
2011-2014
Intestinal alterations in IBD are triggered and maintained by an overexpression of proinflammatory cytokines. Additionally, increased immune activation has been found the adjacent intestinal areas without displaying any apparent histological alterations, however, regulatory environment is not well established. Biopsy specimens from patients with ulcerative colitis (UC) Crohn's disease (CD), both affected unaffected areas, also a group colonic biopsies healthy controls, were included our...
To investigate the specific characteristics of serum procalcitonin in children with severe infection, to identify relevant factors influencing increase, assess its prognostic value, and compare it C-reactive protein neutrophil countA prospective observational study 48 hrs follow-up a cohort cases.A pediatric intensive care unit within children's university hospital collaboration laboratory mainly involved research clinical immunology.A total 80 (median age, 3.1 yrs; range, 1 month 16 yrs)...
Concentrations of immunoglobulins (Ig) and levels isotype specific antibodies to three dietary antigens in serum, pure parotid saliva, intestinal secretions obtained by whole gut lavage from groups healthy elderly subjects (aged greater than 70 years) younger adult controls 25-50 were measured. In addition, counts lamina propria intra-epithelial lymphoid cells performed histologically normal jejunal biopsy specimens subjects. Elderly had significantly higher concentrations serum salivary IgA...
Dendritic cells ( DC s) control the type and location of immune responses. Ulcerative colitis UC ) is considered a T h2 disease mediated by IL ‐13 where up to one third patients can develop extraintestinal manifestations. Colonic biopsies from inflamed noninflamed areas were cultured in vitro their supernatants used condition human blood enriched s healthy controls. Levels culture below detection limit most cases cytokine profile suggested mixed rather than profile. ‐6 was predominant found...
Scope The human/microbiota cross‐talk is partially mediated by bacteria‐derived peptides like Serine‐Threonine peptide ( ST p), which resistant to gut proteolysis, found in the human healthy colon and induces regulatory properties on dendritic cells DC s); here we characterized ulcerative colitis UC ) patients studied effect of STp their properties. Methods results Human colonic from controls were isolated, conditioned for 24 h +/− p flow cytometry, immunohistochemistry, electron microscopy....
Abstract Background Human intestinal conventional dendritic cells (cDC) maintain the mechanisms of immune tolerance in health. However, their specific contribution to development inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn´s (CD) remains elusive Methods cDC were identified by flow cytometry human mucosal, divided into CD103- cDC2, CD103+ cDC2 cDC1. Their phenotype was determined health IBD, together with stimulatory capacity over naïve T-cells. Results cDC1...
Abstract Background Severe Acute Ulcerative Colitis (ASUC) is a serious complication of UC that requires an early response to treatment assessment and which clinical analytical evaluations are often inadequate. In this setting, intestinal ultrasound (IUS) could be helpful, given its accuracy in evaluating inflammatory activity non-invasiveness. The aim study was determine whether basal IUS findings index predict ASUC patients’ corticosteroid treatment. Methods A retrospective descriptive...
Abstract Background Neoplastic risk is increased in long-standing colitis. Dye chromoendoscopy (CE) and virtual are surveillance methods. The aim of this trial to compare methylene blue with second generation narrow band imaging (NBI) the detection IBD-associated colonic neoplasia. Methods Prospective randomized controlled (NBICromo NCT04403997) including patients clinical indication for (>8 years disease, left-sided or extensive colitis, Crohn´s disease >2 segments affected...
We examined humoral immunity in coeliac disease as expressed serum (systemic immunity), and saliva, jejunal aspirate, whole gut lavage fluid (mucosal immunity). The aims were to define features of the secretory immune response (IgA IgM concentrations antibody values gliadin other food proteins measured by enzyme linked immunosorbent assay (ELISA)) active remission, establish whether secretions obtained relatively non-invasive techniques (saliva fluid) can be used for indirect measurements...
Summary The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response CD specific. We have studied expression receptor, and upon stimulation, in non-CD possible existence a lower immunological threshold latter. Forty-two patients (20 on gluten-containing diet, GCD, 22 gluten-free GFD) 24 healthy individuals were studied. IL15Rα mRNA expression, tissue characterization, assayed duodenum. Biopsies from six GFD 10 vitro using organ...
Abstract Background and Aims Primary sclerosing cholangitis [PSC] is usually associated with inflammatory bowel disease [IBD]. An increased risk of malignancies, mainly colorectal cancer [CRC] cholangiocarcinoma [CCA], has been reported in PSC-IBD patients. Our aim was to determine the clinical characteristics management PSC IBD patients, factors malignancies. Methods patients were identified from Spanish ENEIDA registry GETECCU. Additional data collected using AEG-REDCap electronic capture...
Patients with coeliac disease have IgM antibodies and IgA anti-gliadin antibody in gut secretions, also high counts of intraepithelial lymphocytes (IEL) that express the gamma/delta T cell receptor. These features intestinal immunity may be markers latent disease. Their occurrence was examined 77 patients referred for jejunal biopsy, whom biopsy histology normal, to establish extent which these, other candidate (high total IEL count, serum (AGA), increased permeability) coexist same patient....
Profiling of immune mediators in both nasal and plasma samples is a common approach to the study pathogenesis respiratory viral infections. Nevertheless, mucosal immunity functions essentially independently from peripheral immunity. In our study, 27 were profiled parallel, nasopharyngeal aspirates (NPAs) 22 < 2 year-old children with severe syncytial virus infection involving lower tract, using multiplex assay. NPAs innocent heart murmurs used as controls. Differences mediator concentrations...
The insulin-degrading enzyme (IDE) is an evolutionarily conserved zinc-dependent metallopeptidase highly expressed in the brain, where its specific functions remain poorly understood. Besides insulin, IDE able to cleave many substrates vitro, including amyloid beta peptides, making this a candidate pathophysiological link between Alzheimer's disease (AD) and type 2 diabetes (T2D). These antecedents led us address impact of absence hippocampus olfactory bulb. A induction microgliosis was...
Respiratory syncytial virus (RSV) infection is common in young children, but only a few develop severe bronchiolitis. The relationship between bronchiolitis, asthma, and atopy has been debated for long time, the pathogenesis of wheezing remains unclear. A Th1 Th2-type lymphocyte imbalance seems to be involved asthma atopic disease. Serum interleukin-12 (IL-12), IL-10, soluble CD30 (sCD30) levels were measured by enzyme-linked immunosorbent assay (ELISA) 23 cord blood samples kept frozen...
The aims of this study were to evaluate the following: 1) prevalence hypertransaminasemia (HT) in a pediatric celiac disease (CD) and its relation with clinical parameters; 2) frequency HT as only manifestation CD; 3) evolution after gluten free diet.A total 114 consecutive CD patients studied (60% classical 40% atypical forms). Antiendomisyum antibodies anti-tissue transglutaminase determined suspicion (including unexplained chronic HT), at risk, preoperative increased ALT activity for...
Dietary gluten induces an early response in the intestine of coeliac disease patients (CD), within a few hours, and this is driven by high levels proinflammatory cytokines, including IFNgamma IL-15, as has been thoroughly shown stimulation biopsy explants. Our aim was to identify immune mediators involved long-standing inflammation untreated CD at diagnosis. mRNA protein TNFalpha, IL-12(p35), IL-12(p40), IL-18 IL-23(p19) were quantified biopsies from active patients, on gluten-free diet...