A5036904194- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Mast cells and histamine
- Neuropeptides and Animal Physiology
- Olfactory and Sensory Function Studies
- Genetic Neurodegenerative Diseases
- Neurotransmitter Receptor Influence on Behavior
- Photoreceptor and optogenetics research
- Environmental Impact and Sustainability
- Polyamine Metabolism and Applications
- Biochemical Acid Research Studies
- Pharmacological Receptor Mechanisms and Effects
- Ion channel regulation and function
- Circadian rhythm and melatonin
- Sustainability and Ecological Systems Analysis
- Agriculture Sustainability and Environmental Impact
- Pluripotent Stem Cells Research
- Innovative Teaching Methods
- Protein Kinase Regulation and GTPase Signaling
- Various Chemistry Research Topics
- Neuroinflammation and Neurodegeneration Mechanisms
- Water-Energy-Food Nexus Studies
- Cholinesterase and Neurodegenerative Diseases
- Photochromic and Fluorescence Chemistry
- Sleep and Wakefulness Research
Universitat Autònoma de Barcelona
2006-2022
Universitat de Barcelona
2012-2020
Institut de Génomique Fonctionnelle
2015-2020
Inserm
2015-2020
Centre National de la Recherche Scientifique
2015-2020
Biomedical Research Networking Center on Neurodegenerative Diseases
2012-2020
Institut de Biomedicina de la Universitat de Barcelona
2014-2020
Université de Montpellier
2017-2020
UCB Pharma (Belgium)
2018-2020
Universidad de Navarra
2012
The role of the pineal gland is to translate rhythmic cycles night and day encoded by retina into hormonal signals that are transmitted rest neuronal system in form serotonin melatonin synthesis release. Here we describe production both regulated a circadian-related heteromerization adrenergic dopamine D₄ receptors. Through α(₁B)-D₄ β₁-D₄ receptor heteromers inhibits signaling blocks induced ligands. This inhibition was not observed at hours when expressed. These data provide new perspective...
Metabotropic glutamate receptors (mGluRs) are mandatory dimers playing important roles in regulating CNS function. Although assumed to form exclusive homodimers, 16 possible heterodimeric mGluRs have been proposed but their existence native cells remains elusive. Here, we set up two assays specifically identify the pharmacological properties of rat mGlu heterodimers composed mGlu2 and 4 subunits. We used either a heterodimer-specific conformational LRET-based biosensor or system that...
Abstract Antibodies have enormous therapeutic and biotechnology potential. G protein-coupled receptors (GPCRs), the main targets in drug development, are of major interest antibody development programs. Metabotropic glutamate dimeric GPCRs that can control synaptic activity a multitude ways. Here we identify llama nanobodies specifically recognize mGlu2 receptors, among eight subtypes mGluR subunits. Among these nanobodies, DN10 13 positive allosteric modulators (PAM) on homodimeric mGlu2,...
Release of the neuropeptides corticotropin-releasing factor (CRF) and orexin-A in ventral tegmental area (VTA) play an important role stress-induced cocaine-seeking behavior. We provide evidence for pharmacologically significant interactions between CRF that depend on oligomerization 1 receptor (CRF R) orexin OX receptors (OX R). R–OX R heteromers are conduits a negative crosstalk as demonstrated transfected cells rat VTA, which they significantly modulate dendritic dopamine release. The...
The general effects of cocaine are not well understood at the molecular level. What is known that dopamine D1 receptor plays an important role. Here we show a key mechanism may be cocaine's blockade histamine H3 receptor-mediated inhibition function. This requires σ1 and occurs upon binding to σ1-D1-H3 complexes. cocaine-mediated disruption leaves uninhibited activates Gs, freely recruits β-arrestin, increases p-ERK 1/2 levels, induces cell death when over activated. Using in vitro assays...
GPCRs play critical roles in cell communication. Although can form heteromers, their role signaling remains elusive. Here we used rat metabotropic glutamate (mGlu) receptors as prototypical dimers to study the functional interaction between each subunit. mGluRs both constitutive homo- and heterodimers. Whereas mGlu2 mGlu4 couple G proteins, protein activation is mediated by heptahelical domain (HD) exclusively mGlu2-4 Such asymmetric transduction results from action of dimeric extracellular...
Dopamine receptor D1 modulates glutamatergic transmission in cortico-basal ganglia circuits and represents a major target of L-DOPA therapy Parkinson's disease. Here we show that metabotropic glutamate type 5 (mGlu5) receptors can form previously unknown heteromeric entities with distinctive functional properties. Interacting Gq proteins, cell-surface D1-mGlu5 heteromers exacerbated PLC signaling intracellular calcium release response to either or dopamine. In rodent models disease,...
G protein-coupled receptors (GPCRs) are key players in cell communication and encoded by the largest family our genome. As such, GPCRs represent main targets drug development programs. Sequence analysis revealed several classes of GPCRs: class A rhodopsin-like majority, B includes secretin-like adhesion GPCRs, F frizzled receptors, C for neurotransmitters, glutamate GABA, those sweet umami taste calcium receptors. Class far more complex than other being mandatory dimers, with each subunit...
Early Huntington's disease (HD) include over-activation of dopamine D1 receptors (D1R), producing an imbalance in dopaminergic neurotransmission and cell death. To reduce D1R over-activation, we present a strategy based on targeting complexes histamine H3 (H3R). Using HD mouse striatal model organotypic brain slices found that D1R-induced death signaling neuronal degeneration, are mitigated by H3R antagonist. We demonstrate the D1R-H3R heteromer is expressed mice at early but not late stages...
H<sub>3</sub> autoreceptors provide feedback control of neurotransmitter synthesis in histaminergic neurons, but the transduction pathways involved are poorly understood. In rat brain cortical slices, histamine can be stimulated by depolarization and inhibited agonists. We show that stimulation with 30 mM K<sup>+</sup> requires extracellular calcium entry, mostly through N-type channels, subsequent activation calcium/calmodulin-dependent protein kinase type II. vitro, this phosphorylated...
Synaptic events are important to define treatment strategies for brain disorders. In the present paper, freshly obtained rat striatal minces were incubated under different times and conditions determine dopamine biosynthesis, storage, tyrosine hydroxylase phosphorylation. Remarkably, we found that endogenous spontaneously accumulated during tissue incubation at 37 °C ex vivo while synthesis simultaneously decreased. We analyzed whether these changes in biosynthesis storage linked feedback...
Summary Aims Kv1.1 (KCNA1) channels contribute to the control of neuronal excitability and have been associated with epilepsy. can associate cytoplasmic Kvβ1 subunit resulting in rapid inactivating A‐type currents. We hypothesized that removal channel inactivation, by modulating Kv1.1/Kvβ1 interaction a small molecule, would lead decreased anticonvulsant activity. Methods applied high‐throughput screening identify ligands able modulate Kv1.1‐T1 domain/Kvβ1 protein complex. then selected...
<h3></h3> In the early stages of Huntington’s disease (HD) there is an excess dopamine production and over-activation D1 receptors (D1R) that can produce not only imbalance in dopaminergic neurotransmission but also directly lead to signalling cascades induce cell death. However, simply blocking D1R has important drawbacks as it disrupts several physiological functions. Importantly, many these G-protein coupled (GPCRs) may interact form so called heteromers. These receptor complexes confer...