A5008930203- Parkinson's Disease Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Neurological disorders and treatments
- Nerve injury and regeneration
- Neurotransmitter Receptor Influence on Behavior
- Genetic Neurodegenerative Diseases
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience of respiration and sleep
- Cellular transport and secretion
- Genetics and Neurodevelopmental Disorders
- Cannabis and Cannabinoid Research
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Nuclear Receptors and Signaling
- Sleep and Wakefulness Research
- Regulation of Appetite and Obesity
- Neurological and metabolic disorders
- Neural dynamics and brain function
- Renin-Angiotensin System Studies
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Stress Responses and Cortisol
- Advanced biosensing and bioanalysis techniques
- Rabies epidemiology and control
Universidad de La Laguna
2001-2023
Centre National de la Recherche Scientifique
2011-2015
Université de Bordeaux
2011-2015
Institut des Maladies Neurodégénératives
2010-2015
Universidad de Navarra
2005-2012
Centro de Investigación Biomédica en Red
2010-2012
Biomedical Research Networking Center on Neurodegenerative Diseases
2007-2011
Clinica Universidad de Navarra
2010
Universidade de Santiago de Compostela
2010
Morpho (United States)
2010
The putative presence of the cannabinoid receptor type 2 (CB(2)-R) in central nervous system is still a matter debate. Although first described peripheral and immune tissues, evidence suggesting existence CB(2)-Rs glial cells even neurons has been made available more recently. By taking advantage newly designed CB(2)-R mRNA riboprobes, we have demonstrated by situ hybridization PCR CB2-R transcripts variety brain areas primate Macaca fascicularis, including cerebral cortex hippocampus, as...
Abstract Vesicular glutamate transporters (VGLUTs) are responsible for trafficking and the subsequent regulated release of this excitatory neurotransmitter at synapse. Three isoforms VGLUT have been identified, now known as VGLUT1, VGLUT2, VGLUT3. Both VGLUT1 VGLUT2 considered definitive markers glutamatergic neurons, whereas VGLUT3 is expressed in nonglutamatergic neurons such cholinergic striatal interneurons. It widely believed that a complementary manner cortical thalamic levels,...
Numerous studies suggest that the dopamine transporter (DAT), responsible for reuptake, may act as a vulnerability factor in pathogenesis of Parkinson's disease (PD) and vesicular monoamine (VMAT2), its storage, neuroprotective factor. However, relevance each on differential midbrain DA cells remains unknown. Here we studied relationship between expression pattern (mRNA protein) both transporters model PD (intracerebroventricular injection 6-OHDA rats) monkey human midbrain. Our results...
Abstract Glial cell line‐derived neurotrophic factor (GDNF) is a member of the transforming growth factor‐β superfamily that when exogenously administrated exerts potent trophic action on dopaminergic (DA) cells. Although we know lot about its signalling mechanisms and pharmacological effects, physiological actions GDNF adult brain remain unclear. Here, have used morphological molecular techniques, an experimental model Parkinson's disease in rats, to investigate whether constitutively...
Abstract The position of the caudal intralaminar nuclei within basal ganglia circuitry has largely been neglected in most studies dealing with function. During past few years, there a growing body evidence suggesting that thalamic parafascicular nucleus rodents (PF) exerts multifaceted modulation nuclei, at different levels. Our aim was to study activity thalamostriatal pathway rats unilateral dopaminergic depletion. experimental approach comprised first delivery 6‐OHDA medial forebrain...
Preclinical and clinical data stress the importance of pharmacologically-controlling glial cell line-derived neurotrophic factor (GDNF) intracerebral administration to treat PD. The main challenge is finding a combination genetic switch drug which, when administered at clinically-approved dose, reaches brain in sufficient amounts induce therapeutic effect. We describe highly-sensitive doxycycline-inducible adeno-associated virus (AAV) vector. This vector allowed for first time longitudinal...
Angiotensin II acts via angiotensin type 1 receptors and is a major inducer of inflammation oxidative stress. Local renin-angiotensin systems play role in the development age-related disorders several tissues. These processes are delayed, but not totally abolished, by blockade signaling. A specific receptor for renin its precursor prorenin has recently been identified. We previously showed that neurotoxin-induced dopaminergic (DA) cell loss decreased inhibition receptors, location functional...
Growing evidence shows that autophagy is deficient in neurodegenerative and psychiatric diseases, its induction may have beneficial effects these conditions. However, as shares signaling pathways with cell death interferes protein synthesis, prolonged use of inducers available nowadays considered unwise. The search for novel indicates DRD2 (dopamine receptor 2)-DRD3 ligands also activate autophagy, though critical aspects the action mechanisms dopamine on are still unknown. In order to shed...
Although dopamine has been considered as the only neurotransmitter in nigrostriatal pathway, studies carried out last two decades have suggested existence of a nondopaminergic projection, and more recently, gamma-aminobutyric acid (GABA) identified its neurotransmitter. In this study, we used combination immunocytochemistry for tyrosine hydroxylase (TH; marker dopaminergic neurons), situ hybridization (ISH) different isoforms glutamic decarboxylase (GAD65 GAD67, rate-limiting enzyme GABA...
Aberrant membrane localization of dopamine D 1 receptor (D1R) is associated with l -DOPA-induced dyskinesia (LID), a major complication -DOPA treatment in Parkinson's disease (PD). Since the proteasome plays central role modulating neuronal response through regulation neurotransmitter intraneuronal fate, we hypothesized that ubiquitine-proteasome proteolytic pathway could be impaired LID. Those LIDs are actually striatum-specific decrease catalytic activity and accumulation polyubiquitinated...
Morphine is endogenously synthesized in the central nervous system and endogenous dopamine thought to be necessary for morphine formation. As Parkinson's disease results from loss of associated with pain, we considered how regulated untreated l-DOPA-treated parkinsonian brain. However, as cellular origin overall distribution remains obscure pathological adult brain, first characterized morphine-like compound immunoreactive cells rat striatum. We then studied changes immunoreactivity medium...
Glial cell line-derived neurotrophic factor (GDNF) promotes differentiation, proliferation, and survival in different types, including dopaminergic neurons. Thus, GDNF has been proposed as a promising neuroprotective therapy Parkinson's disease. Although findings from cellular animal models of disease were encouraging, results emerging clinical trials not good expected, probably due to the inappropriate administration protocols. Despite growing information on action mechanisms, many aspects...
Involuntary movements, or dyskinesia, represent a debilitating complication of levodopa therapy for Parkinson's disease. Dyskinesia are, ultimately, experienced by the vast majority patients. Despite importance this problem, little was known about cause situation that has dramatically evolved in last few years with focus upon molecular and signalling changes induced chronic treatment. Departing from this, we here review progress made functional anatomy neuroimaging have had tremendous impact...