A5078892003- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Mast cells and histamine
- Liver Disease and Transplantation
- Neuroscience and Neuropharmacology Research
- Liver Disease Diagnosis and Treatment
- Olfactory and Sensory Function Studies
- Tryptophan and brain disorders
- Polyamine Metabolism and Applications
- Neuropeptides and Animal Physiology
- Gastrointestinal Bleeding Diagnosis and Treatment
- Pharmacological Receptor Mechanisms and Effects
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Gastrointestinal disorders and treatments
- Biomedical Text Mining and Ontologies
- Genomics and Rare Diseases
- Forensic Toxicology and Drug Analysis
- Hepatocellular Carcinoma Treatment and Prognosis
- Bioinformatics and Genomic Networks
- Treatment of Major Depression
- Circadian rhythm and melatonin
- Biosimilars and Bioanalytical Methods
- Genetic Associations and Epidemiology
- Amino Acid Enzymes and Metabolism
Centro de Investigación Biomédica en Red de Salud Mental
2017-2023
Universitat Autònoma de Barcelona
2011-2023
Universidad de Murcia
2022
Instituto de Salud Carlos III
2018-2021
Institute of Research and Innovation Parc Tauli
2021
Vall d'Hebron Institut de Recerca
2017
Medical Research Network
2017
Hospital Clínic de Barcelona
2017
University of California, San Diego
2016
Ferrer Grupo (Spain)
2013-2014
Behavioral and electrophysiological evidence suggests that glutamatergic neurotransmission plays an important role in some of the long-term effects cocaine other drugs abuse on brain function. We therefore examined effect repeated treatment glutamate receptor subunit expression central dopamine (DA) pathways implicated many cocaine's behavioral actions. By immunoblotting procedures using subunit-specific antibodies, we found repeated, but not acute, increased levels immunoreactivity GluR1...
After an episode of acute bleeding from esophageal varices, patients are at high risk for recurrent and death. We compared two treatments to prevent — endoscopic ligation combined medical therapy with nadolol isosorbide mononitrate.
Abstract In previous studies, we have demonstrated that chronic administration of morphine or cocaine produces some common biochemical adaptations in the ventral tegmental area (VTA) and nucleus accumbens (NAc), components mesolimbic dopamine system implicated reinforcing actions these other drugs abuse. Since this neural pathway is also ethanol, it was interest to determine whether ethanol exposure results similar adaptations. Indeed, as seen for treatments, show here treatment increased...
Quantitative blot immunolabeling techniques were used to determine the concentrations of ERK1 (M(r) 44 kDa) and ERK2 42 kDa), two major extracellular signal-regulated protein kinases, in different regions rat brain. The aggregate ERK (ERK1 ERK2) relatively high each brain studied, ranging from approximately 0.35 ng/microgram cerebellum 1.2 nucleus accumbens. However, differences regional distributions resulted ratios their relative abundance that differed by close 10-fold among studied....
Cirrhotic patients with ascites and low ascitic fluid total protein and/or high serum bilirubin levels are at risk to develop the first episode of spontaneous bacterial peritonitis during long–term follow–up. The aim present study was determine efficacy continuous selective intestinal decontamination norfloxacin in prevention this complication. One hundred nine cirrhotic ≤ 1 g/dL or > 2.5 mg/dL without previous were prospectively randomized into two groups: group (n = 56) received...
It is well known that cocaine blocks the dopamine transporter. This mechanism should lead to a general increase in dopaminergic neurotransmission, and yet D(1) receptors (D(1)Rs) play more significant role behavioral effects of than other receptor subtypes. Cocaine also binds σ-1 receptors, physiological which largely unknown. In present study, D(1)R σ(1)R were found heteromerize transfected cells, where robustly potentiated D(1)R-mediated adenylyl cyclase activation, induced MAPK activation...
The role of the pineal gland is to translate rhythmic cycles night and day encoded by retina into hormonal signals that are transmitted rest neuronal system in form serotonin melatonin synthesis release. Here we describe production both regulated a circadian-related heteromerization adrenergic dopamine D₄ receptors. Through α(₁B)-D₄ β₁-D₄ receptor heteromers inhibits signaling blocks induced ligands. This inhibition was not observed at hours when expressed. These data provide new perspective...
Background: The European Bioanalysis Forum dried blood spots (DBS)/microsampling consortium is reporting back from the experiments they performed on further documenting potential hurdles of DBS technology. This paper focused impact hematocrit changes analyses. Results: can have an effect size spot, spot homogeneity and extraction recovery in a compound-dependent manner. change upon aging hematocrit-dependent way. Different card materials give different outcomes. Conclusions: results...
Selective intestinal decontamination with norfloxacin is useful to prevent bacterial infections in several groups of cirrhotic patients at high risk infection. However, the emergence caused by Escherichia coli resistant quinolones has recently been observed undergoing prophylactic norfloxacin. Our aim determine characteristics E. hospitalized patients. One hundred and six 99 were analyzed distributed into two groups: group I (n = 67), sensitive norfloxacin, II 39), The clinical analytical...
Previously, using artificial cell systems, we identified receptor heteromers between the dopamine D(1) or D(2) receptors and histamine H(3) receptor. In addition, demonstrated two biochemical characteristics of receptor-histamine heteromer. We have now extended this work to show heteromer exists in brain serves provide a novel link MAPK pathway GABAergic neurons direct striatal efferent pathway. Using previously, found that ability activation stimulate p44 p42 extracellular signal-regulated...
G protein-coupled receptor (GPCR) heteromers are macromolecular complexes with unique functional properties different from those of its individual protomers. Little is known about what determines the quaternary structure GPCR resulting in their properties. In this study, using resonance energy transfer techniques experiments mutated receptors, we provide for first time clear evidence a key role intracellular domains determination between adenosine A2A, cannabinoid CB1, and dopamine D2...
The general effects of cocaine are not well understood at the molecular level. What is known that dopamine D1 receptor plays an important role. Here we show a key mechanism may be cocaine's blockade histamine H3 receptor-mediated inhibition function. This requires σ1 and occurs upon binding to σ1-D1-H3 complexes. cocaine-mediated disruption leaves uninhibited activates Gs, freely recruits β-arrestin, increases p-ERK 1/2 levels, induces cell death when over activated. Using in vitro assays...
Recent trials have shown that somatostatin (SMT) is as effective sclerotherapy in the treatment of acute variceal bleeding and combination both treatments more than alone. To assess whether addition improves efficacy SMT alone, all patients admitted to our unit with gastrointestinal suspected cirrhosis received a continuous infusion (250 micrograms/h). Endoscopy was performed between 1 5 hours later, esophageal were randomized receive or not sclerotherapy. In groups, continued for days....
We have studied biochemical and behavioral effects of chronic corticosterone administration in two inbred rat stains (Fischer 344 Lewis), known to differ their hypothalamic-pituitary-adrenal axis responses drugs abuse. First, we regulation phosphoproteins the ventral tegmental area sham- corticosterone-treated Fischer Lewis rats, by means back-phosphorylation two-dimensional gel electrophoresis Western blotting analysis. Corticosterone upregulated tyrosine hydroxylase immunoreactivity...
Based on the established role of beta-adrenergic receptor kinase (beta ARK) and beta-arrestin in desensitization several G protein-coupled receptors, we investigated effect chronic morphine administration beta ARK levels selected brain areas. Levels were measured by blot immunolabeling analysis using antibodies specific for two known forms ARK, i.e., ARK1 ARK2. It was found that treatment produced an approximately 35% increase immunoreactivity locus coeruleus, but not other regions studied....
Background: At the start of their work, European Bioanalysis Forum dried blood spots microsampling consortium did not form a dedicated team to investigate spot homogeneity. However, two teams performed experiments that produced results relating sample Results: The data, which were via different approaches (a radiolabeled and nonradiolabeled approach), are highly complementary demonstrate clear effects on inhomogeneity due substrate type, compound hematocrit levels. Conclusion: is significant...
By using double <i>in situ</i> hybridization performed with proenkephalin and H<sub>3</sub>-receptor riboprobes on the same sections from rat brain, we show that histamine H<sub>3</sub>receptors are expressed within striatopallidal neurons of indirect movement pathway. The majority (∼70%) striatal enkephalin express mRNAs. This important degree coexpression mRNAs prompted us to explore effect ligands regulation mRNA expression in striatum. Acute administration ciproxifan, a...
The concentration of 5‐hydroxytryptamine (5‐HT) in rat platelet‐free plasma increased significantly 30 min after a single i.p. injection (10 mg kg −1 ) each six inhibitors the high‐affinity 5‐HT uptake (fluvoxamine, fluoxetine, alaproclate, paroxetine, sertraline and clomipramine). increases ranged from 226% to 776% control values. In contrast, imipramine, desipramine femoxetine had no significant effect. increase elicited by paroxetine was dependent on dose (1, 5 10 returned values 4 h....