- Building materials and conservation
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Conservation Techniques and Studies
- Microbial Applications in Construction Materials
- Calcium Carbonate Crystallization and Inhibition
- Enzyme Production and Characterization
- Cultural Heritage Materials Analysis
- Insect Resistance and Genetics
- Lichen and fungal ecology
- RNA and protein synthesis mechanisms
- Plant and animal studies
- Neurobiology and Insect Physiology Research
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Marine Sponges and Natural Products
- Nanoparticles: synthesis and applications
- Studies on Chitinases and Chitosanases
- Microbial Natural Products and Biosynthesis
- Bee Products Chemical Analysis
- Bacteriophages and microbial interactions
- Slime Mold and Myxomycetes Research
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Plant-Microbe Interactions and Immunity
University of Florence
2015-2025
Google (United States)
2018
University of Chieti-Pescara
1996
ABSTRACT Calcium carbonate precipitation, a widespread phenomenon among bacteria, has been investigated due to its wide range of scientific and technological implications. Nevertheless, little is known the molecular mechanisms by which bacteria foster calcium mineralization. In our laboratory, we are studying calcite formation Bacillus subtilis , in order identify genes involved biomineralization process. A previous screening UV mutants more than one thousand obtained from European B....
Chemical extraction, water retting, microbiological and enzymatic methods were applied on entire nettle stalks and/or unretted decorticated fiber of a selected clone. Morphological mechanical properties chemical composition then determined samples. The first interesting result concerned the good degree separation between fibers shives obtained by scutching stored for 1 year, probably resulting from natural retting processes occurring during storage. Microbiological (anaerobic plus aerobic...
Ruthenium(II) polypyridyl complexes (RPCs) are gaining momentum in photoactivated chemotherapy (PACT), thanks to the possibility of overcoming classical reliance on molecular oxygen photodynamic therapy while preserving selective drug activation by using light. However, notwithstanding intriguing perspectives, translation such an approach development new antimicrobials has been only barely considered. Herein, MTZH-1 and MTZH-2, two novel analogues metronidazole (MTZ), a mainstay treatment...
Two new antibacterial peptides, denominated as Dominulin A and B, have been found on the cuticle in venom of females social paper wasp Polistes dominulus. The amino acidic sequence two determined by mass spectrometry, is INWKKIAE VGGKIL SSL for (MW=1854 Da) INWKKIAEIGKQVL SAL (MW=1909 B. Their presence was confirmed using MALDI-TOF means micro-extractions direct analyses body parts. primary structure dominulins suggest their classification mastoparans, a class peptides other Aculeate...
Summary Pectinolytic microorganisms involved in the water retting process were characterized. Cultivable mesophilic anaerobic and aerobic bacteria isolated from unretted water‐retted material. A total of 104 23 pectinolytic strains identified. Polygalacturonase activity was measured supernatant cell cultures; 24 nine isolates showed an enzymatic higher than reference Clostridium felsineum Bacillus subtilis respectively. We performed first genotypic characterization microflora by a 16S...
Silver nanoparticles (AgNPs) have increasingly gained importance as antibacterial agents with applications in several fields due to their strong, broad-range antimicrobial properties. AgNP synthesis by pulsed laser ablation liquid (PLAL) permits the preparation of stable Ag colloids pure solvents without capping or stabilizing agents, producing AgNPs more suitable for biomedical than those prepared common, wet chemical techniques. To date, only a few investigations into effect produced PLAL...
Weathering processes seriously affect the durability of outdoor marble monuments. In urban environments, a very common deterioration phenomenon is dark discoloration or blackening marble. This paper describes multidisciplinary study on state conservation white marbles Florence Cathedral and microbial community involved in their deterioration. The focused widespread analyzed two differently exposed sites Cathedral. It aims to provide information useful for future interventions control growth....
Although the implications of calcium carbonate (CaCO(3)) precipitation by microorganisms in natural environments are quite relevant, physiology and genetics this phenomenon poorly understood. We have chosen Bacillus subtilis 168 as our model to study which physiological aspects associated with CaCO(3) (calcite) formation during biofilm development when grown on medium. A B. eftA mutant named FBC5 impaired calcite was used for comparative studies. Our results demonstrate that inactivation...
The search for more sustainable strategies to contrast biodeterioration of stone cultural heritage has been developing in recent years find alternatives synthetic biocides, since their toxicity and potential impact on the environment health. In this study, application oregano thyme essential oils (EOs) was tested control microbial growth external marble Florence Cathedral affected by extended darkening. Before situ application, preliminary tests were carried out evaluate interference EOs...
The structural gene of the Proteus mirabilis glutathione transferase GSTB1-1 (gstB) has been isolated from genomic DNA. A nucleotide sequence determination gstB predicted a translational product 203 amino acid residues, perfectly matching previously purified protein [Mignogna, Allocati, Aceto, Piccolomini, Di Ilio, Barra and Martini (1993) Eur. J. Biochem. 211, 421–425]. P. GST revealed 56% identity with Escherichia coli at DNA level 54% identity. Similarity also translation products...
Microorganisms can colonize any known environment and modify it due to their extremely wide versatile metabolism. From the microorganisms point of view, cultural heritage (CH) objects represent a series very heterogeneous habitats (from historical stones paints or archaeological wood) where they grow as well in natural habitats. Aesthetical and/or structural modifications CH materials microbial growth activities are referred biodeterioration. In most cases biodeterioration is not single...