A5059203488- Heat shock proteins research
- Protein Structure and Dynamics
- Advanced Fluorescence Microscopy Techniques
- thermodynamics and calorimetric analyses
- Mitochondrial Function and Pathology
- Photodynamic Therapy Research Studies
- Advanced Polymer Synthesis and Characterization
- Cell Image Analysis Techniques
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- ATP Synthase and ATPases Research
- Lipid Membrane Structure and Behavior
- Photochromic and Fluorescence Chemistry
- Biochemical and Structural Characterization
- Synthetic Organic Chemistry Methods
- Advanced Optical Sensing Technologies
- Advanced Electron Microscopy Techniques and Applications
- Organometallic Complex Synthesis and Catalysis
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
Ludwig-Maximilians-Universität München
2020-2022
Center for Integrated Protein Science Munich
2020-2021
Center for NanoScience
2020
University of Groningen
2018
University of California, Irvine
2015
While buffer cocktails remain the most commonly used method for photostabilization and photoswitching of fluorescent markers, intramolecular triplet-state quenchers emerge as an alternative strategy to impart fluorophores with 'self-healing' or even functional properties such photoswitching. In this contribution, we evaluated combinations both approaches show that inter- quenching processes compete each other. We find although rate is additive, photostability limited by faster pathway. Often...
In recent years, optical microscopy techniques have emerged that allow imaging at unprecedented resolution beyond the diffraction limit. These exploit photostabilizing buffers to enable photoswitching and/or enhancement of fluorophore brightness and stability. A major drawback with use buffers, however, is they cannot be used in live cell imaging. this paper, we tested performance self-healing organic fluorophores, which undergo intramolecular photostabilization, super-resolution examining...
Protein immobilization has gained high interest in recent years for its valuable applications life sciences involving drug delivery and protein arrays. Herein, we combine sortase-mediated with the versatility of magnetic nanoparticles a sensitive GFP-based quantification system. Using this method, successfully immobilized quantified amount coupled enzymes by fluorescence spectroscopy assessed their activity kinetic measurements. We show that coupling enables preparation biological samples...
A supported ruthenium metathesis catalyst misleadingly appears efficient on the basis of ensemble rate data. Nonaveraged single-particle microscopy studies described herein reveal a significant interparticle and intraparticle reactivity heterogeneity potential increasing catalytic efficiency. These SEM, EDS, optical ring-opening polymerization establish mechanism for this spatial distribution in which most molecular centers are catalytically inactive. Further, morphology growing...
Rab GTPases are central regulators of intracellular vesicular trafficking. They frequently targeted by bacterial pathogens through post-translational modifications. Salmonella typhimurium secretes the cysteine protease GtgE during infection, leading to a regioselective proteolytic cleavage regulatory switch I loop in small Rab32 subfamily. Here, using combination biochemical methods, molecular dynamics simulations, NMR spectroscopy, and single-pair Förster resonance energy transfer, we...
Significance The coordinated motion and conformational changes are fundamental for the function of many macromolecules. However, obtaining direct evidence between different subdomains within an individual molecule remains challenging. Here, we apply a single-molecule three-color Förster resonance energy transfer to simultaneously measure three distances single molecule. We utilized this method analyze heat-shock proteins (Hsps). Excitingly, our study provides key differences in cycle Hsp70s...
Abstract While buffer cocktails remain the gold-standard for photostabilization and photoswitching of fluorescent markers, intramolecular triplet-state quenchers emerge as an alternative strategy to impart fluorophores with ‘self-healing’ or even functional properties such photoswitching. In this contribution, we evaluated various combinations both approaches show that inter- quenching processes compete each other rather than being additive synergistic. Often dominate photophysical situation...
Abstract Functional mitochondria are essential for cell viability and depend on protein import from the cytosol. Impaired initiates various well-characterized cellular programs that rescue or remove dysfunctional mitochondria. However, molecular mechanism underlies initial reduction of into defective remained unknown. Here, we found redistribution mtHsp70, mitochondrial chaperone is involved in both folding, regulates efficiency import. During early stress, before initiated membrane...