A5066585085- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Layered Double Hydroxides Synthesis and Applications
- Advanced Photocatalysis Techniques
- RNA modifications and cancer
- Photodynamic Therapy Research Studies
- Photochromic and Fluorescence Chemistry
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Click Chemistry and Applications
- 3D Printing in Biomedical Research
- Supramolecular Self-Assembly in Materials
- Enzyme-mediated dye degradation
- Cancer Cells and Metastasis
- Chemical Synthesis and Analysis
- Cyclopropane Reaction Mechanisms
- Polyamine Metabolism and Applications
- Porphyrin and Phthalocyanine Chemistry
- Antibiotics Pharmacokinetics and Efficacy
- Microbial Natural Products and Biosynthesis
University of Groningen
2013-2024
University Medical Center Groningen
2020-2024
Dialyse Centrum Groningen
2018
Abstract Layered double hydroxides (LDHs) have been widely studied for biomedical applications due to their excellent properties, such as good biocompatibility, degradability, interlayer ion exchangeability, high loading capacity, pH‐responsive release, and large specific surface area. Furthermore, the flexibility in structural composition ease of modification LDHs makes it possible develop specifically functionalized meet needs different applications. In this review, recent advances...
Topography-driven alterations to single cell stiffness rather than in morphology, is the underlying driver for influencing biological processes, particularly stem differentiation.
Abstract The loading capacity and strategy of layered double hydroxides (LDHs) determine their suitability for drug delivery applications. In this study, two sizes MgAl‐CO 3 LDHs are converted into MgAl‐NO MgAl‐Cl by an acid‐salt method. influence the size type interlayer anions on studied compared. Here, Methylene Orange (MO) served as anionic structure to capacity. Due strongest anion‐exchange NO − larger distance LDHs, they have impressive high capacities, which make these useful...
Multifunctional nanogel coatings provide a promising antimicrobial strategy against biomedical implant-associated infections. Nanogels can create hydrated surface layer to promote antifouling properties effectively. Further modification of nanogels with quaternary ammonium compounds (QACs) potentiates activity owing their positive charges along the presence membrane-intercalating alkyl chain. This study effectively demonstrates that poly(N-isopropylacrylamide-
While buffer cocktails remain the most commonly used method for photostabilization and photoswitching of fluorescent markers, intramolecular triplet-state quenchers emerge as an alternative strategy to impart fluorophores with 'self-healing' or even functional properties such photoswitching. In this contribution, we evaluated combinations both approaches show that inter- quenching processes compete each other. We find although rate is additive, photostability limited by faster pathway. Often...
Covalent linkage of fluorophores and photostabilizers was recently revived as a strategy to make organic “self-healing” <italic>via</italic> triplet-state quenching. Although Lüttke co-workers pioneered this already in the 1980s, general design principles still remain elusive. In contribution, we combine experiments theory understand what determines photostabilization efficiency dye–photostabilizer conjugates. Our results from single-molecule microscopy molecular dynamics simulations...
Single-molecule fluorescence microscopy studies of bacteria provide unique insights into the mechanisms cellular processes and protein machineries in ways that are unrivalled by any other technique. With cost microscopes dropping availability fully automated microscopes, volume data produced has increased tremendously. These developments have moved bottleneck throughput from image acquisition sample preparation to analysis. Furthermore, requirements for analysis procedures become more...
The continuous emergence of antimicrobial resistance is causing a threat to patients infected by multidrug-resistant pathogens. In particular, the clinical use aminoglycoside antibiotics, broad-spectrum antibacterials last resort, limited due rising bacterial resistance. One major mechanisms in Gram-positive and Gram-negative bacteria phosphorylation these amino sugars at 3'-position O-phosphotransferases [APH(3')s]. Structural alteration antibiotics would be an obvious strategy tackle this...
Site-selective diazotation: A facile one-step modification at the 2-desoxystreptamine ring, is reported in diverse neamine antibiotics reaching high regioselectivities (see scheme; r.s.=regioselectivities). The diazo-transfer reaction takes place aqueous solution, under mild conditions, and without any protective groups. As a service to our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may be re-organized for online...
Engineered viruses are finding an increasing number of applications in basic, translational research and materials science. Genetic chemical engineering capsids represents a key point for tailoring the properties viral particles, but synthetic efforts limits accompanying these processes still hinder their usability. Here, single-step highly selective biocatalytic functionalization approach is described, providing general platform virus-acrylate hybrid particles. The tobacco mosaic virus...
Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present modular that trans-encoded based on tRNA-mimicking structures (TMSs). These provide a high degree freedom for reengineering can thus be designed accept wide range inputs, including RNA, small molecules, proteins. This powerful approach enables control the translation protein expression plasmid genome DNA.
Abstract While buffer cocktails remain the gold-standard for photostabilization and photoswitching of fluorescent markers, intramolecular triplet-state quenchers emerge as an alternative strategy to impart fluorophores with ‘self-healing’ or even functional properties such photoswitching. In this contribution, we evaluated various combinations both approaches show that inter- quenching processes compete each other rather than being additive synergistic. Often dominate photophysical situation...
Abstract Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present modular that trans‐encoded based on tRNA‐mimicking structures (TMSs). These provide a high degree freedom for reengineering can thus be designed accept wide range inputs, including RNA, small molecules, proteins. This powerful approach enables control the translation protein expression plasmid genome DNA.
Abstract Single-molecule fluorescence microscopy studies of bacteria provide unique insights into the mechanisms cellular processes and protein machineries in ways that are unrivalled by any other technique. With cost microscopes dropping availability fully automated microscopes, volume data produced has increased tremendously. These developments have moved bottleneck throughput from image acquisition sample preparation to analysis. Furthermore, requirements for analysis procedures become...