A5073575998- Cell Adhesion Molecules Research
- Peroxisome Proliferator-Activated Receptors
- Diet, Metabolism, and Disease
- Biotin and Related Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Alcohol Consumption and Health Effects
- Immune Response and Inflammation
- Receptor Mechanisms and Signaling
- Liver Disease Diagnosis and Treatment
- Pharmacological Receptor Mechanisms and Effects
- Adipose Tissue and Metabolism
- Asthma and respiratory diseases
- Inflammatory mediators and NSAID effects
- Pancreatic function and diabetes
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Photoreceptor and optogenetics research
- Erythrocyte Function and Pathophysiology
- Retinoids in leukemia and cellular processes
- Retinal Development and Disorders
- Eicosanoids and Hypertension Pharmacology
- Neuroinflammation and Neurodegeneration Mechanisms
Massachusetts General Hospital
2013-2021
Harvard University
2013-2021
CD47 plays an important but incompletely understood role in the innate and adaptive immune responses. CD47, also called integrin-associated protein, has been demonstrated to associate cis with β1 β3 integrins. Here we test hypothesis that regulates adhesive functions of T-cell α4β1 (VLA-4) αLβ2 (LFA-1) vivo vitro models inflammation. Intravital microscopy studies reveal −/− Th1 cells exhibit reduced interactions wild-type (WT) inflamed cremaster muscle microvessels. Similarly, murine cells,...
Metabolic studies suggest that the absorptive capacity of small intestine for fructose is limited, though molecular mechanisms controlling this process remain unknown. Here we demonstrate thioredoxin-interacting protein (Txnip), which regulates glucose homeostasis in mammals, binds to transporters and promotes absorption by intestine. Deletion Txnip mice reduced transport into peripheral bloodstream liver, as well severity adverse metabolic outcomes resulting from long-term consumption. We...
Abstract Antibody ligation of the murine neutrophil surface protein Ly6G disrupts migration in some contexts but not others. We tested whether this variability reflected divergent dependence on β2 integrins, adhesion molecules that interact with at surface. In integrin-dependent arthritis, attenuated joint inflammation, even though mice lacking altogether developed arthritis normally. By contrast, had no impact integrin-independent into inflamed lung. peritoneum, role integrins varied...
The initial steps in the synthesis of leukotrienes are translocation 5-lipoxygenase (5-LO) to nuclear envelope and its subsequent association with scaffold protein 5-lipoxygenase-activating (FLAP). A major gap our understanding this process is knowledge how organization 5-LO FLAP on regulates leukotriene synthesis. We combined single molecule localization microscopy Clus-DoC cluster analysis, also a novel unbiased analysis analyze changes relationships between response activation RBL-2H3...
Increased consumption of fats and added sugars has been associated with an increase in metabolic syndromes. Here we show that mice chronically fed energy-rich diet (ERD) high fat moderate sucrose have enhanced the absorption a gastrointestinal fructose load, this required expression arrestin domain protein Txnip intestinal epithelial cells. ERD feeding induced gene Glut5, Txnip. Furthermore, interacted Rab11a, small GTPase facilitates apical localization Glut5. We also demonstrate promoted...
Over the last several years it has become clear that higher order assemblies on membranes, exemplified by signalosomes, are a paradigm for regulation of many membrane signaling processes. We have recently combined two-color direct stochastic optical reconstruction microscopy (dSTORM) with (Clus-DoC) algorithm combines cluster detection and colocalization analysis to observe organization 5-lipoxygenase (5-LO) 5-lipoxygenase-activating protein (FLAP) into nuclear envelope mast cells; these...
Abstract Antibody ligation of the murine neutrophil surface protein Ly6G disrupts migration in some contexts but not others. We tested whether this variability reflected divergent dependence on β2 integrins, adhesion molecules that interact with at surface. In integrin-dependent arthritis, attenuated joint inflammation, even though mice lacking altogether developed arthritis normally. By contrast, had no impact integrin- independent into inflamed lung. peritoneum, role integrins varied...
Abstract The initial steps in the synthesis of leukotrienes are translocation 5-lipoxygenase (5-LO) to nuclear envelope and its subsequent association with scaffold protein 5-lipoxygenase-activating (FLAP). A major gap our understanding this process is knowledge how organization 5-LO FLAP on regulates leukotriene synthesis. We combined single molecule localization microscopy Clus-DoC cluster analysis, also a novel unbiased analysis analyze changes relationships between response activation...
Abstract Immune cells must integrate multiple extracellular signals to produce an appropriate inflammatory response, including production of a dynamic mix eicosanoids and related bioactive lipids. Synthesis these lipids is initiated on the membrane surface nuclear envelope endoplasmic reticulum. One critical question how precursor arachidonic acid (AA) distributed between initial biosynthetic enzymes arachidonate 5-lipoxygenase (5-LO) prostaglandin-endoperoxidase synthase-1/2 (COX-1/2)...