A5103128471- DNA Repair Mechanisms
- Antimicrobial Peptides and Activities
- Erythrocyte Function and Pathophysiology
- Inflammation biomarkers and pathways
- Chronic Obstructive Pulmonary Disease (COPD) Research
- DNA and Nucleic Acid Chemistry
- Voice and Speech Disorders
- Genetic Neurodegenerative Diseases
- Genomics and Chromatin Dynamics
- Dysphagia Assessment and Management
- Parkinson's Disease Mechanisms and Treatments
- Carcinogens and Genotoxicity Assessment
- Pediatric health and respiratory diseases
- Historical and Contemporary Political Dynamics
- Empathy and Medical Education
- Polydiacetylene-based materials and applications
- Neuroscience and Neuropharmacology Research
- Acute Myocardial Infarction Research
- Respiratory viral infections research
- Balance, Gait, and Falls Prevention
- Art Therapy and Mental Health
- Neuroscience of respiration and sleep
- Neonatal and Maternal Infections
- Thyroid Disorders and Treatments
- Immune Response and Inflammation
University College Dublin
2019-2024
Istituto Oncologico Veneto
2016-2020
Rutgers, The State University of New Jersey
2003-2019
Istituti di Ricovero e Cura a Carattere Scientifico
2016
Rutgers New Jersey Medical School
1999-2009
Inserm
2001
Centre National de la Recherche Scientifique
2001
Institut de génétique et de biologie moléculaire et cellulaire
2001
University of South Alabama
2001
ABSTRACT Human gingival epithelial cells (GEC) produce peptides, such as β-defensins and the cathelicidin LL-37, that are both antimicrobial modulate innate immune response. In myeloid airway cells, active form of vitamin D 3 [1,25(OH) 2 ] increases expression antibacterial activity LL-37. To examine on defense epithelium, cultured were treated with either 10 −8 M 1,25(OH) or ethanol for up to 24 h. A time-dependent induction LL-37 mRNA 13-fold at h in standard monolayer three-dimensional...
The events responsible for repair of DNA interstrand cross-links in mammalian cells, the proteins involved and their interactions with each other are poorly understood. present study demonstrates that structural protein nonerythroid alpha spectrin (alphaSpIISigma*), normal human cell nuclei, plays an important role cross-links. These results show alphaSpIISigma* relocalizes to nuclear foci after damage cells cross-linking agent 8-methoxypsoralen plus ultraviolet A (UVA) light FANCA known XPF...
Fanconi anemia (FA) is a genetic disorder characterized by bone marrow failure, congenital abnormalities, cancer susceptibility, and marked cellular hypersensitivity to DNA interstrand cross-linking agents, which correlates with defect in ability repair this type of damage. We have previously identified an approximately 230-kDa protein present nuclear complex normal human lymphoblastoid cells that involved cross-links shows reduced levels FA-A cell nuclei. The FANCA gene appears play role...
The airway epithelium plays a role in host defense through the binding of innate immune receptors, which leads to activation inflammatory mediators, including antimicrobial peptides. active form vitamin D, 1,25(OH) 2 D 3 , induces expression peptide LL-37 both myeloid cells and epithelial (AEC). Here, we demonstrate that mRNA encoding triggering receptor expressed on (TREM)-1 was induced up 12-fold by normal human bronchial (NHBE) well-differentiated cultures six cell lines from patients...
Repair of DNA interstrand cross-links is a complex process critical to which the identification sites damage by specific proteins. We have recently identified structural protein nonerythroid α spectrin (αSpIIΣ*) as component nuclear in normal human cells involved repair and shown that it forms with Fanconi anemia proteins FANCA, FANCC, FANCG. Using affinity chromatography, we now show αSpIIΣ*, present HeLa cell nuclei, specifically binds containing psoralen FANCG are bound this damaged well....
Background and Objective Both chronic aggressive periodontal disease are associated with vitamin D deficiency. The active form of D, 1,25(OH) 2 3 , induces the expression antimicrobial peptide LL‐37 innate immune mediators in cultured human gingival epithelial cells (GECs). aim this study was to further delineate mechanism by which enhances defense against development (PD). Materials Methods Wild‐type C57Bl/6 mice were made deficient dietary restriction. Cultured primary immortalized GEC...
Nonerythroid alpha-spectrin (alphaSpIISigma( *)) is a structural protein that has been identified in the nucleus of mammalian cells and shown to be involved DNA repair. It also deficient from clinically diverse genetic disorder Fanconi anemia (FA). In order get clearer understanding role alphaSpIISigma( *) repair, whether it may have other important functions nucleus, studies were undertaken identify specific binding partners nucleus. The results demonstrate multiple proteins...
Repair of DNA interstrand cross-links is a multistep process, critical to which production incisions at the site lesion resulting in unhooking cross-link from DNA. We have previously shown that XPF involved psoralen and Fanconi anemia, complementation group A (FA-A) cells, there deficiency these incisions. now demonstrate FA B, C, D2, F, G cells also Involvement proteins this process demonstrated by ability corrected with appropriate FANC cDNAs, produce inhibition antibodies against...
Respiratory disorders are the most common cause of death in Parkinson's Disease (PD). Conflicting data exist on aetiology respiratory dysfunction PD and few studies examine effects exercise-based interventions measures. This study was conducted to better understand identify measures responsive an integrative exercise programme.
Cells from individuals with the cancer-prone, inherited disorder Fanconi anemia (FA) are hypersensitive to DNA interstrand cross-linking agents and this hypersensitivity correlates a defect in ability repair type of damage their DNA. We have isolated endonuclease complex nuclei normal human cells which is involved cross-links shown that FA complementation group A (FA-A) there incise containing cross-links. In order identify specific protein(s) defective FA-A cells, monoclonal antibodies...
Parkinson's Disease (PD) has the second-highest prevalence rate of all neurodegenerative disorders. It effects approximately 1% population over age 60, with this proportion rising further, in more elderly cohorts. PD manifests as several motor and non-motor disfunctions, which develop progressively time. Gait mobility problems are amongst most debilitating symptoms for people PD. They severely affect a person's ability to carry out daily activities living can lead decreased quality life....
Background The availability of bare metal stents (BMS) followed by drug-eluting first- (DES1) and second-generation (DES2) progressively increased the rate percutaneous revascularizations [percutaneous coronary intervention (PCI)] with unknown impact on long-term outcome real-world patients established artery disease. We sought to investigate treatments applied in disease BMS, DES1 DES2 eras their 5-year outcome. Methods A total 3099 consecutive at least one stenosis more than 50% observed...
Abstract β-defensins act as antimicrobial agents and immunomodulators. Human β-defensin (BD) peptides 1, 2 3 are differentially modulated in primary tracheal epithelial cells (TEC) by viruses, bacteria, proinflammatory cytokines. We hypothesized that hBD-1 virulence factors would influence induction of hBD-2 hBD-3 during lung bacterial infections. Since BD genes exist close proximity on chromosome 8, making it impossible to delete more than one gene, a model was devised study their...
Studies in our laboratory demonstrate a role for nonerythroid α spectrin (αSpII∑*) human cell nuclei as well new function it the cell, an involvement DNA repair, particular, repair of interstrand cross-links. In cells treated with cross-linking agent, αSpII∑* co-localizes damage-induced nuclear foci XPF, protein involved cross-link repair. It also co-immunoprecipitates XPF. HeLa and purified bovine brain bind to containing Also, anti-αSpII∑* decreases levels incisions produced by normal...