A5091189717- Genetics, Aging, and Longevity in Model Organisms
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Circadian rhythm and melatonin
- Reproductive Biology and Fertility
- Photosynthetic Processes and Mechanisms
- Pluripotent Stem Cells Research
- Redox biology and oxidative stress
- Spaceflight effects on biology
- CRISPR and Genetic Engineering
- Skin and Cellular Biology Research
- Fungal and yeast genetics research
- Erythrocyte Function and Pathophysiology
- Neurological disorders and treatments
- Polysaccharides and Plant Cell Walls
- DNA Repair Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Adipose Tissue and Metabolism
- Developmental Biology and Gene Regulation
- Muscle Physiology and Disorders
- Plant Stress Responses and Tolerance
- Tissue Engineering and Regenerative Medicine
- Heat shock proteins research
University of Florida
2025
Inserm
2001-2022
Université de Strasbourg
1998-2022
Centre National de la Recherche Scientifique
2001-2022
Institut de génétique et de biologie moléculaire et cellulaire
2001-2022
Institut de Biologie Paris-Seine
2019
Institut de Biologie Moléculaire et Cellulaire
2016
Génétique Moléculaire Génomique Microbiologie
2015
Institut Clinique de la Souris
2015
Max Planck Institute of Molecular Cell Biology and Genetics
2003-2007
To build complex organs, embryos have evolved mechanisms that integrate the development of cells unrelated to one another by cell type or ancestry. Here we show pha-4 locus establishes organ identity for Caenorhabditis elegans pharynx. In mutants, pharyngeal are transformed into ectoderm. Conversely, ectopic expression produces excess cells. encodes an HNF-3 homolog selectively expressed in nascent digestive tract, including all pharynx precursors at time they restricted a fate. We suggest...
Myosin II plays a central role in epithelial morphogenesis; however, its has mainly been examined processes involving single cell type. Here we analyze the structure, spatial requirement and regulation of myosin during C. elegans embryonic elongation, process that involves distinct epidermal cells muscles. We developed novel GFP probes to visualize dynamics actomyosin remodeling, found assembly filaments, but not actin microfilaments, depends on regulatory light chain (MLC-4) essential...
Non-centrosomal microtubule arrays assemble in differentiated tissues to perform mechanical and transport-based functions. In this study, we identify Caenorhabditis elegans NOCA-1 as a protein with homology vertebrate ninein. contributes the assembly of non-centrosomal multiple tissues. larval epidermis, functions redundantly minus end protection factor Patronin/PTRN-1 circumferential array essential for worm growth morphogenesis. Controlled degradation γ-tubulin complex subunit tissue...
Embryonic morphogenesis involves the coordinate behaviour of multiple cells and requires accurate balance forces acting within different through application appropriate brakes throttles. In C. elegans, embryonic elongation is driven by Rho-binding kinase (ROCK) actomyosin contraction in epidermis. We identify an evolutionary conserved, actin microfilament-associated RhoGAP (RGA-2) that behaves as a negative regulator LET-502/ROCK. The small GTPase RHO-1 preferred target RGA-2 vitro, acts...
In synucleinopathies, including Parkinson's disease (PD), dopamine neurons in the substantia nigra pars compacta (SNc) exhibit greater vulnerability to degeneration than those ventral tegmental area (VTA). While α-synuclein (αSyn) pathology is implicated nigral neuron loss, mechanisms by which αSyn affects neuronal activity and midbrain network connectivity prior cell death remain unclear. This study tested hypothesis that elevated expression induces pathophysiological changes firing...
C. elegans embryonic elongation is a morphogenetic event driven by actomyosin contractility and muscle-induced tension transmitted through hemidesmosomes. A role for the microtubule cytoskeleton has also been proposed, but its contribution remains poorly characterized. Here, we investigate organization of non-centrosomal arrays present in epidermis assess their function elongation. We show that regulators γ-tubulin NOCA-1 are recruited to hemidesmosomes adherens junctions early Several...
Cytoskeletal regulation is important in cell migration. The Caenorhabditis elegans gonadal distal tip cells (DTCs) offer a simple model with which to investigate the mechanism of migration organogenesis. Here, we report that one spectraplakin isoforms, VAB-10B1, plays an essential role and nuclear DTCs by regulating actin microtubule (MT) cytoskeleton. In vab-10(tk27) mutant, lacks alignment filamentous (F)-actin MTs was weakly severely disorganized, respectively, resulted failure...
Mechanical forces can elicit a mechanotransduction response through junction-associated proteins. In contrast to the wealth of knowledge available for focal adhesions and adherens junctions, much less is known about at hemidesmosomes. Here, we focus on C. elegans plectin homolog VAB-10A, only evolutionary conserved hemidesmosome component. elegans, muscle contractions induce pathway in epidermis We used CRISPR precisely remove spectrin repeats (SRs) or partially hidden Src homology 3 (SH3)...
Environmental oxidative stress threatens cellular integrity and should therefore be avoided by living organisms. Yet, relatively little is known about environmental perception. Here, using microfluidics, we showed that like I2 pharyngeal neurons, the tail phasmid PHA neurons function as sensing in C . elegans , but display different responses to H 2 O light. We uncovered related receptors, GUR-3 LITE-1, mediate signaling neurons. Still, peroxiredoxin PRDX-2 essential for both, might promote...
Abstract lin-26, which encodes a unique Zn-finger protein, is required for differentiation of nonneuronal ectodermal cells in Caenorhabditis elegans. Here, we show that the two genes located immediately upstream lin-26 encode LIN-26-like proteins; hence their names are lir-1 and lir-2 (lin-26 related). lir-2, lir-1, generate several isoforms by alternative splicing and/or trans-splicing at different positions. On basis pattern, intergenic distances, expression, suggest form overlapping...
C. elegans embryonic elongation is a morphogenetic event driven by actomyosin contractility and muscle-induced tension transmitted through hemidesmosomes. A role for the microtubule cytoskeleton has also been proposed, but its contribution remains poorly characterized. Here, we investigate organization of non-centrosomal arrays present in epidermis assess their function elongation. We show that regulators γ-tubulin NOCA-1 are recruited to hemidesmosomes adherens junctions early Several...
Perception of oxidative stress in nematodes involves specific neurons expressing antioxidant enzymes. Here, we carefully characterized GFP knock-in lines for C. elegans peroxiredoxin PRDX-2 and thioredoxin TRX-1, uncovered that left right I2, PHA ASJ reproducibly express an asymmetric level each enzyme. We observed high-expressing are most cases associated with a particular side, indicating directional rather than stochastic type asymmetry. propose the biological relevance this left-right...
Abstract Environmental oxidative stress threatens cellular integrity and should therefore be avoided by living organisms. Yet, relatively little is known about environmental perception. Here, using microfluidics, we showed that like I2 pharyngeal neurons, the tail phasmid PHA neurons function as sensing in C. elegans , but display different responses to H 2 O light. We uncovered related receptors, GUR-3 LITE-1, mediate signaling neurons. Still, peroxiredoxin PRDX-2 essential for both, might...
Abstract Mechanical forces control many cellular processes by eliciting a mechanotransduction response in target cells. The initial steps of at hemidesmosomes remain undefined contrast to focal adhesions and adherens junctions. Here, we focus on the C. elegans plectin homolog VAB-10A, only evolutionary conserved hemidesmosome component. In , muscle contractions induce pathway epidermis through hemidesmosomes. We used CRISPR precisely remove spectrin repeats (SR) or partially hidden...