One of the main limitations to Pt-therapy in cancer is development associated drug resistance that can be with a significant reduction intracellular platinum concentration. Thus, Pt concentration could considered as biomarker cisplatin resistance. In this work, an alternative method address individual cells explored permit evaluation different cell models and therapies relatively fast way. For aim, total analysis single has been implemented using consumption nebulizer coupled inductively...

Several organisms have demonstrated the ability of synthesising biogenic selenium-containing nanoparticles. Such particles from biological sources attracted great attention due to several proven activities as antioxidants or antimicrobial agents. However, little is known in terms size (distribution), shapes, chemical composition and number/amount/concentration these particles. Therefore, this work, we proposed use complementary analytical strategies that enabled detection characterization...

Cells are able to precisely control the amount of iron they acquire in form transferrin (TF)-bound by modulating synthesis receptor 1 (TfR1). In tumor cells, elevated TfR1 seems be related poorer outcome for patients. Thus, direct measurement this biomarker breast cancer tissues and cells might serve as a prognosis biomarker. work, we have used Nd-labeled antibodies tag present on cell surface two models with different malignancy (MCF7 MDA-MB 231). For aim, monoclonal antibody anti-TfR1 is...

The objective of this study was to evaluate the accumulation ions in blood and organs caused by titanium (Ti) metal particles a mandibular defect rats, together with description local reaction oral tissues Ti alloy debris.Twenty Sprague-Dawley rats were randomly distributed into three groups: an experimental group bone filled metallic debris obtained implantoplasty; positive control group; negative group. Thirty days after surgery, euthanized perilesional tissue surrounding removed, lungs,...

<title>Abstract</title> MicroRNAs (miRNAs) are short single stranded RNA sequences that play an important role in the initiation and progression of cancer. Therefore, present work tries to establish analytical platform for quantitative analysis this miRNA cancer cell models without enzymatic amplification reactions. The developed assay is based on a sandwich double-hybridization reaction using capture oligonucleotide conjugated magnetic iron oxide microparticles detection 40 nm gold...

More than a decade ago, studies on cellular cisplatin accumulation

This work studies the presence of Ti, Al and V metal ions Ti nanoparticles released from debris produced by implantoplasty, a surgical procedure used in clinic, rat organs.The sample preparation for total determination was carefully optimized using microsampling inserts to minimize dilution during acid attack lyophilized tissues microwave-assisted digestion method. An enzymatic method applied different tissue samples order extract titanium single-particle ICP-MS analysis.A statistically...

Cisplatin, one of the most extensively used metallodrugs in cancer treatment, presents important drawback patient resistance. This resistance is consequence different processes including those preventing formation DNA adducts and/or their quick removal. Thus, a tool for accurate detection and quantitation cisplatin-induced might be valuable predicting To prove validity such an assumption, highly sensitive plasma mass spectrometry (ICP-MS) strategies were applied to determine adduct levels...

Streptomycetes are important biotechnological bacteria with complex differentiation. Copper is a well-known positive regulator of differentiation and antibiotic production. However, the specific mechanisms buffering cytosolic copper biochemical pathways modulated by remain poorly understood. Here, we developed new methodology to quantify in single spores which allowed us propose that modulates asynchrony germination. We also characterised SCO2730/2731 chaperone/P-type ATPase export system. A...

MicroRNAs (miRNAs), which are short single-stranded RNA sequences between 18 and 24 nucleotides, known to play a crucial role in gene expression. Changes their expression not only involved many diseases but also as response physiological changes, such physical exercise. In this work, new analytical strategy for the sensitive specific analysis of miRNA human plasma is presented. The developed does depend on any nucleic acid amplification process can be obtained direct correlation number...

Streptomyces are important soil bacteria used for bioremediation of metal-contaminated soils, however, it is still unknown how metal-selective and which mechanisms involved during their capture. In this work, we exposed S. coelicolor spores to environmentally relevant concentrations (0.1, 1, 10, 100 μM) Ce, U Cd in solid medium one week investigate the uptake behaviour hyphae newly formed spores. Additionally, metal adsorption onto was explored by incubating inactive, ungerminated day...

In the search for a normalized procedure to replicate and compare single cell-inductively coupled plasma-mass spectrometry (SC-ICP-MS) experiments, SELM-1, certified reference material containing selenium enriched yeast cells has been used. Selenium concentrations (both, intra- extracellular) have measured using either sequential or simultaneous procedures. Regarding quantitative results, involving cell washing followed by freeze drying of washed intracellular Se quantification SC-ICP-MS...

Streptomycetes are bacteria of significant biotechnological interest due to their production bioactive specialised metabolites used in medicine and agriculture. In these bacteria, metabolism morphological differentiation linked typically repressed under high phosphate conditions. This study characterises a DeoR-like transcriptional regulator, SCO1897, Streptomyces coelicolor, whose expression increases during sporulation. Disruption sco1897 results reduced biosynthesis metabolites, delayed...

Copper modulates secondary metabolism in Streptomyces. Although the cytosolic copper concentration is controlled by several chaperones and transporters, formation of nanoparticles (NPs) its relation to antibiotic production has never been established model Streptomyces coelicolor. In this work, state-of-the-art analytical tools are used evaluate incorporation individual spores S. coelicolor at different exposure concentrations (40, 80, 160 μM Cu). Among them, use single cell-inductively...

Titanium oxide nanoparticles released by titanium implants can be detected and characterized single-particle ICP-MS transmission electron microscopy.