A5020184372- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- Lymphoma Diagnosis and Treatment
- T-cell and Retrovirus Studies
- Genetic Associations and Epidemiology
- Gene expression and cancer classification
- Chronic Lymphocytic Leukemia Research
- Plant-Microbe Interactions and Immunity
- Folate and B Vitamins Research
- Neurological diseases and metabolism
- Bioinformatics and Genomic Networks
- Evolution and Genetic Dynamics
- Genetics and Plant Breeding
- Animal Genetics and Reproduction
- Mycotoxins in Agriculture and Food
- Genetic Syndromes and Imprinting
- Plant Disease Resistance and Genetics
- Molecular Biology Techniques and Applications
- Genetic diversity and population structure
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Genetics, Bioinformatics, and Biomedical Research
- Virus-based gene therapy research
- Genomics and Rare Diseases
- Cytokine Signaling Pathways and Interactions
North Carolina State University
2010-2025
North Central State College
2014
Translational Research Informatics Center (Japan)
2013-2014
University of North Carolina at Chapel Hill
2013
Duke University
2005-2013
Center for Translational Molecular Medicine
2013
UNC Lineberger Comprehensive Cancer Center
2013
Center for Genomic Science
2013
Duke University Hospital
2005
Duke Medical Center
2005
Abstract We have exploited "progeny testing" to map quantitative trait loci (QTL) underlying the genetic variation of milk production in a selected dairy cattle population. A total 1,518 sires, with progeny tests based on milking performances > 150,000 daughters jointly, was genotyped for 159 autosomal microsatellites bracketing 1645 centimorgan or approximately two thirds bovine genome. Using maximum likelihood multilocus linkage analysis accounting variance heterogeneity phenotypes,...
Estimates of genetic population structure ( F ST ) were constructed from all autosomes in two large SNP data sets. The Perlegen set contains genotypes on ∼1 million SNPs segregating three samples Americans African, Asian, and European descent; the Phase I HapMap ∼0.6 four specific Caucasian, Chinese, Japanese, Yoruba populations. Substantial heterogeneity values was found between segments within chromosomes, although there similarity There also substantial among population-specific values,...
An inheritable muscular hypertrophy was recently described in sheep and shown to be determined by the callipyge gene mapped ovine chromosome 18. Here, phenotype found characterized a nonmendelian inheritance pattern, referred as polar overdominance, where only heterozygous individuals having inherited mutation from their sire express phenotype. The possible role of parental imprinting determinism overdominance is envisaged.
A mutation causing muscular hypertrophy, with associated leanness and improved feed efficiency, has been recently identified in domestic sheep (Ovis aries). Preliminary results indicate that an autosomal dominant gene may be responsible for this economically advantageous trait. We have exploited the conservation sequence chromosomal location of DNA markers across Bovidae to map corresponding callipyge locus ovine chromosome 18 using a battery bovine 21 markers. Chromosomal localization is...
Abstract Diffuse large B-cell lymphoma (DLBCL) is the most common subtype, and fewer than half of patients are cured with standard first-line therapy. To improve therapeutic options, better animal models that accurately mimic human DLBCL (hDLBCL) needed. Canine DLBCL, one cancers in veterinary oncology, morphologically similar to hDLBCL treated using chemotherapeutic protocols. With genomic technologies, it now possible molecularly evaluate dogs as a potential large-animal model for hDLBCL....
A genetic disease in cattle, progressive degenerative myeloencephalopathy (weaver disease), is associated with increased milk production. This association could result from population stratification, a pleiotropic effect of single gene, or linkage disequilibrium between the gene causing weaver and quantitative trait locus (QTL) for To test these hypotheses, we performed an extensive study bovine pedigree segregating condition identified microsatellite (TGLA116) closely linked to (zmax, 8.15;...
Wing pattern evolution in Heliconius butterflies provides some of the most striking examples adaptation by natural selection. The genes controlling variation are classic Mendelian loci large effect, where allelic causes and discrete phenotypic changes is responsible for both convergent highly divergent wing across genus. We characterize nucleotide variation, genotype-by-phenotype associations, linkage disequilibrium (LD), candidate gene expression patterns two unlinked genomic intervals that...
Identifying the genetic changes driving adaptive variation in natural populations is key to understanding origins of biodiversity. The mosaic mimetic wing patterns Heliconius butterflies makes an excellent system for exploring using next-generation sequencing. In this study, we use a combination techniques annotate genomic interval modulating red color pattern variation, identify narrow region responsible divergence and convergence patterns, explore evolutionary history these alleles. We...
Maize kernels are susceptible to infection by the opportunistic pathogen Aspergillus flavus. Infection results in reduction of grain quality and contamination with highly carcinogenic mycotoxin, aflatoxin. To understanding host response fungus, transcription approximately 9,000 maize genes were monitored during host-pathogen interaction a custom designed Affymetrix GeneChip® DNA array. More than 1,000 found differentially expressed at fold change 2 or greater. This included up regulation...
Abstract Osteosarcoma (OS) is the most commonly diagnosed malignant bone tumor in humans and dogs, characterized both species by extremely complex karyotypes exhibiting high frequencies of genomic imbalance. Evaluation signatures human OS using array comparative hybridization (aCGH) has assisted uncovering genetic mechanisms that result disease phenotype. Previous low‐resolution (10–20 Mb) aCGH analysis canine identified a wide range recurrent DNA copy number aberrations, indicating...
Much remains unknown of molecular events controlling the plant hypersensitive defense response (HR), a rapid localized cell death that limits pathogen spread and is mediated by resistance (R-) genes. Genetic control HR hard to quantify due its microscopic nature. Natural modifiers ectopic phenotype induced an aberrant auto-active R-gene (Rp1-D21), were mapped in population 3,381 recombinant inbred lines from maize nested association mapping population. Joint linkage analysis was conducted...
Abstract Root-knot nematode (RKN; Meloidogyne spp.) is a major crop pathogen worldwide. Effective resistance exists for few plant species, including that conditioned by Mi in tomato (Solanum lycopersicum). We interrogated the root transcriptome of resistant (Mi+) and susceptible (Mi–) cultivars ‘Motelle’ ‘Moneymaker,’ respectively, during time-course infection Mi-susceptible RKN species incognita Mi-resistant hapla. In absence infection, only single significantly regulated gene, encoding...
In plants, most disease resistance genes encode nucleotide binding Leu-rich repeat (NLR) proteins that trigger a rapid localized cell death called hypersensitive response (HR) upon pathogen recognition. The maize (Zea mays) NLR protein Rp1-D21 derives from an intragenic recombination between two NLRs, Rp1-D and Rp1-dp2, confers autoactive HR in the absence of infection. From previous quantitative trait loci genome-wide association study, we identified single-nucleotide polymorphism locus...
The epithelial response is critical for intestinal defense against Cryptosporidium, but poorly understood. To uncover the host strategy we examined transcriptional of cells (IECs) to C parvum in experimentally infected piglets by microarray. Up-regulated genes were dominated targets interferon (IFN) and IFN-λ3 was up-regulated significantly piglet mucosa. Although IFN-λ has been described as a mediator viral pathogens, there limited knowledge any role nonviral pathogens. Accordingly, aim...
Background and Purpose— Stroke represents a significant cause of morbidity mortality after cardiac surgery. Although the risk stroke varies according to both patient procedural factors, impact genetic variants on is not well understood. Therefore, we tested hypothesis that specific polymorphisms are associated with an increased Methods— Patients undergoing surgery utilizing cardiopulmonary bypass were studied. DNA was isolated from preoperative blood analyzed for 26 different...
Old English Sheepdogs and Gordon Setters suffer from a juvenile onset, autosomal recessive form of canine hereditary ataxia primarily affecting the Purkinje neuron cerebellar cortex. The clinical histological characteristics are analogous to ataxias in humans. Linkage genome-wide association studies on cohort related identified region CFA4 strongly associated with disease phenotype. Targeted sequence capture next generation sequencing an A C single nucleotide polymorphism (SNP) located at...
Cancers of the mesothelium, such as malignant mesothelioma (MM), historically have been attributed solely to exposure asbestos. Recent large scale genetic and genomic functional studies now show that approximately 20% all human mesotheliomas are causally linked highly penetrant inherited (germline) pathogenic mutations in numerous cancer related genes. The rarity these humans makes it difficult perform statistically conclusive understand their biological effects. This has created a...
There has been much recent interest in describing the patterns of linkage disequilibrium (LD) along a chromosome. Most empirical studies that have examined this issue concentrated on LD between collections pairs markers and not considered joint effect group beyond these pairwise connections. Here, we examine many different defined by both multilocus terms. The were chosen part examining those seen real data. We how changes affect power to detect association when performing single-marker...
Summary A spergillus flavus is an opportunistic fungal pathogen that infects maize kernels pre‐harvest, creating major human health concerns and causing substantial agricultural losses. Improved control strategies are needed, yet progress hampered by the limited understanding of mechanisms infection. series studies were designed to investigate localization, morphology transcriptional profile . during internal seed colonization. Results from these indicate capable infecting all tissues...