A5071485354- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Digital Holography and Microscopy
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Optical measurement and interference techniques
- Optical Coherence Tomography Applications
- Analytical Chemistry and Sensors
- Adaptive optics and wavefront sensing
- Advanced Optical Sensing Technologies
- Near-Field Optical Microscopy
- Advanced Optical Imaging Technologies
- Brain Tumor Detection and Classification
- Spectroscopy Techniques in Biomedical and Chemical Research
- AI in cancer detection
- Atomic and Subatomic Physics Research
- Photoreceptor and optogenetics research
- Force Microscopy Techniques and Applications
- Advanced Neural Network Applications
- Advanced Biosensing Techniques and Applications
Qiqihar Medical University
2025
Southern University of Science and Technology
2020-2024
Jiangsu University
2018-2020
Point spread function (PSF) engineering is an important technique to encode the properties (e.g., 3D positions, color, and orientation) of a single molecule in shape PSF, often with help programmable phase modulator. A deformable mirror (DM) currently most widely used modulator for fluorescence detection as it shows negligible photon loss. However, relies on careful calibration precise wavefront control. Therefore, design optimal PSF not only theoretical calculation maximum information...
Liver and tumor segmentation is an important technology for the diagnosis of hepatocellular carcinoma. However, most existing methods struggle to accurately delineate boundaries liver due significant differences in their shapes, sizes, distributions, which leads unclear contour incorrect delineation lesion area. To address this gap, we propose a hybrid gabor attention convolution transformer interaction network with hierarchical monitoring mechanism segmentation, named HyborNet. Generally,...
Since the 1990s, continuous technical and scientific advances have defied diffraction limit in microscopy enabled three-dimensional (3D) super-resolution imaging. An important milestone this pursuit is coherent utilization of two opposing objectives (4Pi geometry) its combination with microscopy. Herein, we review recent progress 4Pi nanoscopy, which provides a 3D, non-invasive, diffraction-unlimited, isotropic resolution transparent samples. This includes both targeted stochastic switching...
The current limitations of single-molecule localization microscopy (SMLM) in deep tissue imaging, primarily due to depth-dependent aberrations caused by refractive index (RI) mismatch, present a significant challenge achieving high-resolution images at greater depths. To extend the imaging depth, we optimized buffer SMLM with RI matched that objective immersion medium and systematically evaluated five different RI-matched buffers, focusing on their impact blinking behavior red-absorbing dyes...
Single-molecule localization microscopy (SMLM) has improved optical resolution to the range of several tens nanometres with high contrast, making it possible noninvasively resolve nanoscale biological structures using fluorescence microscopy.1 In SMLM (Figure 1A), small subsets fluorescent molecules are randomly activated ‘blink’ due photon-switching property fluorophores. Due diffraction light, each blinking event will form an image point spread function (PSF), typically a width ∼200-300...
Abstract The recent development of single molecule imaging techniques has enabled not only high accuracy spatial resolution but also information rich functional imaging. Abundant the molecules can be encoded in its diffraction pattern and extracted precisely (e.g. 3D position, wavelength, dipole orientation). However, sophisticated dimensional point spread function (PSF) modeling analyzing methods have greatly impeded broad accessibility these techniques. Here, we present a graphics...
Resolution of single molecule localization microscopy (SMLM) depends on the accuracy, which can be improved by utilizing engineered point spread functions (PSF) with delicate shapes. However, intrinsic pixelation effect detector sensor will deteriorate PSFs under different sampling rates. The influence to achieved 3D accuracy for PSF shapes signal background ratio (SBR) and pixel dependent readout noise has not been investigated in detail so far. In this work, we proposed a framework...
Single-molecule localization microscopy (SMLM) in a typical wide-field setup has been widely used for investigating sub-cellular structures with super resolution. However, field-dependent aberrations restrict the field of view (FOV) to only few tens micrometers. Here, we present deep learning method precise spatially variant point emitters (FD-DeepLoc) over large FOV covering full chip modern sCMOS camera. Using graphic processing unit (GPU) based vectorial PSF fitter, can fast and...
Abstract The current limitations of single-molecule localization microscopy (SMLM) in deep tissue imaging, primarily due to depth-dependent aberrations caused by refractive index (RI) mismatch, present a significant challenge achieving high-resolution images at greater depths. To extend the imaging depth, we optimized buffer SMLM with RI matched objective immersion medium, and systematically evaluated five different buffers, focusing on their impact blinking behavior red-absorbing dyes...
Abstract Resolution of single molecule localization microscopy (SMLM) depends on the accuracy, which can be improved by utilizing engineered point spread functions (PSF) with delicate shapes. However, intrinsic pixelation effect detector sensor will deteriorate PSFs under different sampling rates. The influence to achieved 3D accuracy for PSF shapes signal background ratio (SBR) and pixel dependent readout noise has not been investigated in detail so far. In this work, we proposed a...
In this paper, a method for characterizing solution concentration variation based on quantitative phase imaging (QPI) is proposed with advantages of visualization, non-invasiveness and high speed. Through the system, full-field shift to reference sample obtained, sampling points are determined by taking modulus squared gradient. Phase experiments CaCl2 solutions implemented verify feasibility method. The experimental results demonstrate that optical distribution can be used characterize...
A neuron is the basic unit of structure and function biological nervous system. The detection neuronal firing developed rapidly in recent years. However, cell activity may be affected by most technologies. Considering that concentration calcium ion body plays a key role modulating solution refractive index closely related to its concentration, optical phase information employed this paper describe rhythm with label-free approach. Based on associated parameters, model soma synapses presented....
Point spread function (PSF) engineering is an important technique to encode the properties (e.g., 3D positions, color, and orientation) of single molecule in shape PSF, often with help a programmable phase modulator. Deformable mirror (DM) currently most widely used modulator for fluorescence detection as it shows negligible photon loss. However, relies on careful calibration precise wavefront control. Therefore, design optimal PSF not only theoretical calculation maximum information...