A5051784579- Anesthesia and Neurotoxicity Research
- Intensive Care Unit Cognitive Disorders
- COVID-19 Clinical Research Studies
- Anesthesia and Sedative Agents
- Monoclonal and Polyclonal Antibodies Research
- Melanoma and MAPK Pathways
- Hypothalamic control of reproductive hormones
- Immune Response and Inflammation
- Respiratory Support and Mechanisms
- Immune cells in cancer
- Biochemical and Structural Characterization
- Long-Term Effects of COVID-19
- Receptor Mechanisms and Signaling
- Computational Drug Discovery Methods
- Protein Structure and Dynamics
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Biochemical and Molecular Research
- Quinazolinone synthesis and applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Protein Kinase Regulation and GTPase Signaling
- COVID-19 and Mental Health
- Fuel Cells and Related Materials
- NF-κB Signaling Pathways
- Cardiac Ischemia and Reperfusion
- Synthesis and biological activity
Fudan University Shanghai Cancer Center
2021-2025
Renji Hospital
2018-2023
Fudan University
2020-2022
Ono Pharmaceutical (United States)
2007-2022
Research & Development Institute
2013-2022
Shanghai Medical College of Fudan University
2021
Shanghai Jiao Tong University
2019-2020
Merck Serono (Italy)
2014-2015
Reproductive Science Center
2003-2005
Columbia University
1995-2000
FSH, a glycoprotein hormone, and the FSH receptor (FSHR), G protein-coupled receptor, play central roles in human reproduction. We report crystal structure of complex with entire extracellular domain FSHR (FSHR ED ), including enigmatic hinge region that is responsible for signal specificity. Surprisingly, does not form separate structural unit as widely anticipated but part integral . In addition to known hormone-binding site, provides interaction sites hormone: sulfotyrosine (sTyr) site...
Amino acid sequence alignments of cholinesterases revealed that 6 14 aromatic amino residues lining the active center gorge acetylcholinesterase are replaced by aliphatic in butyrylcholinesterase. The Y337 (F330) mammalian acetylcholinesterase, which is A328 human butyrylcholinesterase, implicated binding ligands such as huperzine A, edrophonium, and acridines one end bisquaternary compounds BW284C51 decamethonium. may sterically hinder phenothiazines ethopropazine, contains a bulky...
Background: Glycoprotein hormones influence the development and function of ovary, testis thyroid by binding to specific high-affinity receptors. The extracellular domains these receptors are members leucine-rich repeat (LRR) protein superfamily responsible for binding. crystal structure a glycoprotein hormone, namely human choriogonadotropin (hCG), is known, but neither receptor structure, mode hormone binding, nor mechanism activation, have been established.Results Despite very low...
A Ramachandran plot is a visual representation of the main-chain conformational tendencies an amino acid. Despite forty years research, shape plots still matter debate. The issue in making based on experimental data deciding whether sparse represent genuine conformations. We present here simple solution to settle ambiguities data, and explain how we verified accuracies our using independent dataset. To obtain results, then measured pair-wise distances among acids, showed that relationships...
Follicle-stimulating hormone receptor (FSHR), a G-protein coupled receptor, is an important drug target in the development of novel therapeutics for reproductive indications. The FSHR extracellular domains were observed crystal structure as trimer, which enabled us to propose model activation mechanism. predicts that binds Asnα(52)-deglycosylated FSH at 3-fold higher capacity than fully glycosylated FSH. It also that, upon dissociation trimer into monomers, binding FSH, but not...
The myelination is a critical process during brain development. This study aimed to explore the impact of volatile anesthetic sevoflurane on developing and role microglial activation in this process. Neonatal C57BL/6J mice were exposed at their postnatal 6-8 days. Neurobehavioral tests used assess fine motor cognitive functions. Myelination hippocampus (HC) corpus callosum (CC), as well activation, determined by western blotting immunostaining. Lipid droplets assessed Oil-Red-O Bodipy...
Abstract The B cell survival factor (TNFSF13B/BAFF) is often elevated in autoimmune diseases and targeted the clinic for treatment of systemic lupus erythematosus. BAFF contains a loop region designated flap, which dispensable receptor binding. Here we show that flap has two functions. In addition to facilitating formation highly active 60-mer as shown previously, it also converts binding TNFRSF13C (BAFFR) into signaling event via oligomerization individual BAFF-BAFFR complexes. Binding...
Several lines of evidence support the hypothesis that c-Jun N-terminal kinases (JNKs) play a critical role in wide range disease states including cell death (apoptosis)-related and inflammatory disorders (epilepsy, brain, heart renal ischemia, neurodegenerative diseases, multiple sclerosis, rheumatoid arthritis, bowel syndrome). The screening compound collection led to identification 2-(benzoylaminomethyl)thiophene sulfonamide (AS004509, I) as potent selective JNK inhibitor. Chemistry...
Abstract Background Studies have provided some evidence that pain is a risk factor for postoperative delirium (POD). Therefore, we investigated the relationship between preoperative and POD after noncardiac surgery. Methods was assessed with Montreal Cognitive Assessment, cognition Mini-Mental State Examination. Plasma C-reactive protein (CRP) detected by enzyme-linked immunosorbent assay before Preoperative classified its duration surgery as chronic (lasting more than 1 month), acute less...
Abstract Background Postoperative cognitive dysfunction ( POCD ) is consistently associated with increased morbidity and mortality, which has become a major concern of patients caregivers. Although occurs mainly in aged patients, it happens at any age. Previous studies demonstrated that anesthesia/surgery had no effects on reference memory adult mice. However, whether impairs working remains unclear. Working deficit would result many deficits executive function. We hypothesized impaired the...
The binding of programmed death ligand 1 protein (PD-L1) to its receptor (PD-1) mediates immunoevasion in cancer and chronic viral infections, presenting an important target for therapeutic intervention. Several monoclonal antibodies targeting the PD-L1/PD-1 signaling axis are undergoing clinical trials; however, epitopes these have not been described. We combined orthogonal approaches localize characterize epitope a antibody directed against PD-L1 at good resolution with high confidence....
The potential protective role of eosinophils in the COVID-19 pandemic has aroused great interest, given their virus clearance function and infection resistance asthma patients to this coronavirus. However, it is unknown whether eosinophil counts could serve as a predictor severity COVID-19.A total 1004 with confirmed who were admitted Leishenshan Hospital Wuhan, China, enrolled study, including 905 general ward 99 intensive care unit (ICU). We reviewed medical data analyze association...