Karel Paukner

ORCID: 0000-0003-0150-0667
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About
Contact & Profiles
Research Areas
  • Adipokines, Inflammation, and Metabolic Diseases
  • Cancer Cells and Metastasis
  • 3D Printing in Biomedical Research
  • Cancer, Lipids, and Metabolism
  • Chronic Kidney Disease and Diabetes
  • Cardiovascular Disease and Adiposity
  • Renal and Vascular Pathologies
  • Pluripotent Stem Cells Research
  • Cholesterol and Lipid Metabolism
  • Insect and Pesticide Research
  • Helicobacter pylori-related gastroenterology studies
  • Rabbits: Nutrition, Reproduction, Health
  • Peroxisome Proliferator-Activated Receptors
  • Effects of Environmental Stressors on Livestock
  • Immune cells in cancer
  • Caveolin-1 and cellular processes
  • Kruppel-like factors research
  • Liver physiology and pathology
  • Tissue Engineering and Regenerative Medicine
  • Animal Behavior and Welfare Studies
  • Gastrointestinal motility and disorders
  • Gut microbiota and health
  • Inflammation biomarkers and pathways
  • Diabetes and associated disorders
  • Animal Nutrition and Physiology

Institute of Clinical and Experimental Medicine
2022-2025

University of Veterinary Sciences Brno
2022-2024

Saints Cyril and Methodius University of Skopje
2023

National Medical Research Center of Cardiology
2023

Charles University
2022-2023

Institute for Atherosclerosis Research
2023

Ministry of Health of the Russian Federation
2023

University of Illinois Urbana-Champaign
2023

Ghent University
2023

International Livestock Research Institute
2023

While two-dimensional (2D) cell cultures, such as Caco-2 and Madin-Darby canine kidney (MDCK) cells are widely used in a variety of biological models, these vitro systems present inherent limitations replicating the complexities vivo biology. Recent progress three-dimensional organoid technology has potential to address limitations. In this study, characteristics conventional 2D culture were compared those intestinal organoids (enteroids, ENT colonoids, COL). Light microscopy transmission...

10.20944/preprints202501.0574.v1 preprint EN 2025-01-08

While two-dimensional (2D) cell cultures, such as Caco-2 and Madin–Darby canine kidney (MDCK) cells are widely used in a variety of biological models, these vitro systems present inherent limitations replicating the complexities vivo biology. Recent progress three-dimensional organoid technology has potential to address limitations. In this study, characteristics conventional 2D culture were compared those intestinal organoids (enteroids, ENT, colonoids, COL). Light microscopy transmission...

10.3390/biology14030270 article EN cc-by Biology 2025-03-06

Abstract Background Albuminuria, an important marker of decreased kidney function in chronic disease (CKD), is not routinely used for CKD detection or proteinuria appearance. Its relationships with biochemical parameters and blood pressure dogs are poorly understood. Objectives This study aimed to evaluate the relationship albuminuria various markers, its correlation urinary protein creatinine ratio (UPC), hypertension early stages CKD. It also sought determine usability albumin (UAC)...

10.1002/vms3.1403 article EN cc-by-nc Veterinary Medicine and Science 2024-02-28

Organoids are 3-dimensional (3D) self-assembled structures capable of replicating the microanatomy and physiology epithelial components their organ origin. Adult stem cell (ASC) derived organoids from liver have previously been shown to differentiate into primarily mature cholangiocytes, partial differentiation functional hepatocytes can be promoted using specific media compositions. While full morphological ASCs has not yet reported for any species, approximated various Six formulations...

10.1016/j.heliyon.2024.e28420 article EN cc-by Heliyon 2024-03-25

The high mortality of coronary heart disease (CHD) among Czech men-one the highest worldwide-began to decline in 1991 soon after abolition government subsidies all foodstuffs rich animal fat. As participants WHO MONICA Project, we were able analyze CHD risk factors just before and this major economic change. We had previously documented that originally subsidized prices decreased fat consumption consequently non-HDL cholesterol concentrations population. By early 1990s, no progress been made...

10.3390/biomedicines10112871 article EN cc-by Biomedicines 2022-11-09

ABSTR ACTdifferences in the affinity of substrates.BCRP is suggested to increase elimination certain xenobiotics milk, posing a risk for offspring and consumers dairy products.Knowledge species differences are essential investigate allow appropriate assessment individual species.In this research we developed species-specific recombinant BCRP-vesicles potential interaction with BCRP.Materials Methods: Inside-out membrane vesicles were isolated from stable infected human embryonic kidney (HEK)...

10.1111/jvp.13329 article EN Journal of Veterinary Pharmacology and Therapeutics 2023-06-01
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