A5101925321- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Reproductive Health and Technologies
- Hepatitis B Virus Studies
- Cervical Cancer and HPV Research
- Bone health and treatments
- Myasthenia Gravis and Thymoma
- Neuroscience and Neuropharmacology Research
- Pericarditis and Cardiac Tamponade
- Genetic and Kidney Cyst Diseases
- Epigenetics and DNA Methylation
- Demographic Trends and Gender Preferences
- Ectopic Pregnancy Diagnosis and Management
- Circular RNAs in diseases
- Heat shock proteins research
- Cystic Fibrosis Research Advances
- Hormonal and reproductive studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Oral and gingival health research
- Infective Endocarditis Diagnosis and Management
- Reproductive biology and impacts on aquatic species
- Cancer-related molecular mechanisms research
- Neonatal Respiratory Health Research
- Connective tissue disorders research
First Affiliated Hospital of Wenzhou Medical University
2012-2023
Wenzhou Medical University
2009-2023
Zhejiang Academy of Medical Sciences
2012
To investigate effect of sperm DNA fragmentation (SDF) on clinical outcomes assisted reproductive technology in women with normal ovarian reserve (NOR) versus reduced (ROR).Retrospective study.University-affiliated tertiary teaching hospital.A total 2,865 consecutive couples undergoing their first vitro fertilization (IVF) or intracytoplasmic injection (ICSI) cycle.SDF assessed using chromatin dispersion samples 1-2 months before treatment.SDF, IVF, and ICSI outcomes.The grouping criteria...
During the last decades, many studies have shown possible influence of sperm DNA fragmentation on assisted reproductive technique outcomes. However, little is known about impact clinical outcome frozen-thawed embryo transfer (FET) from cycles conventional in vitro fertilization (IVF) and intra-cytoplasmic injection (ICSI). In present study, relationship between (SDF) FET outcomes IVF ICSI was analyzed. A total 1082 with cleavage stage embryos (C-FET) (855 227 ICSI) 653 blastocyst (B-FET)...
Congenital bilateral absence of the vas deferens (CBAVD) is a frequent cause obstructive azoospermia, and mutations cystic fibrosis transmembrane conductance regulator (CFTR) gene have also been frequently identified in patients with CBAVD.However, distribution CFTR polymorphisms M470V, poly-T, TG-repeats F508del mutation Chinese CBAVD population presumed low (CF) frequency remains to be evaluated.Samples obtained from 109 infertile males 104 normal controls were analyzed for presence...
This study was designed to determine whether HCO(3)(-) and Cl(-) are required for the activation of GABA(A) receptor/Cl(-) channel (GBRC) by GABA subsequent capacitation rat sperm. Spermatozoa from adult Sprague Dawley rats were incubated in four different media: modified complete fertilization medium (mRFM), Cl(-)-deficient (Cl(-)-DF) mRFM, HCO(3)(-)-DF Cl(-)-DF with or without GBRC agonists (GABA progesterone) antagonists (bicuculline picrotoxin) 0-6 h under capacitating conditions. Sperm...
Abstract: Phospholipase A2 (PLA 2 ) plays a major role during acrosomal exocytosis (AE) in mammalian spermatozoa, but the identity of PLA subtypes present spermatozoa remains elusive. This study explored whether secretory Group IID (sPLA ‐IID) isoform is human and it involved AE. Localization expression sPLA ‐IID were by immunofluorescence staining Western blot analysis. Occurrence AE was evaluated triple staining, arachidonic acid (AA) levels quantified gas chromatography—mass spectrometry....
Progesterone (P4 ) is crucial for the physiological function of spermatozoa. In study, we investigated correlation between P4 -induced sperm acrosome reaction (AR) and parameters including progressive motility, normal morphology DNA fragmentation (SDF), compared in vitro fertilization (IVF) predictive values these indicators based on multivariate regressions analysis receiver operator characteristics (ROC) curve analyses. The results demonstrated a negative AR SDF, with -9.05 (-17.25 to...
To explore the molecular mechanism for a family with hereditary X-linked spondyloepiphysealdysplasia tarda (SEDT).For 3 affected males and 2 obligate carrier females from family, exons to 6 of SEDL gene were amplified PCR sequenced.In three patients, deletional mutation (c.267_271delAAGAC) in exon 5 has been identified, which caused frameshift protein product.c.267_271delAAGAC probably underlies disease this family.
The association of single nucleotide polymorphisms (SNPs) in heat shock protein 90 (HSP90) genes with idiopathic male infertility remains unclear. In this study, the five selected SNPs HSP90AA1 namely rs10133307, rs10873531, rs11547523, rs11621560 and rs7145597 were genotyped 116 infertile males 185 ethnically matched fertile using Sequenom MassARRAY assay. role these was then studied multiple genetic models. We observed that genotype distribution (p = .028) allelic frequency .032)...
To investigate variation of sperm DNA fragmentation index (DFI) in male partners infertile couples.A total 539 males between April 2009 and 2012 were analyzed. At least one repeated routine semen analysis test performed for each sample by chromatin dispersion (SCD) following World Health Organization guidelines. Coefficient (CV) DFI was calculated.Respectively, 1, 2, 3 4 SCD analyses carried out on 473, 59, 6 1 samples. The median interval the first measurements 3.0 (1.0-11.0) months. For...
Objective To investigate the effects of inhalation anesthetics on human sperm motility and capacitation in vitro. Methods Sperm samples were obtained from normal adults prepared with discontinuous percoll gradient centrifugation technique. The incubated for 5 h an airtight glass container filledwith 5% CO2-95% air at 37 ℃ or without sevoflurane (SEV 2%, 4% ) isoflurane (ISO 1.1%, 2.2% ).Then was examined vitro 37℃ analyzed by computer-assisted analysis (CASA), including (a + b)%, curvilinear...
Objective To investigate the correlation between seminal plasma hepatitis B virus(HBV) DNA copy and semen parameters sperm fragmentation(SDF). Methods The HBV-DNA was detected by real-time PCR in 148 infertility males, those with serum above (positive) or below (negative) 5.0×102U/ml were analyzed respectively parameters, morphology fragmentation(SDF). Results Of male, 60 (40.5%) positive, of positive patients, 56 (93.3%) e antigen(HBeAg) which higher than negative males...