A5043935776- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- Cancer-related gene regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Chronic Myeloid Leukemia Treatments
- Handwritten Text Recognition Techniques
- Immune cells in cancer
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Vehicle License Plate Recognition
- Ubiquitin and proteasome pathways
- Image and Object Detection Techniques
- Immune Cell Function and Interaction
- Simulation and Modeling Applications
- Infrastructure Maintenance and Monitoring
- Inflammasome and immune disorders
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Nuclear Structure and Function
- Image Processing and 3D Reconstruction
Central People's Hospital of Zhanjiang
2019-2025
Guangdong Medical College
2019-2024
Beijing University of Posts and Telecommunications
2022-2023
Hangzhou Normal University
2023
Target (United States)
2023
Sir Run Run Shaw Hospital
2023
Zhejiang University
2004-2023
University of Pittsburgh
2014-2020
Affiliated Hospital of Guangdong Medical College Hospital
2020
First Affiliated Hospital Zhejiang University
2012-2016
Abstract N6-methyladenosine (m6A), the most abundant modification in mRNAs, has been defined as a crucial modulator progression of acute myelogenous leukemia (AML). Identification key regulators m6A modifications AML could provide further insights into biology and uncover more effective therapeutic strategies for patients with AML. Here, we report overexpression YTHDF1, an reader protein, human samples at protein level enrichment stem cells (LSC). Whereas YTHDF1 was dispensable normal...
N6-methyladenosine (m6A) modification accounts for the most prevalent mRNA internal and has emerged as a widespread regulatory mechanism in multiple physiological processes. We address role of methyltransferase-like protein 3 (METTL3) neutrophil activation. METTL3 controls release from bone marrow to circulation through surface expression CXC chemokine receptor 2 (CXCR2) Toll-like 4 (TLR4) signaling-dependent manner lipopolysaccharide (LPS)-induced endotoxemia. show that TLR4 is modified by...
Abstract BACKGROUND Circulating microRNA (miRNAs) have been shown to the potential as noninvasive diagnosis markers in several types of cancers. In this study, we investigated whether circulating miRNAs could be used prostate cancer (CaP) a Chinese patient population. METHODS Illumina's Human v2 miRNA microarray was analyze levels small set patients [25 CaP, 17 benign prostatic hyperplasia (BPH)] an effort identify CaP‐specific miRNAs. The identified were further examined by quantitative...
Abstract Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is characterised by an uncontrolled inflammatory response, and current treatment strategies have limited efficacy. Although the protective effect of M2‐like macrophages (M2φ) their extracellular vesicles (EVs) has been well‐documented in other diseases, role M2φ‐derived EVs (M2φ‐EVs) pathogenesis ALI/ARDS remains poorly understood. The present study utilised a mouse model lipopolysaccharide‐induced ALI to first...
The mechanisms underlying the potential for aggressive behavior of prostate cancer (PCa) remain elusive. In this study, whole genome and/or transcriptome sequencing was performed on 19 specimens PCa, matched adjacent benign tissues, blood specimens, and organ donor prostates. A set novel fusion transcripts discovered in PCa. Eight these were validated through multiple approaches. occurrence then analyzed 289 samples from three institutes, with clinical follow-up ranging 1 to 15 years....
Abstract EGFR phosphorylation is required for TLR4-mediated macrophage activation during sepsis. However, whether and how intracellular transported endotoxemia have largely been unknown. Here, we show that LPS promotes high levels cell surface expression of in macrophages through two different transport mechanisms. On one hand, Rab10 EEA1-mediated the membrane translocation from Golgi. other prevents its endocytosis a kinase activity-dependent manner. Erlotinib, an tyrosine inhibitor,...
Abstract Obesity, one of the most serious public health issues, is caused by imbalance energy intake and expenditure. N(6)-methyladenosine (m 6 A) RNA modification has been recently identified as a key regulator obesity, while detailed mechanism elusive. Here, we find that YTH binding protein 1 (YTHDF1), an m A reader, acts essential white adipose tissue metabolism. The expression YTHDF1 decreases in male mice fed high-fat diet. Adipocyte-specific Ythdf1 deficiency exacerbates...
Abstract Prostate cancer (PCa) is a highly common type of malignancy and affects millions men in the world since it easy to recur or emerge therapy resistance. Therefore, urgent find novel treatments for PCa patients. In current study, we found that tegaserod maleate (TM), an FDA-approved agent, inhibited proliferation, colony formation, migration as well invasion, caused arrest cell cycle, promoted apoptosis cells vitro. addition, TM suppressed tumor growth cell-derived xenograft (CDX)...
Abstract 3-Methylcytidine (m3C), a prevalent modification of transfer RNAs (tRNAs), was recently identified in eukaryotic messenger (mRNAs). However, its precise distribution and formation mechanisms mRNAs remain elusive. Here, we develop novel approach, m3C immunoprecipitation sequencing (m3C-IP-seq), utilizing antibody enrichment to profile the methylome at single-nucleotide resolution. m3C-IP-seq captures 12 cytoplasmic tRNA isoacceptors 2 mitochondrial containing modifications. Moreover,...
Upregulation of RNA polymerase (Pol) III products, including tRNAs and 5S rRNA, in tumor cells leads to enhanced protein synthesis formation, making it a potential target for cancer treatment. In this study, we evaluated the inhibition Pol transcription by triptolide anti-cancer effect drug colorectal tumorigenesis.The on development was assessed mouse models, 3D organoids, cultured cells. Colorectal were treated with triptolide. measured real-time quantitative chain reaction (PCR). The...
Creating personal Chinese handwritten font library is a very time-consuming job, with the majority of time spent on users manually writing large number characters. To dramatically cut down cost, we propose an efficient solution to generate fonts by effectively reusing sample characters that write. Our first builds Character Radical Composition Model based images standard printed The use contour curve radical clustering approach facilitates critical task learning model. We then model decide...
The induction of beige adipocytes in white adipose tissue (WAT), also known as WAT beiging, improves glucose and lipid metabolism. However, the regulation beiging at posttranscriptional level remains to be studied. Here, we report that METTL3, methyltransferase N6-methyladenosine (m6A) mRNA modification, is induced during mice. Adipose-specific depletion Mettl3 gene undermines impairs metabolic capability mice fed with a high-fat diet. Mechanistically, METTL3-catalyzed m6A installation on...
Abstract Background Beyond their crucial role in hemostasis, platelets possess the ability to regulate inflammation and combat infections through various mechanisms. Stringent control of macrophage activation is essential during innate immune responses sepsis. Macrophages are considered phagocytic cells that aid elimination pathogens. Platelet interactions with monocytes-macrophages known be significant response against bacterial infections, but primary mediator driving these remains...
Glibenclamide displays an anti-inflammatory response in various pulmonary diseases, but its exact role lipopolysaccharide- (LPS-) induced acute lung injury (ALI) or respiratory distress syndrome (ARDS) remains unknown. Herein, we aimed to explore the effect of glibenclamide vivo and vitro on development LPS-induced ALI a mouse model. LPS stimulation resulted increases score, wet/dry ratio, capillary permeability lungs, as well total protein concentration, inflammatory cells, cytokines...
This study was designed to determine whether HCO(3)(-) and Cl(-) are required for the activation of GABA(A) receptor/Cl(-) channel (GBRC) by GABA subsequent capacitation rat sperm. Spermatozoa from adult Sprague Dawley rats were incubated in four different media: modified complete fertilization medium (mRFM), Cl(-)-deficient (Cl(-)-DF) mRFM, HCO(3)(-)-DF Cl(-)-DF with or without GBRC agonists (GABA progesterone) antagonists (bicuculline picrotoxin) 0-6 h under capacitating conditions. Sperm...
Purpose: Particulate matter (PM) has been implicated as a risk factor for airway injury. However, the molecular mechanisms remain largely unclear. The goal of this study was to determine whether sirtuin1 (SIRT1), an anti-inflammatory and antiaging protein, protects against PM-induced inflammation. Methods: effect SIRT1 on inflammation assessed by using in vivo models induced PM vitro culture human bronchial epithelial (HBE) cells exposed PM, resveratrol (SIRT1 activator), or both. Results:...