Abstract Silica nanoparticles (SiNPs) have been reported to induce pulmonary fibrosis (PF) with an unknown mechanism. Recently, the activation of autophagy, a lysosome-dependent cell degradation pathway, by SiNPs has identified in alveolar epithelial cells (AECs). However, underlying mechanism and relevance SiNPs-induced autophagy development PF remain elusive. Here, we report that dysfunction subsequent apoptosis AECs are involved PF. engulfed enhance autophagosome accumulation both vivo...

Abstract Obesity, one of the most serious public health issues, is caused by imbalance energy intake and expenditure. N(6)-methyladenosine (m 6 A) RNA modification has been recently identified as a key regulator obesity, while detailed mechanism elusive. Here, we find that YTH binding protein 1 (YTHDF1), an m A reader, acts essential white adipose tissue metabolism. The expression YTHDF1 decreases in male mice fed high-fat diet. Adipocyte-specific Ythdf1 deficiency exacerbates...

Abstract 3-Methylcytidine (m3C), a prevalent modification of transfer RNAs (tRNAs), was recently identified in eukaryotic messenger (mRNAs). However, its precise distribution and formation mechanisms mRNAs remain elusive. Here, we develop novel approach, m3C immunoprecipitation sequencing (m3C-IP-seq), utilizing antibody enrichment to profile the methylome at single-nucleotide resolution. m3C-IP-seq captures 12 cytoplasmic tRNA isoacceptors 2 mitochondrial containing modifications. Moreover,...

Silica nanoparticles (SiO2 NPs) cause oxidative stress in respiratory system. Meanwhile, human cells launch adaptive responses to overcome SiO2 NP toxicity. However, besides a few examples, the regulation of NP-responsive proteins and their functions response remain largely unknown. In this study, we demonstrated that induced expression follistatin (FST), responsive gene, mouse lung tissue as well epithelial (A549). The levels Ac-H3(K9/18) H3K4me2, two active gene markers, at FST promoter...

Upregulation of RNA polymerase (Pol) III products, including tRNAs and 5S rRNA, in tumor cells leads to enhanced protein synthesis formation, making it a potential target for cancer treatment. In this study, we evaluated the inhibition Pol transcription by triptolide anti-cancer effect drug colorectal tumorigenesis.The on development was assessed mouse models, 3D organoids, cultured cells. Colorectal were treated with triptolide. measured real-time quantitative chain reaction (PCR). The...

Angiogenin (ANG) acts on both vascular endothelial cells and cancer cells, but the underlying mechanism remains elusive. In this study, we carried out a co-immunoprecipitation assay in HeLa identified 14 potential ANG-interacting proteins. Among these proteins, β-actin, α-actinin 4, non-muscle myosin heavy chain 9 are stress fiber components involved cytoskeleton organization movement, which prompted us to investigate of action ANG cell migration. Upon confirmation interactions between three...

The induction of beige adipocytes in white adipose tissue (WAT), also known as WAT beiging, improves glucose and lipid metabolism. However, the regulation beiging at posttranscriptional level remains to be studied. Here, we report that METTL3, methyltransferase N6-methyladenosine (m6A) mRNA modification, is induced during mice. Adipose-specific depletion Mettl3 gene undermines impairs metabolic capability mice fed with a high-fat diet. Mechanistically, METTL3-catalyzed m6A installation on...

Solid tumor development is frequently accompanied by energy-deficient conditions such as glucose deprivation and hypoxia. Follistatin (FST), a secretory protein originally identified from ovarian follicular fluid, has been suggested to be involved in development. However, whether it plays role cancer cell survival under energy-deprived remains elusive. In this study, we demonstrated that markedly enhanced the expression nucleolar localization of FST HeLa cells. The relied on its nuclear...

Nuclear translocation of angiogenin (ANG) is essential for the proliferation its target cells. ANG promotes rRNA synthesis, while whether it regulates mRNA transcription remains unknown. Using chromatin immunoprecipitation method, we have identified 12 ANG-binding sequences. One these sequences lies in estrogen receptor-related receptor gamma (ERRγ) gene which designated as ANG-Binding Sequence within ERRγ (ABSE). ABSE exhibited ANG-dependent repressor activity luciferase reporter system....

The clinical trials emerged centromere protein E inhibitor GSK923295 as a promising anticancer drug, but its function in hepatocellular carcinoma (HCC) remain needs to be fully elucidated, especially chemotherapy after hepatectomy for liver tumors. We aimed describe anti-HCC activities of and compare antiproliferative effects on regeneration partial (PH).All subjects were randomized treatment with either vehicle or GSK923295. Antitumor activity was assessed by xenograft growth assays....

The anticancer effect of zinc oxide nanoparticles (ZnO NPs) largely relies on cellular responses such as alteration gene expression. Although ZnO NPs have been reported to induce transcriptional changes, the potential affect translatome remains unknown. Using ribosome profiling, we demonstrated that transcription 78 genes and translation 1,448 are affected during one hour exposure in A549 human lung cancer cells. mitogen-activated protein kinase (MAPK) pathway is up-regulated upon NP...

Diurnal oscillations in gene expression are a hallmark of the liver internal clock and can be regulated by variety environmental stimuli. The circadian rhythm regeneration (LR) intimately linked. However, how they affect each other at transcriptomic level is mainly unknown. Here, we revealed that partial hepatectomy (PHx)-induced LR led to reprogramming rhythmic profiles as consequence disrupted BMAL1 occupation on chromatin, while core genes remained robust. Furthermore, demonstrated...

<p>The induction of beige adipocytes in white adipose tissue, also known as WAT beiging, improves glucose and lipid metabolism. However, the regulation beiging at posttranscriptional level remains to be studied. Here, we report that METTL3, methyltransferase N6-methyladenosine (m6A) mRNA modification, is induced during mice. Adipose-specific depletion <em>Mettl3</em> gene undermines impairs metabolic capability mice fed with a high-fat diet (HFD). Mechanistically,...

<p>The induction of beige adipocytes in white adipose tissue, also known as WAT beiging, improves glucose and lipid metabolism. However, the regulation beiging at posttranscriptional level remains to be studied. Here, we report that METTL3, methyltransferase N6-methyladenosine (m6A) mRNA modification, is induced during mice. Adipose-specific depletion <em>Mettl3</em> gene undermines impairs metabolic capability mice fed with a high-fat diet (HFD). Mechanistically,...

Abstract Activating Transcription Factor 3 (ATF3) is a member of the ATF/CREB family transcription factors and can be rapidly induced by DNA damage other cellular stresses. We previously found that ATF3 activates p53 binding to C-terminus tumor suppressor blocking its ubiquitination, consequently inhibits transformation. Since TP53 mutated in half human cancers mutant proteins (mutp53) acquire oncogenic activities including promoting drug resistance driving cancer cell invasion metastasis,...

Background: The induction of beige adipocytes in white adipose tissue (WAT), also known as WAT beiging, improves glucose and lipid metabolism. However, the function METTL3, methyltransferase N6-methyladenosine (m6A) mRNA modification, this process remains to be studied.Methods: Mettl3 conditional knockout mice were generated by mating Mettl3fl/fl with Adipoq-Cre mice. Beiging was successfully induced cold exposure (4 °C). histology expression thermogenic genes determined. RNA-sequencing,...