A5014945281- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Hormonal Regulation and Hypertension
- Ovarian function and disorders
- Estrogen and related hormone effects
- Effects and risks of endocrine disrupting chemicals
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- Circular RNAs in diseases
- Hepatitis B Virus Studies
- Aldose Reductase and Taurine
- Ferroptosis and cancer prognosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Circadian rhythm and melatonin
- Air Quality and Health Impacts
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Health and Contraception
- Retinoids in leukemia and cellular processes
- Reproductive System and Pregnancy
- Climate Change and Health Impacts
- Per- and polyfluoroalkyl substances research
- Hepatitis Viruses Studies and Epidemiology
- Free Radicals and Antioxidants
Wenzhou Medical University
2014-2025
First Affiliated Hospital of Wenzhou Medical University
2014-2025
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2023-2025
To investigate effect of sperm DNA fragmentation (SDF) on clinical outcomes assisted reproductive technology in women with normal ovarian reserve (NOR) versus reduced (ROR).Retrospective study.University-affiliated tertiary teaching hospital.A total 2,865 consecutive couples undergoing their first vitro fertilization (IVF) or intracytoplasmic injection (ICSI) cycle.SDF assessed using chromatin dispersion samples 1-2 months before treatment.SDF, IVF, and ICSI outcomes.The grouping criteria...
Perfluorodecanoic acid (PFDA), a C10 fluorine-containing compound, is used widely and found to be present anywhere. However, whether it has reproductive toxicity for fetal Leydig cells the underlying mechanisms remain unknown. PFDA was investigated its effects on (FLCs) following exposure 0, 1, 2.5, 5 mg/kg/days (gavage dams) from day 14 21 during gestation. The study showed that in utero medium-dose (1, 2.5 mg/kg/days) increased body weight. markedly reduced serum testosterone levels,...
During the last decades, many studies have shown possible influence of sperm DNA fragmentation on assisted reproductive technique outcomes. However, little is known about impact clinical outcome frozen-thawed embryo transfer (FET) from cycles conventional in vitro fertilization (IVF) and intra-cytoplasmic injection (ICSI). In present study, relationship between (SDF) FET outcomes IVF ICSI was analyzed. A total 1082 with cleavage stage embryos (C-FET) (855 227 ICSI) 653 blastocyst (B-FET)...
Abnormal imprinted genes methylation in spermatozoa has been shown to be associated with subfertility. However, the relationship between sperm DNA damage and specific remains unclear. In this study, levels were determined at seven loci (H19, INS-IGF2, KCNQ1, MEG3, MEST, PEG3 SNRPN) 66 semen samples using MSRE-qPCR method. The divided into two groups according threshold value (25%) of fragmentation index (DFI). We found that mean level IGF2 (cg17037101) group DFI ≥ 25% was lower than < (13.7...
Abstract Bisphenol S (BPS) is a novel bisphenol A (BPA) analogue, ubiquitous environmental pollutant that disrupts male reproductive system. Whether BPS affects Leydig cell maturation in puberty remains unclear. Male Sprague–Dawley rats (age of 35 days) were daily gavaged to 0, 1, 10, 100, and 200 mg/kg/day from postnatal days 35–56. at 1–10 higher doses markedly reduced serum testosterone progesterone levels but it significantly increased estradiol level. 100 elevated luteinizing hormone...
Hepatitis B virus (HBV) in semen is important for father-to-child transmission of HBV and has adverse effects on sperm quality. However, risk factors associated with remain unclear. Serum DNA hepatitis e antigen (HBeAg) levels may pose a semen. This study aims to examine whether serum DNA, HBeAg, surface (HBsAg) level were 151 male patients chronically infected from infertile couples included. HBsAg HBeAg determined using an electrochemiluminescence immune assay (ECLIA). seminal plasma...
Circadian rhythms have been found in some reproductive functions phenotypes but remain unclear for sperm DNA fragmentation index (DFI). The present study aims to investigate the diurnal variation of DFI mice model and men sperm. Adult male were sacrificed with Sperm Chromatin Structure Assay (SCSA) 24 hours at 6 evenly distributed time points. A cosinor pattern was observed a nadir zeitgeber 10 AM. In community population 630 semen samples collected between 8 AM 20 PM, temporal also fit −...
Curcuminoids are functional food additives, and the effect on gonadal hormone biosynthesis remains unclear. Gonads contain 3β-hydroxysteroid dehydrogenase isoforms, h3β-HSD2 (humans) r3β-HSD1 (rats), which catalyse pregnenolone into progesterone. The potency mechanisms of curcuminoids to inhibit 3β-HSD activity were explored. inhibitory was bisdemethoxycurcumin (IC50, 1.68 µM) >demethoxycurcumin (3.27 > curcumin (13.87 tetrahydrocurcumin (109.0 dihydrocurcumin octahydrocurcumin KGN cell...
Guilingji, a famous traditional Chinese medicine (TCM) formula, has been used to combat aging and male sexual dysfunction in China for centuries. To date, there little evidence-based clinical research on the use of Guilingji treat idiopathic oligo-asthenoteratozoospermia (OAT), therapeutic mechanism from metabolic perspective needs be investigated further. This was multicenter, double-blind, randomized controlled study 240 patients with OAT recruited four hospitals between January 2020 2022....
Abstract Backgrounds Hepatitis B virus infection could result in male infertility with sperm defects and dysfunction. Sertoli cells are essential for testis function play a crucial role spermatogenesis. cell death contributes to spermatogenesis impairment, leading poor quality. Ferroptosis has been implicated as mechanism of death. The issue studying the relationship between hepatitis ferroptosis not yet addressed. Objectives To explore mechanisms underlying virus‐exposed cells. Materials...
This study aims to investigate whether oral contraceptive pills (OCP) pretreatment impairs pregnancy outcomes in polycystic ovary syndrome (PCOS) women undergoing GnRH agonist protocol. A total of 1025 couples underwent their first cycle vitro fertilization. Patients were divided into protocol group (LP group) and OCP dual suppression (OC-LP group). Logistic regressions performed estimate the risk factors affecting live birth following fresh embryo transfer between groups. Frozen–thawed...
BACKGROUND:The condition of the zona pellucida can be used to predict human oocyte quality. This study investigated embryological characteristics and clinical outcomes oocytes with heterogeneous (HZP) during in vitro fertilization (IVF) intracytoplasmic sperm injection (ICSI). MATERIAL AND METHODS:This was a retrospective IVF ICSI cycles undertaken at The First Affiliated Hospital Wenzhou Medical University between June 2006 March 2016. Cycles involving HZP (HZP group) were compared those...
This study aims to compare the embryological and clinical parameters of intracytoplasmic sperm injection (ICSI) cycles using testicular versus ejaculated in male patients with elevated DNA fragmentation (SDF). A total 73 ICSI were examined couples where partner exhibited high levels SDF. was performed either or sperm. The primary outcomes rates blastocyst formation, high-quality embryo development, pregnancy. index (DFI) for (16.81 ± 17.51) significantly lower than that (56.96 17.56). While...
Background. Declining semen quality has become one of the most serious public health problems worldwide; however, spatial–temporal characteristics infertile men remain unknown. Objective. To explore in Wenzhou from 2010 to 2018 and thereby provide a scientific basis for development strategies enhance male reproductive health. Methods. We obtained 22,978 sample data Reproductive Medicine Center Urology First Affiliated Hospital Medical University Wenzhou, China. Linear regression analyses...